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CC BY 4.0 · Revista Brasileira de Cirurgia Plástica (RBCP) – Brazilian Journal of Plastic Surgery 2024; 39(01): 217712352023rbcp0799pt
DOI: 10.5935/2177-1235.2023RBCP0799-PT
Artigo Original

Repair of the abdominal wall with acellular bovine pericardial membranes - Part II - Histological and morphometric analyses

Article in several languages: português | English
LUIZ FERNANDO FRASCINO 
1   Hospital do Coração de São José do Rio Preto, São José do Rio Preto, SP, Brasil.
2   Instituto Frascino, Unidade de Publicação e Pesquisa, São José do Rio Preto, SP, Brasil.
,
LUCA REIS FRASCINO 
2   Instituto Frascino, Unidade de Publicação e Pesquisa, São José do Rio Preto, SP, Brasil.
3   Faculdade de Ciências Médicas da Santa Casa de São Paulo, São Paulo, SP, Brasil.
,
JORGE ALBERTO THOME 
4   Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, SP, Brasil.
,
MOACIR FERNANDES DE GODOY 
4   Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, SP, Brasil.
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▪ ABSTRACT

Introduction:

Histological analysis is the main tool for evaluating acellular bioprostheses, mostly on an experimental basis. The objective is to histologically analyze the acellular matrix of bovine pericardium in abdominal wall repairs implanted in humans.

Method:

From a series of 30 repairs with the membrane, 3 patients underwent surgical revision unrelated to the implants at 13, 22, and 23 months postoperatively, obtaining biopsies of the previously implanted areas. In addition to evaluating the basic aspects of biocompatibility and tissue neoformation, the slides were digitalized and subjected to computerized analysis with the ImageJ software to quantify the kinetics of membrane degradation associated with the analysis of the fractal dimension of the samples. The values obtained for percentages of residual membrane had their means compared by analysis of variance (ANOVA) and the unpaired Student’s T test, also used for the fractal dimension quantification values.

Results:

The biocompatibility of the material was demonstrated, with tissue neoformation, collagen deposition, and cellularized tissue with a normal appearance without important local reactions. Residual fragments of the membrane were quantified at 40%±7% at 13 months, at 20%±6% at 22 months, and at 17%±6% at 23 months postoperatively, with the analysis of the fractal dimension indicating a progressive degradation of implants, with statistical significance between 13 months and late samples.

Conclusion:

The results confirmed the functionality of the acellular bovine pericardium under different levels of mechanical stress in abdominal wall repairs in humans.

Keywords:

Extracellular matrix - Abdominal hernia - Abdominal wall - Prosthetics and implants - Surgical meshes - Bioprosthesis - Pericardium

Institution: Clínica Spani Vendramin, Belém, PA, Brazil.




Publication History

Received: 14 March 2023

Accepted: 05 December 2023

Article published online:
20 May 2025

© 2024. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution 4.0 International License, permitting copying and reproduction so long as the original work is given appropriate credit (https://creativecommons.org/licenses/by/4.0/)

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Bibliographical Record
LUIZ FERNANDO FRASCINO, LUCA REIS FRASCINO, JORGE ALBERTO THOME, MOACIR FERNANDES DE GODOY. Reparação da parede abdominal com membranas acelulares de pericárdio bovino - Parte II - Análises histológicas e morfométricas. Revista Brasileira de Cirurgia Plástica (RBCP) – Brazilian Journal of Plastic Surgery 2024; 39: 217712352023rbcp0799pt.
DOI: 10.5935/2177-1235.2023RBCP0799-PT
 

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