Antimicrobial evaluation, degree of solubility, and water sorption of universal dental adhesive incorporated with epigallocatechin-3-gallate: An In-vitro study

Paulo Goberlânio Barros Silva; Caroline Nágila Do Nascimento Terto; Rebeca Da Cunha Andrade; Argus Ramom Rolim Fernandes; Juliana Paiva Marques Lima Rolim; Jiovanne Rabelo Neri

doi:10.4103/ejgd.ejgd_137_19

European Journal of General Dentistry, Table of Contents

CC BY-NC-ND 4.0 · European Journal of General Dentistry 2020; 9(02): 79-83
DOI: 10.4103/ejgd.ejgd_137_19

Original Article

Antimicrobial evaluation, degree of solubility, and water sorption of universal dental adhesive incorporated with epigallocatechin-3-gallate: An In-vitro study

Authors

Paulo Goberlânio Barros Silva

Department of Dentistry, Christus University Center (Unichristus), Fortaleza, CE, Brazil
Caroline Nágila Do Nascimento Terto

Department of Dentistry, Christus University Center (Unichristus), Fortaleza, CE, Brazil
Rebeca Da Cunha Andrade

Department of Dentistry, Christus University Center (Unichristus), Fortaleza, CE, Brazil
Argus Ramom Rolim Fernandes

Department of Dentistry, Christus University Center (Unichristus), Fortaleza, CE, Brazil
Juliana Paiva Marques Lima Rolim

Department of Dentistry, Christus University Center (Unichristus), Fortaleza, CE, Brazil
Jiovanne Rabelo Neri

Department of Dentistry, Christus University Center (Unichristus), Fortaleza, CE, Brazil

Abstract

Background: Several strategies have been developed to reduce collagen degradation in the adhesive interface. Epigallocatechin3gallate (EGCG) has the ability to stabilize collagen, and it is effective in microbial reduction. Aims and Objectives: The objective of the present study was to evaluate the antimicrobial potential, water sorption (WS), and solubility of a universal adhesive incorporated with EGCG at concentrations of 0.02%, 0.1%, and 0.5%, respectively. Materials and Methods: Atotal of 40 specimens of composite resin disks, to which the adhesives were applied, were divided into four groups: control (without EGCG), 0.02%, 0.1%, and 0.5%. The specimens were submitted to an in vitro cariogenic challenge, inoculated with Streptococcus mutans for 3 days for biofilm formation. The generated biofilm was collected, and the colonyforming units were established. For the solubility test, the specimens using the adhesive were divided into the same groups mentioned previously (n = 10). Adhesive models were made following an ISO standard for sorption and solubility tests. Microbiological data were submitted to the analysis of variance (ANOVA), followed by the Tukey test (P < 0.05). ANOVA was used to evaluate WS and solubility, and comparisons were made by post hoc analysis by the Student–Newman–Keuls method (P < 0.05). Results: A statistical difference was observed regarding the antimicrobial potential between the groups without and with EGCG at 0.5% (P = 0.03). EGCG 0.5% presented the highest values of solubility and WS (P < 0.01 and P = 0.009, respectively). Conclusion: The addition of 0.5% EGCG was capable of inhibiting biofilm formation; however, it caused significant alteration of the solubility and sorption of the adhesive.

Keywords

Antimicrobials - dental adhesives - flavonoids - Streptococcus mutans - water solubility

Full Text

References

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