Summary
Introduction: Fibrin tissue adhesive, which has applications in several areas of medicine, can be
prepared by different methods.
Aim: To compare fibrin tissue adhesives prepared by 3 different methods.
Method: In this prospective experimental laboratory study, fibrin tissue adhesives prepared
by the use of plasma fibrinogen (group 1), cryoprecipitation (group 2), and precipitation
by ammonium sulfate (group 3) were tested on 15 rabbits and 10 fragments of dura mater.
The quality of the clots was assessed in terms of the success of the healing process,
local toxicity, graft adhesion capacity, and degree of adhesion of 2 fragments of
dura mater produced.
Results: All methods produced a clot with high adhesion and no toxicity, but tensile strength
testing revealed that the glue produced from the ammonium sulfate-precipitated clot
(group 3) was the strongest, requiring 39 g/cm2 to separate the fragments as opposed to 23 g/cm2 for group 2 and 13 g/cm2 for group 1.
Conclusion: All methods produced good results as far as clot formation and non-toxicity, but ammonium
sulfate precipitation produced the best tensile strength and was thus the most effective
method of preparing fibrin tissue adhesive.
Keywords
Fibrin - Fibrin Tissue Adhesive - Biological Therapy - Fibrinogen - Plasma
