Evaluation of Herb-Drug Interactions in Humans
Ginkgo
Ginkgo (Ginkgo biloba; family Ginkgoaceae), also known as maidenhair tree, is a 190 million years old,
unique species of tree with no close living relatives [15]. Pharmaceutical G. biloba (GB) products represent leave extracts and contain flavonoid glycosides (mainly quercetin,
kaempferol, isorhamnetin) and terpene lactones (ginkgolides A, B, C, J, and bilobalides).
GB products rank among the most widely used herbal products in the United States.
Oral standardized dry extracts of GB usually contain between 22–27 % flavones glycosides,
5–7 % terpene lactones, and should not exceed more than 5 ppm of ginkgolic acids,
constituents with known allergic potency [16]. Many studies have been conducted using EGb 761, a well-defined extract of GB.
GB is believed to have nootropic properties and is mainly used as a memory and concentration
enhancer and for the treatment of cognitive impairment and dementia of various origins
(e.g., Alzheimerʼs disease). GB products are also advocated for use in peripheral
arterial disease (i.e., intermittent claudication) and to improve microcirculation
in disease states such as tinnitus. GB supplements are usually taken in the range
of 40 to 240 mg per day, with recommended doses of 120 to 240 mg daily for treatment
of dementia and memory impairment.
GB preparations may confer appreciable antiplatelet effects, which are apparently
mediated by various ginkgolides. Accordingly, the most frequently reported interactions
associated with the use of GB are referring to enhancement of the PD action of platelet
inhibitors or anticoagulants. Although clinically important, these PD interactions
are beyond the scope of the present review on metabolism- and transporter-based PK
interactions.
Various and overall inconsistent in vitro effects of GB extracts or specific constituents were demonstrated regarding their
potential to alter activities of CYP enzymes. Findings suggesting either induction
or inhibition of various CYP enzymes, sometimes concentration-dependently (i.e., inhibition
at low concentrations, induction at high concentrations), sometimes in a substrate-dependent
fashion have been described. Results have been obtained in part at very high concentrations,
unlikely to be achieved at recommended doses in vivo. Collectively, the accumulated evidence from in vitro studies provides unfortunately little guidance in the reliable prediction of relevant
metabolic or transporter-based GB-mediated drug interactions in vivo.
In the meantime, however, a considerable number of clinical studies have been conducted
for the assessment of GB effects on various CYP isoforms, other drug metabolizing
enzymes, and transporters, predominantly by using specific probe drugs, thereby allowing
the identification and quantification of alterations of specific CYP enzyme and transporter
activities. Overall, we identified a total of 21 human PK studies, reporting original
drug interaction data on GB products.
Gurley and coworkers evaluated the effect of GB extract (containing 24 % flavone glycosides
and 6 % terpene lactones), given at doses of 60 mg four times daily over 28 days to
12 healthy adult subjects (6 m/6 f) [17]. In this study, CYP 3A4, 1A2, 2E1, and 2D6 activities were estimated by the use
of 1-OH-MDZ/MDZ serum ratios (1-hour sample), paraxanthine/caffeine serum ratios (6-hour
sample), 6-OH-CZX/CZX serum ratios (2-hour sample), and debrisoquine urinary recovery
ratios (8-hour collection), respectively. Comparisons of pretreatment and post-treatment
ratios indicated that GB did not result in any appreciable alteration of CYP 3A4,
1A2, and 2D6 function but appeared to modestly enhance the activity of CYP2E1 by 23 %,
although this effect was not statistically significant [17].
In a similar study in 12 elderly subjects (6 m/6 f), using the same GB product, dose
and treatment duration, as well as the same CYP probe drugs, no significant effects
of GB in terms of modulation of CYP 3A4, 1A2, 2E1, and 2D6 activities were found,
thereby not confirming the previously observed trend towards a modest CYP2E1 induction
[18].
Consistent results were reported by Markowitz and colleagues, who assessed the influence
of standardized GB extract on the activity of CYP2D6 and 3A4 in normal adult subjects,
phenotyped as CYP2D6 extensive metabolizers. As probe substrates, dextromethorphan
(CYP2D6 activity) and alprazolam (CYP3A4 activity) were coadministered orally at baseline,
and following treatment with GB (120 mg b. i. d. = 240 mg/day) for 14 days. No statistically
significant differences were found between baseline and post-GB treatment on dextromethorphan
metabolic ratios, indicating a lack of GB effect on CYP2D6 activity. For alprazolam
there was a statistically significant, albeit modest 17 % decrease in total exposure
(AUC). Based on these findings, the authors concluded that standardized extracts of
GB at recommended doses are unlikely to significantly alter the disposition of coadministered
medications primarily relying in their clearance on CYP2D6 or CYP3A4 pathways [19].
The effects of GB supplementation (90 mg/day) administered for 30 days on the steady-state
plasma concentrations of the CYP3A4 and CYP2D6 substrate donepezil were investigated
in 14 Japanese patients (6 m/8 f; age range 65–80 years) with Alzheimerʼs disease
[20]. The results of this study showed that relatively low doses of GB in elderly patients
with Alzheimerʼs disease did not alter the steady-state plasma concentrations of donepezil,
which implies that daily doses of 90 mg of ginkgo do not have appreciable inhibitory
or inducing effects on the CYP3A4- and CYP2D6-mediated metabolism of donepezil in
the target population.
Robertson and colleagues evaluated the effect of GB (120 mg b. i. d. = 240 mg/day)
for two weeks on the steady-state exposure of lopinavir and ritonavir in 14 healthy
subjects administered a lopinavir/ritonavir combination [21]. In addition, single oral doses of probe drugs MDZ and fexofenadine were administered
prior to and after 4 weeks of GB (following washout of lopinavir/ritonavir) to assess
the influence of ginkgo on CYP3A4 and P-gp activity, respectively. Lopinavir, ritonavir,
and fexofenadine exposures were not significantly affected by GB administration, while
total (AUC) and maximum (Cmax) exposure of the CYP3A4 prototypic substrate MDZ were significantly lowered by 34 %
and 31 %, respectively, relative to baseline. These results suggest that GB modestly
induces – most probably presystemic (i.e., in the gut wall) – CYP3A metabolism, as
indicated by the observed decrease in MDZ bioavailability. However, there was no change
in the exposure of lopinavir, suggesting that the modest GB-mediated induction of
CYP3A4 has most likely been superseded by ritonavirʼs potent inhibition of CYP3A4.
Thus, GB appears unlikely to reduce the exposure of ritonavir-boosted protease inhibitors.
As the H1-receptor antagonist fexofenadine is a substrate of P-gp (ABCB1), the intestinal uptake
transporters organic anion transporting polypeptides 1A2 (OATP1A2) and 2B1 (OATP2B1)
as well as the hepatic uptake transporter OATP1B1 [22], the observed unaltered disposition of fexofenadine in the study suggests that GB
is unlikely to alter the function of these transporter proteins to a significant extent
in vivo.
In a small single-dose study in 8 healthy male subjects, 240 mg of GB (Ginkgolon-24™,
powder capsules) did not alter the mean exposure to the CYP3A4 substrate nifedipine
or its major metabolite dehydronifedipine [23]. Although the observation of remarkably increased nifedipine Cmax levels in two subjects upon GB coadministration was emphasized by the authors, and
possible underlying mechanisms discussed, the known PK variability of nifedipine together
with the overall study results indicating absence of any GB effects in terms of total
nifedipine exposure (i.e., AUC) rather support the assumption of a lack of a systematic
effect of G. biloba on the bioavailability of nifedipine. This interpretation would be consistent with
reports from other GB interaction studies with CYP3A4 substrates (see above).
Uchida et al. assessed the influence of high repeat doses of GB (360 mg/d EGb 761,
Ginkgold®, given as two 60 mg tablets (120 mg) t. i. d. for 28 days = 360 mg/day)
on the PK of orally administered CYP2C9 and CYP3A4 probe drugs tolbutamide (125 mg,
single dose) and midazolam (8 mg, single dose) in 10 healthy male subjects, thereof
9 subjects with extensive metabolizer genotypes of CYP2C9 (CYP2C9*1/*1) and 1 subject
with CYP2C9*1/*3 genotype [24]. This study showed that high-dose GB treatment slightly decreased the total exposure
(AUC0-∞) of tolbutamide by 16 % and the metabolic AUC0-∞ ratio of tolbutamide to 4-hydroxytolbutamide in plasma by 17 % (p < 0.05 for both
outcomes). In contrast, total exposure (AUC0-∞) of MDZ was significantly increased by 25 % and oral clearance significantly decreased
by 26 % upon repeated high-dose GB treatment, whereby the metabolic AUC0-∞ ratio of MDZ to 1-OH-MDZ was not significantly altered. The study suggests evidence
that a minor CYP2C9 induction can be achieved with supra-therapeutic doses (360 mg/day)
of GB in vivo, and that some inhibition of intestinal CYP3A4 may go along with a modest increase
in the bioavailability of sensitive CYP3A4 substrates. Overall the study results indicate
a low potential of GB to confer CYP3A4- and CYP2C9-based metabolic drug interactions,
and thus are consistent with reports from other studies that reported no effects of
GB on the function of these enzymes at recommended doses.
By using diazepam as a model substrate of CYP2C19 and 3A4, when 10 mg single diazepam
doses were given either alone or concomitantly with oral GB (120 mg b. i. d. = 240 mg/day)
for 28 days to 12 healthy subjects, Zuo and coworkers demonstrated that total exposure
(i.e., AUC) for diazepam and the main metabolite N-desmethyldiazepam were essentially
unaltered, thereby indicating that the disposition of CYP2C19 and 3A4 substrates is
unlikely to be substantially modified by recommended doses of GB products [25].
Jiang and colleagues investigated the effects of GB on the PK and PD of warfarin in
12 healthy male subjects (eight Caucasians, four Asians) [26]. A single 25-mg dose of warfarin (Coumadin™, 5 × 5-mg tablets) was administered
to each subject with and without pretreatment with multiple doses of GB for 1 week
(Tavonin™; 40 mg tablets, each tablet containing the standardized dry extract, EGb761,
equivalent to 2 g of G. biloba leaf, 9.6 mg of ginkgo flavonglycosides, 2.4 mg of ginkgolides and bilobalide, 2 × 40 mg
tablets, t. i. d. = 240 mg/day). Dosing of GB was continued for a further week after
warfarin administration. The bioanalytics comprised enantiomer-selective quantification
of S-warfarin, which is predominantly metabolized to S-7-hydroxywarfarin by CYP2C9,
and R-warfarin, which is metabolized by CYP1A2 and CYP3A4, thereby allowing for a
separate mechanistic assessment of any potential alteration of these metabolic pathways
by concomitant GB treatment. The study demonstrated that GB, at the highest recommended
dose, had no effect on the activity of CYP1A2, CYP3A4, or CYP2C9 in healthy subjects,
as treatment did not affect the PK and clearance of both warfarin enantiomers in human
subjects. GB also did not affect the apparent volumes of distribution or protein binding
of warfarin enantiomers. PD endpoints of warfarin 25 mg single doses were also assessed
in this study, and it was shown that GB did not significantly alter blood coagulation
(i.e., INR) outcomes and platelet aggregation.
Greenblatt and colleagues used single 100 mg doses of the nonsteroidal anti-inflammatory
drug (NSAID) flurbiprofen as a probe substrate for CYP2C9, to examine the effects
of short-term GB treatment (3 doses of 2 × 60 mg tablets = 120 mg, given in 12-hour
intervals, as EGb761) in 12 healthy adult subjects (8 m/4 f), who were taking no other
medication [27]. The study showed that pretreatment of healthy subjects with usual clinical doses
of GB has no detectable effect on the PK of a single dose of flurbiprofen or on the
apparent extent of formation of the principal hydroxylated metabolite. The findings
suggest that short-term exposure to GB does not inhibit CYP2C9 activity in vivo. As the study involved only short-term exposure to GB, it was not designed to capture
possible CYP2C9 induction that could occur with long-term treatment.
Two PK studies in healthy subjects were performed using tolbutamide and diclofenac
as probe substrates of CYP2C9. No interactions between GB extract and these CYP2C9
probe substrates were observed in vivo as evidenced by the lack of effect on the steady-state pharmacokinetics of diclofenac
and the urinary metabolic ratio of tolbutamide [28].
Yin et al. examined the effects of GB on CYP2C19 and CYP3A4 metabolic pathways by
using the proton pump inhibitor omeprazole (OPZ), as a well-established CYP2C19 substrate
which undergoes CYP2C19-mediated hydroxylation to form its major metabolite 5-hydroxyomeprazole
(5-OH-OPZ), and which also undergoes sulfoxidation via CYP3A4 to form omeprazole sulfone
(OPZ-SUL) [29]. To decipher differential effects on both pathways, urinary 6β-hydroxycortisol/cortisol ratios were determined as a marker of CYP3A4 activity before
and after GB as well. The study was conducted in 18 healthy Chinese subjects genotyped
for CYP2C19 (6 homozygous EMs, 5 heterozygous EMs, and 7 homozygous PMs), and all
subjects received a single oral OPZ 40 mg dose at baseline and after a 12-day treatment
period with GB (140 mg, two 70 mg tablets b. i. d. = 280 mg/day). Results displayed
mean decreases in OPZ AUC0-∞ of 41.5 %, 27.2 %, and 40.4 % (p < 0.05 or 0.01, each compared to control) in the
homozygous EMs, heterozygous EMs and PMs, respectively. Similarly, decreases in OPZ-SUL
AUC0-∞ of 41.2 %, 36.0 %, and 36.0 % (p < 0.05 or 0.01, each compared to control), respectively,
were noted. However, no significant differences in the AUCOPZ/AUCOPZ-SUL metabolic ratio and the mean 6β-hydroxycortisol/cortisol ratio were observed before and after GB in each genotype
group. In contrast, GB increased the plasma concentrations of 5-OH-OPZ, with mean
increases of 37.5 %, 100.7 %, and 232.4 % in the homozygous EMs, heterozygous EMs
and PMs, respectively. Accordingly, a significant decrease in the AUC ratio of OPZ
to 5-OH-OPZ was observed in the homozygous EMs, heterozygous EMs, and PMs, respectively,
whereby the decrease of the metabolic ratio was greater in PMs than EMs. The study
results suggest that high-dose GB may induce OPZ hydroxylation in a CYP2C19 genotype-dependent
manner but also appears to concurrently reduce the renal clearance of 5-OH-OPZ. Taken
together, the observation that in PMs the seemingly inducing effect of GB was particularly
pronounced is difficult to interpret, because of the obvious difficulty to induce
CYP2C19 at all in PMs. On the other hand, the CYP3A4 activity was unaffected as shown
by the lack of changes in the AUCOPZ/AUCOPZ-SUL as well as 6β-hydroxycortisol/cortisol ratios before and after GB. Overall, the study indicates
that high-dose treatment with GB may significantly induce the clearance of CYP2C19
substrates, thereby possibly reducing their clinical effects.
Lei et al. examined the effects of GB (120 mg b. i. d. for 12 days) as an inducer
of CYP2C19 in 14 healthy Chinese subjects genotyped as either CYP2C19 extensive (EMs;
7 carriers of 2C19*1/2C19*1) or poor metabolizers (PMs; 7 carriers of 2C19*2/2C19*2),
by assessing the single dose PK of the orally administered CYP2C19 substrate voriconazole
(200 mg) [30]. In EMs, the median voriconazole AUC0-∞ values were 5.17 µg × h/mL and 4.28 µg × h/mL after administration of voriconazole
alone and after voriconazole with concomitant GB treatment, respectively, a 17 % difference
suggesting a trend towards a modest induction of CYP2C19 by GB, although results did
not achieve statistical significance. All other PK parameters of voriconazole such
as AUC0–24, time to reach maximum concentration (tmax), terminal disposition half-life (t1/2), and apparent oral clearance (CL/F) also did not change significantly in the presence
of GB in EMs of CYP2C19, and the results followed a similar pattern in PMs of CYP2C19.
Based on the results of this study, it can be concluded that – even at the highest
recommended doses – GB-mediated CYP2C19-based PK drug interactions appear to be weak
and may have limited clinical significance.
Recently, the effects of GB (240 mg per day, administered as two 60-mg capsules b. i. d.
for 14 days) on the activity of CYP2B6 was investigated in 14 healthy adult male Chinese
subjects (age range 19–25 years), by assessment of the single dose PK of the antidepressant
and smoking cessation drug bupropion (150 mg), which is almost exclusively metabolized
by CYP2B6 to its active metabolite hydroxybupropion. The study showed that a 14-day
oral administration of GB extract at the high end of the recommended dose range had
no statistically significant effect on the total exposure of bupropion or its active
metabolite hydroxybupropion, as measured by plasma AUC [31]. These results indicate that GB is unlikely to alter the disposition of sensitive
CYP2B6 substrates.
Mauro and coworkers assessed the effect of recommended GB doses (240 mg/day, administered
as two 40-mg tablets t. i. d. for 8 days) on the pharmacokinetics of the P-gp (ABCB1)
substrate digoxin (0.5 mg) in eight healthy human subjects (7 m/1 f, mean age 23 ± 3 yrs)
[32]. The total digoxin exposure (AUC0-∞) was observed with 21.0 ± 8.6 ng/mL × h (digoxin alone) and 25.6 ± 13.2 ng/mL × h
(digoxin + GB), indicating a trend towards a modest (i.e., 22 %) increase in digoxin
exposure associated with a 1-week GB pretreatment. Although the authors emphasized
that the digoxin AUC outcomes were not significantly different from each other, absence
of GB treatment on P-gp (ABCB1) function should not be concluded from the study due
to the lack of statistical power (small sample size).
The capacity of high-dose GB treatment (360 mg/day for 14 days) to alter the function
of P-gp (ABCB1) was examined by Fan and coworkers in 10 healthy male subjects by using
the P-gp (ABCB1) substrate talinolol (100 mg, single dose) [33]. GB increased the maximum observed plasma concentration of talinolol (Cmax) by 36 % (90 % CI 10 to 68; p = 0.025), AUC0–24 by 26 % (90 % CI 11 to 43; p = 0.008), and AUC0-∞ by 22 % (90 % CI 8 to 37; p = 0.014), respectively, without significant changes in
terminal disposition half-life (t1/2) and the time to Cmax (tmax). The authors concluded that the results indicate that repeated supra-therapeutic
doses of GB may modestly inhibit intestinal P-gp, thereby increasing the bioavailability
of sensitive P-gp transporter substrates. Comparable results were obtained by the
same group of investigators in another study of similar design [34].
The long-term administration of GB (EGb 761 120 mg/day for 90 days) to 10 healthy
adult subjects and 10 patients with type 2 diabetes mellitus (T2DM) did not significantly
affect the PK of metformin single doses (500 mg in healthy subjects and 250 to 850 mg
in T2DM patients) [35]. As metformin is a substrate of the plasma membrane monoamine transporters (PMAT),
which are localized on the luminal side of enterocytes and were recently suggested
to be the major transporter system responsible for the uptake of metformin from the
gastrointestinal tract [36], the study suggests that long-term treatment with intermediate GB doses does not
alter PMAT-mediated metformin absorption. As metformin, which is a hydrophilic organic
cation (pKa 12.4) that is not significantly metabolized in humans is a good probe
drug for organic cation transport (e.g., OCT1 and OCT3) in intestinal drug absorption,
hepatic uptake (OCT1), and is also a substrate for several other polyspecific tubular
OCT transporters in the kidney (e.g., OCTN1, OCTN2, MATE1, and MATE2-K may be involved
in the renal excretion of metformin in humans) [37], it can be concluded that GB, at the dose level investigated, is unlikely to alter
the disposition of OCT substrates to a significant extent.
Eight healthy subjects (4 m/4 f) were treated with single oral 250 mg doses of ticlopidine,
a putative substrate of the organic anion transporting polypeptide OATP-B, either
given alone or together with repeat dose GB (40 mg t. i. d. = 120/day; unspecified
product) for 3 days [38]. In this study, short-term and low-dose coadministration of GB did not significantly
change any of ticlopidineʼs PK parameters. These results are consistent with another
study in which single coadministration of GB extract (80 mg) with ticlopidine (250 mg)
in healthy male Korean subjects was not associated with any significant changes in
the PK profile of ticlopidine compared with ticlopidine administered alone [39]. The information that can be derived from both studies in terms of clinical significance
is limited, because of the low GB doses employed, and the short-term/single dose posology,
respectively.
In the most recent study, a cocktail phenotyping design was employed to assess the
interaction profile of GB extract EGb 761® on the activities of the major CYP enzymes
CYP1A2, CYP2C9, CYP2C19, CYP2D6, and CYP3A [40]. EGb 761® extract or matched placebo were administered for 8 days to 18 healthy
subjects (8 m/10 f) according to a 3-fold crossover design; total daily doses of 240 mg
EGb 761® were given in one study period following a 120 mg b. i. d. posology (= 240 mg/day),
and in the other study period 240 mg doses given q. d. in the morning. The phenotyping
cocktail was orally administered before and after the EGb 761®/placebo treatment periods
and employed the following probe substances and metrics: 150 mg caffeine (CYP1A2,
paraxanthine/caffeine plasma ratio 6-h postdose), 125 mg tolbutamide (CYP2C9, plasma
concentration 24-h postdose), 20 mg OPZ (CYP2C19, OPZ/5-OH-OPZ plasma ratio 3-h postdose),
30 mg dextromethorphan (CYP2D6, dextromethorphan/dextrorphan plasma ratio 3-h postdose),
and 2 mg of MDZ (CYP3A, MDZ plasma concentration 6-h postdose).
Point estimates of EGb 761®/placebo ratios for phenotyping metrics were close to unity
for all CYPs, except for CYP2C9 (0.834 for 120 mg b. i. d.; 0.848 for 240 mg q. d.)
and 2C19 (0.874 for 120 mg b. i. d. and 0.896 240 mg q. d.), which may suggest a weak
trend towards induction of these CYP2C family enzymes. Respective CIs for most CYP
enzymes were within the prespecified acceptance margins (i.e., 0.70–1.43) for all
ratios except CYP2C19 for EGb 761 ® 120 mg b. i. d. (90 % CI 0.681–1.122) and CYP2D6
for EGb 761 ® 240 mg q. d. (90 % CI 0.667–1.281). Taken together the data clearly
indicate the absence of any CYP inhibitory potential of 240 mg daily doses of EGb
761® extract towards CYP1A2, CYP2C9, CYP2C19, CYP2D6, and CYP3A, regardless whether
the product is given once or twice daily. The study also showed that EGb 761® extract
does not induce the activity of CYP1A2, CYP2D6, and CYP3A enzymes, and may confer
a weak induction of CYP2C9 and CYP2C19 enzymes, which, however, appears too small
to be of clinical relevance.
Taken together, besides St. Johnʼs Wort, GB appears to be at the time being, the second
best studied natural health product, in terms of the clinical investigation of its
metabolic and transporter-based PK drug interaction potential. The available set of
published mechanistic drug-drug interaction studies allows reasonable inferences on
the respective drug metabolizing enzyme and transporter effects of GB products in vivo. The information which can be derived from these studies is largely consistent, with
some minor discrepancies that may be explained by differences in study design and
populations, employed doses and treatment durations, and perhaps also differences
in the composition of products investigated.
Collectively, the available evidence indicates that recommended doses of GB up to
daily doses of 240 mg do not have significant or clinically meaningful effects on
the activity of CYP enzymes such as CYP1A2, CYP2C9, CYP2D6, CYP2B6, CYP2E1, and CYP3A4.
While data on PK alterations of CYP3A4 are not entirely consistent, sometimes suggesting
the possibility of presystemic induction of CYP3A4 with a high-dose treatment of GB,
sometimes – in turn – suggesting CYP3A4 inhibition, the overall CYP3A4-related effect
sizes described are generally weak and unlikely to be of clinical relevance unless
CYP3A4 substrates with a narrow therapeutic index are concerned.
CYP2C19 appears to be modestly inducible by GB products, with across study evidence
that this effect appears to emerge at the highest recommended dose level of 240 mg/day
[30] and may become somewhat more pronounced with increasing doses [29]. Differences in the effect sizes of CYP2C19 induction, however, may also depend
on the individual CYP2C19 genotype of subjects [29] and perhaps on substrate characteristics. The available evidence in this respect,
however, appears to be not entirely consistent and requires further investigation.
Although not as extensively studied as the metabolic interactions, the overall propensity
of recommended doses of GB products to alter the functionality of transporter proteins
appears low. Therapeutic and supra-therapeutic G. biloba doses (i.e., 240 and 360 mg/day) were shown to modestly increase the bioavailability
of the P-glycoprotein (ABCB1) probe substrates digoxin and talinolol by about 22 %
to 26 % [33], [34]. A study employing G. biloba treatment with 240 mg/day, however, did not show any alterations in the disposition
of fexofenadine, which is a substrate of P-glycoprotein (ABCB1), the intestinal uptake
transporters organic anion transporting polypeptide 1A2 (OATP1A2) and 2B1 (OATP2B1),
and the hepatic uptake transporters OATP1B1 [41], thereby indicating that GB is unlikely to alter the function of these transporter
proteins to a significant extent in vivo [21]. Similarly intermediate doses of GB (120 mg/day) do not appear to alter the function
of membrane monoamine transporters (PMAT), organic cation transporters (e.g., OCT1
and OCT3), and other polyspecific tubular OCT-transporters in the kidney (e.g., OCTN1,
OCTN2, MATE1, and MATE2-K), as shown by a recent metformin interaction study [37].
Milk thistle
Silybum marianum (family Asteraceae/Compositae), commonly known as milk thistle (MT), is one of the
oldest and most extensively studied plants in the treatment of alcoholic, toxic, and
viral liver diseases. Other treatment claims include prevention/amelioration of drug-induced
liver toxicity (DILI), e.g., due to cancer chemotherapy, nonalcoholic fatty liver
disease (NAFL), or steato hepatitis (NASH), lowering of cholesterol levels, and improvement
of insulin resistance in patients with type 2 diabetes (T2DM) [42]. The active extract of MT is denoted as silymarin and represents a mixture of the
flavonolignan constituents sylibin (silibinin), isosilybin, silidianin, and silichristin.
Silymarin is extracted from dried MT seeds, in which it is contained at higher concentrations
than in other parts of the plant. Silymarin has structural similarities to steroid
hormones, which may be linked to its pharmacological actions. Silybin is the predominant
and pharmacologically most active component, constituting approximately 60 % to 70 %
of the isomers, followed by silychristin (20 %), and silydianin (10 %) [43]. The silymarin content in MT extracts may range from 40 % to 80 % [43]. Customary daily doses of MT extract range between 210 to 800 mg, depending on patient
characteristics and therapeutic objectives.
In vitro studies implicate that silymarin and silybin can display inhibition of a variety
of CYP enzymes such as CYP3A4, CYP2C8, CYP2C9, CYP2C19, CYP2D6, or CYP2E1, and also
a couple of UGTs, such as UGT1A1, 1A6, 1A9, 2B7, and 2B15 [44], [45], [46], [47]. In contrast, there is no conclusive in vitro evidence that MT would have significant effects on the activity of P-gp (ABCB1) [45], [48].
The available evidence from in vitro studies, has been acknowledged by clinical researches, and a number of clinical studies
have been conducted for the in vivo assessment of the effects of MT products on various CYP isoforms, other drug metabolizing
enzymes, and transporters, predominantly by using specific probe drugs, thereby allowing
the identification and characterization of MT effects on specific metabolic and disposition
pathways. Altogether, we identified a total of 13 pharmacokinetic drug interactions
with orally administered MT extract in the public domain.
In the oldest published human MT interaction study, administration of silymarin at
the lower end of usually recommended daily doses (Legalon®, 70 mg t. i. d. = 210 mg/day)
for 28 days to 16 healthy subjects had no effect on the PK of aminopyrine or phenylbutazone,
two nonspecific CYP probes [49].
Gurley and colleagues studied 12 healthy subjects (6 m/6 f), who were randomly assigned
to receive MT (175 mg b. i. d. = 350 mg/day, standardized to 80 % silymarins) for
28 days [50]. Probe drug cocktails of midazolam (MDZ; CYP3A4) and caffeine (CYP1A2), followed
24 hours later by chlorzoxazone (CYP2E1) and debrisoquine (CYP2D6), were administered
before (baseline) and at the end of supplementation. Presupplementation and postsupplementation
assessments of CYP3A4, CYP1A2, CYP2E1, and CYP2D6 activities were made by using 1-hydroxymidazolam
(1-OH-MDZ)/MDZ serum ratios (1-hour sample), paraxanthine/caffeine serum ratios (6-hour
sample), 6-hydroxychlorzoxazone/chlorzoxazone serum ratios (2-hour sample), and debrisoquine
urinary recovery ratios (8-hour collection), respectively. The results of this study
indicate that daily doses of 350 mg of a standardized MT extract did not alter human
CYP1A2, CYP2D6, CYP2E1, and CYP3A4 enzyme activities in vivo and thus, do not appear to pose a significant interaction potential for substrates
of these enzymes [50].
In another study conducted by the same group of investigators, 19 healthy young subjects
(10 m/9 f) received substantially higher daily doses of a standardized MT supplement
for 14 days (900 mg/day). Subjects also received rifampin (300 mg b. i. d.) and clarithromycin
(500 mg b. i. d.) for 7 days as positive controls for CYP3A induction and inhibition,
respectively. Oral MDZ (8 mg) was administered as a CYP3A4 probe before and after
supplementation and control periods [51].
In contrast to the positive controls rifampin and clarithromycin, no significant changes
in MDZ PK were observed as a result of MT supplementation. Likewise, comparisons of
1-hour 1-OH-MDZ/MDZ ratios before and after supplementation/medication mirrored the
effects observed for MDZ concentration-time profiles, i.e., no significant changes
in mean 1-hour 1-OH-MDZ/MDZ serum ratios were noted after a 14-day course of MT (900 g/day),
thereby confirming that MT extract is not a clinically relevant modulator of CYP3A4
activity in vivo.
Likewise, coadministration of 560 mg silymarin (4 silymarin 140 mg capsules, Legalon®)
administered 10 hours and 1.5 hours prior to the administration of the CYP3A4 substrate
nifedipine (10 mg IR tablet) to 16 young healthy male subjects did not considerably
change the extent of absorption or metabolism of nifedipine. Overall, the total extent
of nifedipine exposure (i.e., AUC) was 13 % higher (90 % CI 0.97- to 1.32) in the
silymarin period, while Cmax values were on average 30 % lower (90 % CI 0.39- to 1.27), consistent with a trend
towards a delayed nifedipine absorption in the silymarin period [52]. On the whole, also this study confirmed that silymarin does not act as a potent
CYP3A4 inhibitor in vivo. The data suggest modest effects of silymarin on the absorption characteristics of
nifedipine, resembling the known pattern of nifedipine food effects. The authors discussed
that underlying mechanisms could be either a delay in the gastric emptying rate by
silymarin or inhibition of OATP transporters expressed in the GI tract [52].
The effects of a 14-day treatment with a 900-mg daily dose of a standardized MT extract
(300 mg t. i. d., standardized to contain 80 % silymarin per capsule) on the activity
of CYP2D6 were investigated in 16 healthy subjects (8 m/8 f), by administration of
the CYP2D6 substrate debrisoquine (5 mg) before and at the end of MT supplementation,
by using 8-h debrisoquine urinary recovery ratios [53]. Results did not indicate significant inhibition of CYP2D6 by MT supplementation
(post/pre geom. mean ratio 0.97, 90 % CI 0.93 to 1.10).
Two PK interaction studies, each of them conducted in 10 healthy male and female subjects,
examined if MT extract may have the potential to alter the PK of the HIV protease
inhibitor and CYP3A4 and P-gp substrate indinavir [54], [55].
In the first study, subjects received four oral doses of indinavir 800 mg every 8
hours for baseline PK, followed by a 3-week MT treatment (175 mg tablets t. i. d. = 525 mg/day;
each tablet containing 153 mg of silymarins, equivalent to 191 mg of MT extract standardized
for 80 % silymarins; Thisilyn). Then the course of four oral doses of indinavir 800 mg
every 8 hours was repeated on top of continued MT treatment, and results were compared
with baseline indinavir PK outcomes [55]. Indinavir plasma concentrations were followed for 8 h postdose. Results showed
that regular MT treatment over 3 weeks did not significantly alter the overall exposure
to indinavir, as evidenced by a nonsignificant average 9 % reduction in indinavir
+ MT AUC0–8 compared to indinavir alone treatment at baseline. On examination of individual patients,
the AUC0–8 of indinavir in the presence of MT was 70–107 % of the AUC0–8 for indinavir alone. Average indinavir trough values taken at 8 hours postdose, displayed
a statistical significant, albeit modest average decline of about 25 % upon MT treatment.
In the second indinavir/MT interaction study, indinavir 800 mg t. i. d. was given
for four doses, followed by MT 160 mg t. i. d. (= 480 mg/day standardized MT) for
13 days, with both drugs given at the same dosages over the following 2 days [54].
Indinavir plasma concentrations were followed up to 5 hours postdose. When given alone
and combined with MT, respectively, the geometric mean (95 % CIs) steady-state indinavir
AUC was 20.7 hr · mg/L (15.3–28.2 hr · mg/L) and 19.4 hr · mg/L (15.8–23.6 hr · mg/L),
and the trough plasma concentrations were 0.340 mg/L (0.232–0.497 mg/L) and 0.232 mg/L
(0.129–0.419 mg/L). Average observed maximum exposure (Cmax) was 8.85 (7.17–10.9) mg/L and 7.85 mg/L (6.55–9.30) for indinavir alone and indinavir
+ silymarin, respectively. None of the PK outcomes for indinavir indicated a statistical
significant alteration by MT treatment, and the authors concluded that a daily dosage
of 480 mg of a standardized silymarin extract has no apparent effect on indinavir
plasma concentrations. Taken together, both indinavir interaction studies yielded
consistent results, thereby indicating that MT supplementation at commonly recommended
doses does not alter the activity of CYP3A4 in vivo to a clinically relevant extent.
A study with another CYP3A4 and putative P-gp substrate, the H2 receptor antagonist ranitidine, also yielded a consistent outcome in that regard
[56]. In this trial, 12 healthy male subjects received oral ranitidine 150 mg either
alone or after a 7-day pretreatment with silymarin 140 mg t. i. d. (= 420 mg/day).
There was no influence of silymarin coadministration on the pharmacokinetics of ranitidine
(i.e., Cmax and AUC).
In a sample of 6 cancer patients, van Erp and colleagues investigated whether short-term
MT supplementation affects the PK of the anticancer drug irinotecan, a substrate for
CYP3A4 and UGT1A1 enzymes [57]. Patients were treated with irinotecan (dose 125 mg/m2) given as a 90-minute infusion once weekly. Four days before the second irinotecan
dose, patients received 200 mg MT capsules t. i. d. (= 600 mg/day), for 14 consecutive
days (each capsule containing 200 mg MT seed extract, containing 80 % silymarin).
Short-term (4 days) or more prolonged (12 days) intake of MT in this study had no
significant effect on irinotecan clearance (mean, 31.2 vs. 25.4 vs. 25.6 L/h; p = 0.16).
The AUC ratio of the active metabolite SN-38 and the irinotecan parent drug was slightly
decreased by MT (2.58 % vs. 2.23 % vs. 2.17 %; p = 0.047), whereas the relative extent
of glucuronidation of SN-38 was similar (10.8 vs. 13.5 vs. 13.1; p = 0.64). Likewise,
the AUC ratio of 7-ethyl-10-[4-N-(5-aminopentanoic acid)-1-piperidino]-carbonyloxycamptothecin
and irinotecan was unaffected by MT (0.332 vs. 0.285 vs. 0.337; p = 0.53). This study
showed that the concurrent use of MT does not significantly influence the PK of irinotecan
in cancer patients. The authors indicated that irinotecan is known to be highly susceptible
to CYP3A4 and P-gp (ABCB1) inhibition and induction in humans and concluded that the
results of the current study confirm that MT does not seem to modulate the activity
and/or expression of these proteins. They also noted that this finding is consistent
with the notion that the MT constituent silybin is not a ligand activator of the pregnane
X receptor (PXR), a steroid- and xenobiotic-regulated transcription factor that is
a key regulator of constitutive expression and induction of CYP3A4, UGT1A, and ABCB1.
The results of the irinotecan study also provide additional information in support
of an apparent lack of effect of MT on the function of human carboxylesterase 2–mediated
hydrolysis and UGT1A-mediated phase II conjugation pathways in humans.
There are two studies regarding the assessment of potential MT effects on the PK of
more or less sensitive CYP2C9 substrates available [58], [59]. Han and colleagues investigated the effects of silymarin on the PK of the angiotensin
II (ATII) receptor antagonist losartan (50 mg) and its active metabolite E-3174 and
its possible relationship to CYP2C9 genotypes because current evidence suggests that
losartan biotransformation to E-3174 is highly dependent on CYP2C9 activity [59]. Twelve healthy adult male subjects of known CYP2C9 genotype (six CYP2C9*1/*1 and
six CYP2C9*1/*3) were enrolled, and the PK of losartan and E-3174 were assessed before
and after a 14-day treatment with 140 mg of silymarin t. i. d. (= 420 mg/day; Legalon®
140 mg capsules).
About ≥ 2-fold differences in the AUC0–24 (409.9 ± 76.9 vs. 854.3 ± 236.9 ng h/mL, placebo vs. silymarin, p < 0.05), AUC0-∞ (425.0 ± 83.9 vs. 886.0 ± 221.1 ng h/mL, placebo vs. silymarin, p < 0.05) and Cmax (170.9 ± 54.6 vs. 323.3 ± 121.0 ng/mL, placebo vs. silymarin, p < 0.05) of losartan
between the placebo and silymarin treatments were observed in carriers of CYP2C9*1/*1.
Accordingly, the oral clearance (CL/F) of losartan was also significantly decreased
by more than 2-fold after a 14-day silymarin treatment in these subjects (121.9 ± 27.2
vs. 59.7 ± 159.7 L/h, placebo vs. silymarin, p < 0.05). In contrast, in carriers of
CYP2C9*1/*3, no significant differences in any of losartan PK parameters between the
placebo and silymarin treatment groups were observed, e.g., AUC0–24 was 816.3 ± 282.9 vs. 805.8 ± 266.2 ng h/mL, and AUC0-∞ was 866.0 ± 292.8 vs. 871.9 ± 23 ng h/mL. Likewise, the metabolic ratio of losartan
(ratio of AUC0-∞ of E-3174 to AUC0-∞ of losartan) was significantly decreased after silymarin treatment in carriers of
CYP2C9*1/*1 (p < 0.05), but not in those with the CYP2C9*1/*3 genotype (p = 0.065).
Notably, the total exposure parameters (i.e., AUC0–24 and AUC0-∞) in placebo-treated carriers of CYP2C9*1/*3 were well comparable to silymarin-treated
CYP2C9*1/*1 subjects, thereby suggesting that the extensive CYP2C9 metabolizers (i.e.,
carriers of CYP2C9*1/*1 wildtype) were actually subject to a silymarin-mediated pharmacological
conversion to heterozygous intermediate CYP2C9 metabolizers (i.e., CYP2C9*1/*3).
As CYP2C9 appears responsible for oxidative conversion of losartan to its pharmacologically
active carboxylic acid metabolite E-3174, the data of the present study are consistent
with a substantial CYP2C9 inhibitory potential of silymarin treatment at customary
doses in vivo. As E-3174 has a higher ATII antagonistic potency and longer half-life than the parent
compound losartan, the active metabolite may be possibly responsible for most of the
antihypertensive effect, and the decrease in the metabolic ratio of losartan to E-3174
due to decreased CYP2C9 activity may diminish the efficacy of losartan in carriers
of CYP2C9*1/*1 [59]. The study results of Han et al. were recently followed up by Brantley and colleagues,
who identified in vitro that silybin A and silybin B were the most potent CYP2C9 inhibiting constituents
of silymarin extract, with Ki values of 4.8 and 10 µM, respectively [44]. In mechanistic terms, the available CYP2C9-related evidence suggests that MT treatment
may have a potential to inhibit the clearance and to increase the exposure of other
sensitive CYP2C9 substrates such as (S)-warfarin in vivo.
An earlier study examined the influence of a 9-day silymarin pretreatment at a low
daily dose of 140 mg on the metabolism and disposition of metronidazole (3 × 400 mg/day),
a substrate of CYP3A4 and CYP2C9, in 12 healthy adult subjects [58]. In this study, low-dose silymarin modestly increased the clearance of metronidazole
and its major metabolite, hydroxy-metronidazole (HM), by 29.51 % and 31.90 %, respectively,
with a concomitant decrease in half-life, Cmax, and AUC0–48. However, urinary excretions of acid-metronidazole (AM), HM as well as metronidazole
in 48 h were decreased. The authors concluded that the results indicate that silymarin
might induce both intestinal P-gp and CYP3A4 upon multiple dose administration. However,
this interpretation would not be consistent with all other published in vivo studies examining the effects of silymarin on CYP3A4 and P-gp function. Hence, the
underlying mechanism(s) of the reported modest silymarin/metronidazole PK interaction
remain(s) to be determined.
Two studies investigated the effect of MT administration on the PK of the prototypic
P-gp (ABCB1) substrates digoxin [60] and the β-adrenoceptor antagonist talinolol [61], with the aim to examine potential in vivo effects of MT treatment on P-gp function.
Sixteen healthy young subjects (8 m/8 f) were randomly assigned to receive a standardized
MT supplement for 14 days (300 mg t. i. d. = 900 mg/day, standardized to contain 80 %
silymarin), or to receive rifampin (300 mg b. i. d. = 600 mg/day, 7 days) and clarithromycin
(500 mg b. i. d. = 1000 mg/day, 7 days) as positive controls for P-gp (ABCB1) induction
and inhibition, respectively. Oral digoxin (Lanoxicaps™, 0.4 mg) was administered
as a P-gp probe before and at the end of supplementation and control periods. Serial
digoxin serum concentrations were obtained over 24 h [60].
As expected, rifampin produced significant reductions (p < 0.01) in measures of total
(AUC0–3, AUC0–24) and maximum (Cmax) digoxin exposure, whereas clarithromycin increased these parameters significantly
(p < 0.01). In turn, no remarkable effects on digoxin PK were observed following supplementation
with MT, although digoxin AUC0–3 and AUC0–24, and Cmax were altogether found modestly reduced following MT administration by about 9.5 %,
12.5 %, and 13.5 %, respectively. These results do not support reported in vitro data suggesting a potential inhibition of P-gp function by MT constituents and also
do not suggest a clinically relevant modulation of P-gp activity by MT supplementation
at customary doses.
Han and coworkers examined the effect of concomitantly administered silymarin on the
pharmacokinetics of talinolol, a drug mainly eliminated into the intestine by P-gp,
in 18 healthy male Chinese subjects, and its association with a genetic single nucleotide
polymorphism in exon 26 (C3435T) of the MDR-1 (ABCB1) gene [61], which was shown to be correlated with intestinal expression and in vivo activity of P-gp [62]. The PK of talinolol was assessed after coadministration of either placebo or 140 mg
silymarin capsules t. i. d. (420 mg/day) for 14 days, and talinolol plasma concentrations
were followed for 36 hours. Talinolol peak plasma concentrations (Cmax) were significantly increased after silymarin administration as compared with placebo
(p = 0.007), and the total exposure estimates (AUC0–36 and AUC0–∞) of talinolol were increased on average by about 36 % by silymarin coadministration.
Accordingly, the oral clearance (CL/F) of talinolol was decreased by about 23 % (p < 0.001).
These effects were unrelated to the MDR-1 C3435T single nucleotide polymorphism, thereby
suggesting a P-gp-unrelated mechanism of this finding, probably consistent with an
upregulation of intestinal organic anion transporting polypeptide (OATP) mediated
uptake of talinolol [63].
Deng and coworkers examined the interaction of MT extract and the cholesterol lowering
HMG-CoA-reductase inhibitor rosuvastatin [64]. Eight healthy Korean male subjects were either treated with placebo or silymarin
(Legalon® 140 mg capsules) t. i. d. (= 420 mg/day) for 5 days. Subjects received a
single 10 mg dose of rosuvastatin on day 4 of silymarin supplementation, and serial
blood samples were collected for 72 h. During plasma sampling, subjects continued
to take silymarin or placebo capsules t. i. d. for 2 further days. The plasma concentration–time
profile of rosuvastatin showed no significant effect of silymarin treatment. Cmax values (mean ± SD) for rosuvastatin were 13.7 ± 4.7 ng/mL and 14.8 ± 5.3 ng/mL in
the presence and absence of silymarin, respectively. The AUC values (mean ± SD) for
rosuvastatin with and without silymarin treatment were 134.6 ± 42.3 and 144.0 ± 38.7 ng
h/mL, respectively. Thus, short-term silymarin treatment at customary doses did not
significantly affect the PK of rosuvastatin, a multitransporter substrate, including
the intestinal efflux/uptake transporters P-gp (ABCB1), BCRP (ABCG2), and OATP1A2,
and the hepatic uptake transporters OATP1B1, 1B3, and 2B1 [65], indicating that silymarin is not a potent modulator of ABC and OATP transporters
in vivo.
In conclusion, the available evidence from a total of 13 clinical PK interaction studies
consistently indicates that marketed MT/silymarin products, given at customary or
supra-therapeutic doses, do not alter the function of CYP3A4, CYP1A2, CYP2D6, and
CYP2E1 enzymes, or intestinal ABC and OATP transporters to a relevant extent, although
the findings regarding a potential modulation of the activity of certain OATP transporters
is not sufficiently detailed and entirely conclusive as yet, and deserves further
investigation. Due to the total number of studies available and the high degree of
consistency in overall study outcomes, the evidence in terms of a lack of interaction
potential is most robust for CYP3A4 and P-gp (ABCB1). The results from a single study
in irinotecan-treated cancer patients also suggest that the function of UGT1A1 and
carboxylesterase 2 are unlikely to be substantially altered by MT/silymarin.
In contrast, the available evidence from one well-designed study in Chinese subjects
regarding CYP2C9 [59], together with consistent in vitro data [44], strongly suggests the possibility of significant CYP2C9 inhibition by MT/silymarin.
These data call for further investigation and confirmation of the clinical significance
of this matter with narrow therapeutic index CYP2C9 substrates such as warfarin.
Ginseng
Several ginseng species (family Araliaceae) need to be distinguished including Panax ginseng (Asian ginseng), Panax quinquefolius (American ginseng), and Siberian ginseng (Eleutherococcus senticosus Maxim). For centuries ginseng has been used as a traditional herbal medicine in many
Asian countries. Today ginseng products are consumed worldwide and rank among the
most commonly used herbal products. Actually, ginseng is the 5th best-selling herb
in the United States [66]. Ginseng products are advocated for many conditions, including maintenance of general
health, physical and mental well-being, treatment of fatigue, weakness and mild depression,
improving immune function, and for conferring antioxidant and antineoplastic pharmacological
effects [67], [68].
Among the different principal ginseng constituents, which are derived from the dried
roots of the plants, the major active components are ginsenosides, which represent
a diverse group of steroidal saponins [68]. Some remarkable differences between ginseng species exist regarding the content
and composition of ginsenosides, a factor that may be associated with a differential
drug interaction potential. The recommended daily dose of P. ginseng is 200 mg of standardized (i.e., 4 % total ginsenosides) extract.
The effects of ginseng extracts or individual ginsenosides on CYP enzymes have been
examined in many published in vitro studies. Unfortunately the results have been largely inconsistent and remain controversial.
Also the concentrations required to elicit CYP inhibition or induction in vitro were often very high and unlikely to be achieved in vivo with recommended doses of marketed products [69]. Hence, reliable predictions on the in vivo relevance of these findings are hardly possible.
Clinical studies on PK ginseng-drug interactions are scarce; we identified a total
of 9 published PK interaction studies reporting original data.
In a phenotyping study measuring single timepoint, phenotypic metabolic ratios in
elderly subjects with administration of the prototypic enzyme-specific probe drugs
MDZ (CYP3A4), caffeine (CYP1A2), chlorzoxazone (CYP2E1), and debrisoquine (CYP2D6),
the potential of P. ginseng, at a dose of 500 mg t. i. d. (= 1.500 g/day, standardized to 5 % ginsenosides) given
for 28 days, to alter the activities of these CYP enzymes was investigated [18]. In this setting, P. ginseng did not alter the function of CYP3A4, CYP1A2, and CYP2E1, and displayed only a marginal
reduction of CYP2D6 activity (~ 7 %), an effect magnitude not being of clinical concern.
In a similar study in healthy young subjects, using the same P. ginseng product, dose, and treatment duration, as well as the same CYP probe drugs, no significant
effects of P. ginseng on any CYP enzyme activity were found [17].
The findings of Gurley et al. in terms of unaltered CYP3A4 activity were confirmed
by another study, in which P. ginseng 100 mg doses (Ginsana™, standardized to 4 % ginsenosides) were given b. i. d. (= 200 mg/day)
for 14 days to 20 healthy male and female subjects (10 Caucasians, 10 Asians). In
this study, the urinary 6-β-hydroxycortisol/cortisol ratio, a marker of CYP3A enzyme induction, remained unaltered,
thereby indicating that P. ginseng, at recommended doses, does not confer significant CYP3A induction in vivo [70].
The effects of Siberian ginseng (SG; Eleutherococcus senticosus Maxim; 485-mg capsules of standardized extract twice daily) on the activity of CYP2D6
and 3A4 were assessed in a 2-week study in 12 healthy male and female subjects [71]. Dextromethorphan (CYP2D6) and alprazolam (CYP3A4) were used as probe drugs. There
were no statistically significant differences between pre- and post-SG treatment urinary
dextromethorphan/dextrorphan metabolic ratios, indicating a lack of an SG effect on
CYP2D6 enzyme activity. For alprazolam there also were no significant differences
in plasma concentrations and derived PK parameters (Cmax, tmax, AUC, t1/2) indicating that SG does not significantly induce or inhibit CYP3A4. These results
are consistent with the outcomes reported for P. ginseng preparations as detailed above and indicate that standardized extracts of SG at generally
recommended doses are unlikely to alter the disposition of coadministered medications
primarily dependent on the CYP2D6 or CYP3A4 pathways for their elimination [71].
The effects of American ginseng (AG; Panax quinquefolius) on the disposition of the HIV protease inhibitor and sensitive CYP3A4 substrate
indinavir were assessed in 14 healthy adult male and female subjects (12 African-American
and two Caucasians) [72]. Indinavir at 800 mg doses was administered t. i. d., either alone or together with
1.0 g doses of AG three times daily over 2 weeks (dried whole root 500 mg capsules).
The study demonstrated that AG did not alter the PK of the CYP3A4 substrate indinavir,
thereby demonstrating the absence of appreciable CYP3A4 induction or inhibition.
Lee and colleagues assessed the effects of a 2-week treatment of 200 mg b. i. d. (= 400 mg/day)
enriched AG extract (HT1001 100 mg capsules; REMEMBER-fX; total ginsenoside content
of 8.5 ± 0.5 %) on the PK profile of the nucleoside reverse transcriptase inhibitor
zidovudine (single 300-mg oral doses of Retrovir™) [73]. This study provided the first in vivo insights into the potential effects of AG on phase 2 drug metabolizing enzymes, as
zidovudine is predominantly cleared through glucuronidation by UGT 2B7. The study
results showed that AG does not significantly affect the formation clearance of zidovudine
to its glucuronide (ratio post- to pre-AG = 1.17; 90 % confidence interval: 0.95–1.45;
p = 0.21), total clearance (ratio = 0.97; 0.82–1.14; p = 0.70), or total plasma zidovudine
exposure (AUC0–8 ratio = 1.03; 0.87–1.21; p = 0.77).
Jiang and colleagues investigated the effects of P. ginseng on the PK and PD of warfarin in 12 healthy male subjects (eight Caucasians, four
Asians) [74]. A single 25-mg dose of warfarin (Coumadin™, 5 × 5-mg tablets) was administered
to each subject with and without pretreatment with multiple doses of ginseng for 1
week (Golden Glow = Korean ginseng, each capsule containing extract equivalent to
0.5 g Panax ginseng root and 8.93 mg ginsenosides as ginsenoside Rg1; 2 × 0.5-g capsules, t. i. d. = 3 g).
Dosing of ginseng was continued for a further 1 week after warfarin administration.
The bioanalytics comprised enantiomer-selective quantification of S-warfarin, which
is predominantly metabolized to S-7-hydroxywarfarin by CYP2C9, and R-warfarin, which
is metabolized by CYP3A4 and CYP1A2, thereby allowing for a separate mechanistic assessment
of any potential alteration of these metabolic pathways by concomitant P. ginseng treatment. The study demonstrated that P. ginseng had no effect on the activity of CYP1A2, CYP3A4, or CYP2C9 in healthy subjects, as
treatment did not affect the PK and clearance of both warfarin enantiomers in human
subjects. P. ginseng also did not affect the apparent volumes of distribution or protein binding of warfarin
enantiomers. PD endpoints of warfarin 25 mg single doses were also assessed in this
study, and it was shown that P. ginseng did not significantly alter blood coagulation (i.e., INR) outcomes and platelet aggregation.
The results of this study were essentially confirmed by the same group of investigators
in a warfarin interaction study of similar design, and in a comparable population
(12 healthy male subjects, 6 Caucasian, 6 Asian), but using a different product (Blackmores
Travel Calm Ginger, Zingiber officinale, each capsule containing extract equivalent to 0.4 g of ginger rhizome powder) at
different daily doses (3 capsules, 3 × daily for 1 week) [26]. Similar as in the previous study, dosing of the herbal product was continued for
a further week after warfarin administration. Also in this study, the PK and PD of
warfarin were essentially unaltered by repeat-dose coadministration of recommended
ginseng doses, thereby confirming the findings of the previous study [26]. Finally, the results of both studies [27], [75] were pooled and subjected to a population PK/PD modeling approach [74]. The pooled data analyses indicated that the ratio of S-warfarinʼs apparent clearance
(CL/F) compared to control (= ratio warfarin + ginseng/warfarin alone) was 1.14 ± 0.04
after P. ginseng pretreatment, indicating that P. ginseng produced only a moderate increase in the CL/F of S-warfarin. None of the two ginseng
products employed in the pooled studies had a direct effect on warfarin PD [74].
In another randomized, double-blind, placebo-controlled parallel group study in healthy
adult subjects, the effects of AG (P. quinquefolius, 0.5 g capsules, total ginsenoside content of 5.19 %) on the PK and PD of warfarin
were studied [76]. Patients received oral warfarin (Coumadin, DuPont Pharmaceuticals), 5 mg daily,
for the first 3 consecutive days during week 1. Beginning in week 2, patients were
randomly assigned to receive either oral AG, 1.0 g, or placebo b. i. d. (= 2 g/day),
for 3 consecutive weeks. During week 4, all patients again received oral warfarin,
5 mg daily, for the first 3 consecutive days. Ginseng or placebo assignment was randomized,
and a total of 20 subjects were enrolled (9 m/11 f; 12 subjects in the ginseng group,
8 subjects in the placebo group). The authors compared changes in peak INR, INR AUC,
peak plasma warfarin levels, and warfarin AUCs between the ginseng and placebo groups.
The peak INR was shown to decrease statistically significantly after 2 weeks of ginseng
administration compared with placebo (difference between ginseng and placebo, − 0.19
[95 % CI, − 0.36 to − 0.07]; p = 0.0012). The INR area under the curve (AUC), peak
plasma warfarin level, and warfarin AUC were also significantly reduced in the ginseng
group as compared with the placebo group. Also, peak INR and peak plasma warfarin
levels were positively correlated. The INR responses in the ginseng group were modest,
albeit significantly different from the placebo group. The average group results,
however, have to be interpreted with caution, as the INR data in the ginseng group
were largely driven by one outlier patient displaying a high baseline INR (1.32) and
a remarkably increased peak INR after warfarin administration on day 4. After ginseng
administration, the peak INR of this patient declined remarkably from 5.16 to 2.75,
and the corresponding AUCINR decreased from 17.46 to 11.1. Although the authors emphasize that the results of
their study remain statistically significant, when this patient is excluded from analysis,
it becomes evident, that parallel group studies of such a small sample size are difficult
to interpret.
Taken together, the available published evidence from clinical ginseng/warfarin interaction
studies indicate that high repeated doses of P. ginseng and ginger over 1 week did not significantly alter the PK or PD of 25 mg warfarin
single doses in healthy adult subjects [27], [74], [75]. In contrast, a small parallel group study in healthy subjects using a different
warfarin posology (5 mg warfarin q. d. on 3 consecutive days) and a 2-week treatment
with AG (P. quinquefolius) at the high end of the recommended dose range suggests that AG may reduce warfarin
exposure (i.e., increases warfarin clearance), thereby reducing the anticoagulant
effect of warfarin to a modest extent. The differences in the outcome of these studies
might be either due to differential properties of P. ginseng and AG (P. quinquefolius) products, differences in the study designs (1-week vs. 2-week treatments), or simply
due to the limitations of the small parallel group study which was apparently confounded
by an outlier subject [76].
While all ginseng/warfarin interaction studies available so far suffer the principle
design limitation of short-term warfarin treatments in healthy adult subject populations,
which may not be predictive for long-term warfarin treatment in typical patient populations,
the totality and consistency of data derived from mechanistic PK studies as detailed
above, strongly suggest a lack of relevant CYP enzyme inhibitory or induction properties
of ginseng products at recommended therapeutic doses.
As yet, no clinical studies on the investigation of ginseng products regarding their
capacity to confer transporter-based interactions have been published. Therefore,
the available published evidence from clinical trials suggests an overall low potential
of virtually all ginseng species for CYP-based ginseng-drug interactions, while clinical
studies on transporter-based interactions are still lacking.
Goldenseal
Goldenseal (Hydrastis canadensis, orangeroot) is a perennial herb in the buttercup family Ranunculaceae, indigenous
to southeastern Canada and the northeastern United States. Botanical supplement goldenseal
(GS) preparations are taken for the prevention and treatment of the common cold, upper
respiratory and gastrointestinal infections, as well as menstrual disorders. In addition,
GS is being used as a topical antimicrobial remedy to disinfect cuts and scrapes.
GS contains a number of isoquinoline alkaloids such as hydrastine, berberine, berberastine,
hydrastinine, tetrahydroberberastine, canadine, and canalidine. Hydrastine and berberine
are the main active constituents [77].
As a supplement, standardized extracts of GS are available in liquid, tablet, and
capsule forms. Recommended doses of the powdered root vary widely and range from 750 mg
to 6 grams in tablet or capsule form per day, to be given 2 to 3 times daily. GS preparations
enjoy widespread popularity and are among the top 20 bestselling botanical products
in the US [78].
A total of 8 published pharmacokinetic in vivo drug interactions with orally administered GS root extract (5 studies) or berberine
products (3 studies) were identified.
In the first published study, the influence of GS root extract (GS root herbal single
570-mg capsules, two capsules b. i. d. = 2.280 mg/day, given for 14 days) on the disposition
of the HIV protease inhibitor as well as CYP3A4 and P-gp substrate indinavir was examined
in 11 healthy subjects (6 m/5 f) [79]. Indinavir was given as a single 800-mg oral dose; blood samples were collected
for 8 hours postdose, and the PK of indinavir was characterized before and after 14
days of GS treatment. No statistically significant alterations in the PK of indinavir
were observed (AUC values not reported). The oral clearance (CL/F) of indinavir was
lowered on average by 4.6 % following GS treatment, with individual changes ranging
from − 46.1 % (subject 5) to a 46 % increase (subject 4), indicating a remarkable
intersubject variability in this study. Accordingly, the 95 % CI for the difference
in oral clearance between treatments was large (− 10.6 to 3.4 L/h). Similar changes
were observed in Cmax, with the 95 % CI for the difference in peak concentration with goldenseal root ranging
from − 0.6 to 2.5 mg/L. Overall, the study results suggest that GS supplementation
at customary doses appears unlikely to affect the activity of CYP3A4 and/or P-gp (ABCB1)
to a relevant extent in vivo, although the remarkable intersubject variability observed may pose questions regarding
the robustness and reliability of the study. The authors also discussed the possibility
of a limited study sensitivity regarding inhibition of presystemic CYP3A4, as indinavir
was previously shown in a grapefruit juice interaction study to be a poor probe substrate
for assessing CYP3A4-mediated gut wall extraction [80].
In a subsequent study, single timepoint, metabolic ratios of CYP probe drugs were
used in 12 healthy subjects (6 m/6 f, extensive metabolizers of CYP2D6) to determine
whether a 28 day supplementation of GS root extract (900 mg t. i. d. = 2.700 mg/day;
no standardization claim) affects CYP1A2, CYP2D6, CYP2E1, or CYP3A4/5 activity [8]. Probe drug cocktails of MDZ (CYP3A4) and caffeine (CYP1A2), followed 24 hours later
by chlorzoxazone (CYP2E1) and debrisoquine (CYP2D6) were administered before (baseline)
and at the end of supplementation. Presupplementation and postsupplementation assessments
of CYP3A4, CYP1A2, CYP2E1, and CYP2D6 activities were made by use of 1-OH-MDZ/MDZ
serum ratios (1-hour sample), paraxanthine/caffeine serum ratios (6-hour sample),
6-hydroxychlorzoxazone/chlorzoxazone serum ratios (2-hour sample), and debrisoquine
urinary recovery ratios (8-hour collection), respectively.
Comparisons of pre- and postsupplementation metabolic ratios indicated significant
inhibition of about 40 % of CYP2D6 [geom. mean post-/pre-ratio (90 % CI) of 0.60 (0.51
to 0.70)] and CYP3A4/5 [geom. mean post/pre ratios (90 % CI) of 0.60 (0.53 to 0.67)]
activities due to GS supplementation, while there was no appreciable effect on CYP1A2
and CYP2E1 activities. Hence, this was the first clinical study strongly suggesting
that GS root extract, given at recommended doses, appeared to inhibit CYP2D6 and CYP3A4/5
to a notable extent in vivo.
For CYP3A4/5, the same group of authors confirmed this first clinical indication by
a more comprehensive PK interaction study that comprised the assessment of complete
plasma concentration-time profiles of the respective probe drugs [9]. In this study, the effects of GS supplementation (GS root extract 1.323 mg t. i. d. = 3.969 mg/day,
standardized to contain 24.1 mg isoquinoline alkaloids per capsule) on human CYP3A
activity were evaluated using MDZ as a phenotypic probe. Sixteen healthy young subjects
(8 m/8 f) were assigned to receive goldenseal for 14 days. Oral MDZ (8 mg) was administered
before and after goldenseal supplementation, and serum concentrations of MDZ and the
main metabolite 1-OH-MDZ were followed up to 6 hours postdose. In order to demonstrate
the sensitivity of the study to adequately capture any relevant effects on CYP3A4
activity, the well-established CYP3A4 inducing/inhibiting probe drugs rifampin (300 mg
b. i. d. for 7 days) and clarithromycin (500 mg b. i. d. daily for 7 days) were employed
in the study as well. Comparisons of pre- and post-GS supplementation MDZ PK parameters
indicated significant inhibition of CYP3A by GS (AUC0–∞ 107.9 ± 43.3 vs. 175.3 ± 74.8 ng · h/mL; Cl/F/kg, 1.26 ± 0.59 vs. 0.81 ± 0.45 l/h/kg;
T1/2 2.01 ± 0.42 vs. 3.15 ± 1.12 h; Cmax 50.6 ± 26.9 vs. 71.2 ± 50.5 ng/mL). Comparisons of these data show that mean MDZ
AUC0–∞ and Cmax data were increased by about 63 % and 40 %, respectively, upon GS supplementation.
In contrast, the potent CYP3A4 inhibitor clarithromycin produced about 5.5-fold (i.e.,
548 %) and about 2-fold (217 %) increases in MDZ AUC0–∞ and Cmax outcomes, respectively. Taken together, these data categorize GS root extract, at
the given dose, as a weak inhibitor of CYP3A4 (i.e., < 2-fold increase in AUC of the
sensitive CYP3A4 probe MDZ), according to current FDA drug interaction guideline recommendations
[81].
In a follow-up study addressing GS potential to inhibit CYP2D6, the effects of a 14-day
treatment with standardized GS root extract (1.070 mg, standardized to contain 24.1 mg
isoquinoline alkaloids per capsule, t. i. d. = 3.210 mg/day) on the activity of CYP2D6
was investigated in 16 healthy subjects (8 m/8 f), after administration of the CYP2D6
substrate debrisoquine (5 mg) before and at the end of supplementation, by using 8-h
debrisoquine urinary recovery ratios [53]. Comparisons of pre- and postsupplementation ratios indicated a significant inhibition
of CYP2D6 activity by GS supplementation by about 47 % (post/pre geom. mean ratio
0.53, 90 % CI 0.44 to 0.64), thereby confirming previously reported single-point phenotyping
data [18]. Quantitatively, these data categorize GS root extract, at the given dose, as a
weak inhibitor of CYP2D6 (i.e., < 50 % decrease in substrate clearance) according
to current FDA drug interaction guideline recommendations.
To examine potential effects of GS root extract supplementation on P-gp (ABCB1) function
in vivo, 20 healthy young subjects (10 m/10 f) were randomly assigned to receive a standardized
GS supplement (1.070 mg t. i. d. = 3.210 mg/day) for 14 days or to receive the established
positive control probe drugs rifampin (300 mg b. i. d. for 7 days) and clarithromycin
(500 mg b. i. d. for 7 days). Supplementation/medication phases were separated by
30-day washout periods. Digoxin (Lanoxin™, 0.5 mg) was administered p. o. before and
at the end of each supplementation/medication period, and serial digoxin serum concentrations
were determined over 24 h postdose [82].
Statistically significant increases (p < 0.001) in digoxin total and maximum exposure
parameters [AUC0–24 (57 %), AUC0–3 (83 %), Cmax (95 %)], and terminal disposition half-life (79 %) were observed after 7 days of
clarithromycin treatment. Accordingly, clarithromycin produced a 53 % decrease in
CL/F (p < 0.001). In turn, statistically significant reductions (p < 0.05) in digoxin
AUC0–24 (− 16 %), AUC0–3 (− 27 %), and Cmax (− 28 %) were noted following rifampin administration. Taken together, the results
of the P-gp probe inhibitor/inducer indicate adequate sensitivity of the study to
accurately capture relevant effects on P-gp function. As a result of GS supplementation,
there were no significant changes in digoxin PK observed, apart from a 14 % increase
in digoxin Cmax (p < 0.05). Based on the results of this study, it can be concluded that GS root
extract supplementation at daily doses of about 3 g does not modify P-gp function
to a clinically relevant extent.
Two studies examined the effect of berberine, a major alkaloid constituent in GS extract,
on the PK of cyclosporine A (CsA) in Chinese renal transplant patients [83] and in healthy adult Chinese subjects [84].
In a randomized, controlled clinical trial in 104 renal transplant recipients, Wu
and colleagues examined the effects of oral berberine supplementation [BBR hydrochloride
tablets 200 mg (unspecified source), t. i. d. for 3 months] on the PK of CsA [83]. Patients were randomly assigned in a 1 : 1 fashion to a BBR-free group or to the
BBR treatment group. Blood trough concentration of CsA and markers for hepatic and
renal functions were determined. For a more comprehensive PK substudy, six renal transplant
recipients were treated with a 3-mg/kg dose of CsA b. i. d. before and after oral
coadministration of 200 mg BBR t. i. d. for 12 days.
In the clinical study, the final CsA trough concentrations and the ratios of concentration/dose
of CsA in BBR-treated patients were modestly increased, i.e., 29.3 % and 27.8 % higher
than those in BBR-free patients, respectively (p < 0.05). In the dedicated PK study
in 6 patients, the average increase in the total CsA exposure (AUC) upon BBR treatment
was 34.5 % (p < 0.05), while average increases in Css and Cmin values of CsA were 34.5 % and 88.3 %, respectively, without significant alterations
in Cmax. The average decrease in CL/F was 40.4 %. Overall, the study results suggest a weak
inhibition of CYP3A4 by BBR supplementation at a 600 mg/day dose level.
In the second BBR/CsA interaction study, the effects of 300 mg BBR, b. i. d. (= 600 mg/day),
given for 10 days on the pharmacokinetics of CsA were examined in 12 healthy male
Chinese subjects. CsA PK assessments at a dose of 6 mg/kg were done in 6 subjects
both before and at the end of the BBR treatment period, whereby the second CsA dose
was given ≥ 12 h after the last BBR dose. Another 6 subjects received 3 mg CsA/kg,
the second CsA dose being given concomitantly with a single oral dose of 300 mg BBR.
In the study with 6 mg CsA/kg treatment and time-separated intake of the last BBR
dose, BBR did not significantly alter the PK of CsA. However, in the trial with 3 mg
CsA/kg and the concomitant administration of CsA and BBR, a modest average increase
in the AUC of CsA of 19.2 % (p < 0.05) was observed, without any accompanying changes
in the terminal disposition half-life or apparent oral clearance (CL/F) of CsA. These
results indicate that BBR can modestly increase exposure of CsA, when both drugs are
administered together [84].
Taken together, although not entirely consistently, both BBR/CsA studies are suggestive
for a modest BBR-mediated inhibition of CYP3A4, which may need to be considered in
case of concomitant treatment with narrow therapeutic index drugs that are sensitive
substrates of CYP3A4, such as CsA.
In the most recent study, 18 Chinese healthy male adult subjects received orally either
placebo or berberine (BBR) capsules at a dose of 300 mg t. i. d. (= 900 mg/day) for
14 days, and phenotypic effects of BBR supplementation on the activities of CYP1A2,
CYP2C9, CYP2C19, CYP2D6, and CYP3A4 were examined by the administration of the probe
substances caffeine (93 mg), losartan (30 g), omeprazole (20 mg), dextromethorphan
(30 mg), and MDZ (2 mg), respectively [10]. The PK profiles of omeprazole, MDZ, and caffeine were assessed over 12 h postdose,
and the losartan/E-3174 as well as dextromethorphan/dextrorphan urinary ratios were
assessed by urine sampling over 8 h postdose.
A remarkable decrease in CYP2D6 activity was observed in this study as the 0–8 h urinary
dextromethorphan/dextrorphan ratio increased about 9-fold (p < 0.01). In addition,
the losartan/E-3174 ratio doubled (p < 0.01) after BBR administration, indicating
a notable decrease in CYP2C9 activity. In contrast, CYP3A4 activity was only modestly
inhibited, as the Cmax, AUC0–∞, and AUC0–12 of MDZ were increased 38 % (p < 0.05), 40 % (p < 0.01), and 37 % (p < 0.05), respectively,
after BBR treatment. Accordingly, the oral MDZ clearance was also modestly decreased
by 27 % (p < 0.05). There were no significant alterations of the probe substances
caffeine (CYP1A2) and omeprazole (CYP2C19). Taken together, repeated BBR administration
(900 mg/day) decreases CYP2D6 activity significantly, appears to modestly modify 2C9
and CYP3A4 activities, and does not alter CYP1A2 and CYP2C19 function.
In conclusion, 5 pharmacokinetic interaction studies with different GS root extract
products at daily doses up to 4 g are overall largely consistent and characterize
GS root extract as a weak inhibitor of CYP3A4 and CYP2D6 enzymes, when FDA drug interaction
guideline criteria are considered. The available evidence for this assessment is most
robust for CYP3A4, and based on phenotypic serum ratios or metabolite urinary recovery
data only, for CYP2D6. Results from single studies indicate that GS root extract does
not alter the activities of CYP1A2 and CYP2E1 enzymes, and the efflux transporter
P-gp (ABCB1) to an appreciable extent. No information is available regarding potential
effects of GS on CYP2C9 and CYP2C19 enzymes.
The available in vivo evidence on the PK drug interaction potential of BBR products is derived from 3 studies,
altogether conducted in Chinese subjects. These studies consistently point to a weak
potential of BBR products to inhibit CYP3A4. In addition, a single phenotyping study
suggests that BBR decreases CYP2D6 activity and modestly modifies CYP2C9 function,
but is unlikely to alter CYP1A2 and CYP2C19 functions. These findings and their clinical
relevance, however, need to be confirmed by future studies. So far, caution should
be applied when GS root extract or BBR products are coadministered with narrow therapeutic
index drugs, which are sensitive substrates of CYP3A4, CYP2D6 or CYP2C9 enzymes.
Garlic
Garlic (Allium sativum; family Amaryllidaceae) has been cultivated and used for the alleviation of a variety
of medical conditions for thousands of years. Today, garlic is worldwide still one
of the most popular and most commonly used herbal supplements with purported antioxidative,
antibacterial, antiparasitic, antilipidemic, antihypertensive, antiatherosclerotic,
antithrombotic, antidiabetic, immune-stimulating and anticancer properties [85], [86], [87].
Pharmaceutical products derived from garlic bulbs/cloves are provided in the forms
of powders, oily preparations, or aqueous-alcoholic extracts of fresh or aged garlic.
Due to the complex chemistry, differing manufacturing processes result in preparations
of quite different chemical composition and pharmaceutical properties, which in turn
may result in different pharmacological, safety, and bioavailability characteristics
[12], [88].
The so far identified active constituents of garlic include alliin, allinase, diallyldisulfide,
ajoens, and others. Alliin is enzymatically converted to allicin, the major garlic
component, which, however, displays considerable chemical instability and is rapidly
degraded to a variety of organosulfur compounds (OSCs such as diallyl sulfide, diallyl
disulfide, diallyl trisulfide) that are believed to ultimately confer the main pharmacological
effects [89].
Recommended doses range around 4 g of fresh garlic daily, which is equivalent to approximately
8 mg garlic oil or 600 to 900 mg garlic powder preparations standardized to 1.3 %
alliin content [89].
Despite the widespread use of garlic preparations along with reported preclinical
evidence and anecdotal clinical case reports on the potential role of garlic in metabolism-based
PK interactions and also some susceptibility for PD interactions, well-designed clinical
studies evaluating drug interactions with garlic preparations in a systematic fashion
are notably very limited.
Nonclinical studies suggest that garlic components may exert enzyme inducing effects
on CYP1A1/2 and CYP2B1/2 family members via CAR and Nrf2 activation [90], and also to strongly induce GST activity [91], whereby upregulation of GST has been implicated in the detoxification of carcinogens.
In turn, inhibition of CYP2E1, CYP2C9, and 2C19 by garlic and QSCs was suggested by
preclinical data [69].
Unfortunately, the clinical relevance of these findings for commonly employed human
doses has not been completely followed up as yet by well-designed mechanistic in vivo studies for many of the described metabolic pathways. Hence, based on currently published
evidence, the question of clinical relevance for the described effects on CYP2B1/2
family members and UGTs, GST, QR, and EH enzymes remains virtually still unaddressed
to the best of our knowledge.
A total of 8 studies in healthy adult young and elderly subjects using different garlic
products (600 mg garlic tablets, 3 tablets b. i. d. [92]; garlic oil 500 mg, t. i. d. [18], [19], garlic oil extract, diallyl sulphide (DAS) [93]) and study designs have so far addressed various aspects of the PK drug interaction
potential of garlic treatment. Apparently, the most recent human in vivo study addressing garlic-related PK drug interaction issues was published in 2005,
thereby indicating some stagnation rather than ramp-up of clinical activities in this
area of research.
A total of 3 interaction studies with administration of prototypic enzyme-specific
probe drugs assessed the potential of various garlic preparations to alter activities
of CYP2E1 [93], CYP2D6 and 3A4 [92], CYP1A2, CYP2D6, CYP2E1, or CYP3A4 [17] in healthy subjects, and one study used
a similar approach to probe for alterations of CYP1A2, CYP2D6, CYP2E1, and CYP3A4
activities in elderly individuals [18].
These studies indicate consistently that neither single nor repeat dose administrations
of various garlic formulations up to 28 days may have potential to exert notable alterations
(i.e., inhibition or induction) of CYP1A2, CYP2D6, or CYP3A4 activities in humans,
which are important metabolic pathways for many drugs likely to be coadministered
with garlic supplements. In clinical terms, these results indicate that garlic preparations
are unlikely to alter the disposition of coadministered drugs that are sensitive substrates
of these oxidative metabolic pathways.
In contrast, repeat administration of garlic oil over 28 days was shown to modestly
reduce CYP2E1 activity [estimated by 6- hydroxychlorzoxazone (6-OH-CZX)/chlorzoxazone
(CZX) metabolic ratio] by 39 % in healthy adult subjects [17] and by approximately 22 % in elderly individuals (mean age 67 years) [18]. These results are consistent with an earlier study on single dose effects of 0.2 mg/kg
BW garlic oil extract, diallyl sulphide (DAS), on CYP2E1 activity, which also employed
the 6-OH-CZX/CZX metabolic ratio for the estimation of CYP2E1 function [93]. However, as the overall extent of CYP2E1 inhibition by garlic is modest, i.e.,
conceptually metabolic ratios always display quantitatively greater changes than respective
clearance alterations of the prototypic substrate, and hardly any currently approved
drugs in widespread use are relying on CYP2E1 as a sole or predominant clearance pathway
(except chlorzoxazone), the observed CYP2E1 effects of garlic do not appear to have
important clinical implications. This perspective is further supported by a study
in healthy adult subjects, in which a 3-month administration of aged garlic extract
(10 mL q. d.) did not show any notable effect on oxidative metabolism of acetaminophen,
to which CYP2E1 is known to be a significant contributor, besides CYP3A4 and CYP1A2
[94]. Interestingly, in this study also no effects of garlic on GST activity could
be noted, as no changes in the excretion of three acetaminophen metabolites arising
from GST-mediated glutathione conjugation with the reactive acetaminophen metabolite
were observed [94]. This finding casts doubts on the human in vivo relevance of the consistently reported inducing effects of garlic components on GST
activity from animal and in vitro studies.
The lack of a 3-week garlic treatment (GarliPure™ 1.200 mg/day) on the functional
activity of intestinal and hepatic CYP3A4 in healthy adult subjects was further confirmed
by demonstration of an unaltered PK of the HIV-protease inhibitor saquinavir, a sensitive
CYP3A4 substrate which undergoes extensive CYP3A4-mediated presystemic gut wall metabolism,
and by means of an erythromycin breath test, a noninvasive marker of hepatic CYP3A4
function [95].
These findings are also in line with another healthy subject study demonstrating that
a 4-day short-term treatment with garlic capsules (10 mg garlic extract q. d., provided
as 2 × 5-mg liquid-filled soft gelatin capsules, equivalent to 1 g of fresh garlic)
did not significantly alter the single dose PK of ritonavir, another HIV-protease
inhibitor that is mainly metabolized by CYP3A4. However, the authors cautioned that
in view of the complex time-dependent effects of ritonavir on drug metabolizing enzymes
and transporters (ritonavir is also a potent inhibitor of CYP3A4 and the ABC efflux
transporter P-gp), the results of the study may be difficult to interpret and should
not be extrapolated to steady-state conditions [96].
The only study reporting a PK alteration of the HIV-protease inhibitor saquinavir
upon a 3-week garlic treatment (GarliPure™ caplets b. i. d.) of ten healthy subjects
suggested about 51 %, 49 %, and 54 % reduced mean saquinavir steady-state exposure
(AUCτ), trough levels (Ctrough), and maximum concentrations (Cmax), respectively [55]. However, most notably, after a 10-day washout period of garlic treatment, the saquinavir
AUC, trough, and Cmax values did not return to baseline, but still remained at 60–70 % of their baseline
values. As the study employed a fixed sequence rather than a randomized crossover
design, and in light of published evidence on time-dependent saquinavir PK characteristics
in healthy subjects [97] and HIV patients [98], it is most conceivable that the study results merely mirror saquinavir-mediated
induction processes of its own metabolism and disposition rather than indicating a
relevant garlic-mediated drug interaction.
Taken together, there is well-consistent evidence from a variety of mechanistic PK
studies that common doses of various kinds of garlic preparations do not exert any
notable alteration of CYP3A4 function in vivo.
Whether or not garlic may be involved in transporter-based drug interactions has apparently
not yet been addressed in mechanistic human PK studies, to the best of our knowledge.
Also the preclinical evidence in this respect is less well documented [69].
Taken together, from the limited available evidence of human in vivo drug interaction studies, there is currently no indication that garlic may confer
any notable or clinical significant metabolism-based PK interactions with drugs cleared
by CYP1A2, CYP2D6, CYP2E1, or CYP3A4 enzymes. In contrast, unfortunately, no published
evidence from human in vivo studies is currently available for the characterization of the potential effects
of garlic on CYP2B family members and UGT, GST, QR, and EH enzymes or drug transporter
proteins. Hence, translation of published in vitro findings into clinical interaction studies is still largely incomplete.
Echinacea
Echinacea is a genus of herbaceous flowering plants (purple coneflowers, family Asteraceae),
overall comprising nine species, which are native and endemic to eastern and central
North America. Marketed and studied medicinal products are made of three different
species (E. purpurea, E. angustifolia, E. pallida), whereby the majority (i.e., about 80 %) of the products are based on E. purpurea. Different parts of the plants (aerial parts and roots) are used, and different pharmaceutical
preparations exist (extracts and expressed juice). The German Monograph issued by
the E Commission recommends alcoholic extracts from the root of E. pallida and juices pressed from the aerial parts of the plants [99]. However, the diversity in the overall composition and contents of pharmaceutical
Echinacea products is considerable and may contribute to some across study inconsistencies.
Echinacea is considered to be an unspecific immunostimulant and is primarily used for the prevention
and treatment of acute virus infections of the upper respiratory tract, such as the
common cold and influenza. Dose recommendations for Echinacea products vary widely and depend on the formulation and concentration of the product.
Recommended daily doses for adult subjects range between 900 to 1000 mg, to be given
in 3 to 4 divided doses.
Despite the popularity and widespread use of Echinacea preparations and their pharmaceutical diversity, well-designed clinical studies evaluating
PK drug interactions with different Echinacea preparations are notably very limited. Overall, we identified only 7 clinical PK
interaction studies of Echinacea products in the public domain, which primarily addressed CYP- and P-gp-based interaction
issues.
The effect of Echinacea (E. purpurea root) on CYP activities in vivo was assessed by use of single dose administrations of the CYP probe drugs caffeine
(CYP1A2), tolbutamide (CYP2C9), dextromethorphan (CYP2D6), and midazolam (CYP3A) in
12 healthy subjects (6 m/6 f) [100]. The probe substances caffeine (200 mg), tolbutamide (500 mg), dextromethorphan
(30 mg), and oral and intravenous midazolam (MDZ; 0.05 mg/kg BW i. v., and 5 mg per
oral) were administered before and after a short course of Echinacea [E. purpurea root extract 400 mg 4 times daily (= 1600 mg/day) for 8 days], and respective plasma
concentration-time profiles of the probe substances were assessed.
The disposition (i.e., AUC0-∞, oral clearance, t1/2) of the CYP2D6 probe dextromethorphan in CYP2D6 genotyped subjects (11 CYP2D6 EMs,
1 CYP2D6 PM) was not significantly altered by Echinacea. Likewise, the ratio of the dextromethorphan/dextrorphan AUC0-∞ values and the dextromethorphan-to-dextrorphan urinary metabolic ratio were not significantly
affected by Echinacea coadministration.
The oral clearance of the CYP2C9 probe drug tolbutamide was significantly, albeit
modestly, reduced on average by 11 % after Echinacea dosing, with two individuals (1 m/1 f) displaying a 25 % or greater reduction in
the oral clearance of tolbutamide. In line with this, the geometric mean total tolbutamide
exposure (AUC0-∞) before and after Echinacea dosing was modestly increased by 14 % (90 % CI 9–20 %). The maximum tolbutamide concentration
was not significantly altered by Echinacea.
Echinacea dosing significantly reduced the mean oral clearance of the CYP1A2 probe caffeine
by 27 %, with considerable intersubject variability (> 50 % in 2 individuals). Maximum
serum concentrations of caffeine after a 200-mg oral dose were on average increased
by 30 %, and the geometric mean total caffeine exposure (AUC0-∞) was increased by 29 % after Echinacea dosing.
The systemic clearance of intravenously administered CYP3A4 probe MDZ was significantly
increased by 42 %, and there was a corresponding significant decrease in mean AUC0-∞ of 23 %, together suggesting that hepatic CYP3A activity was increased. In contrast,
the oral clearance and the AUC0-∞ after oral MDZ dosing were not significantly altered by Echinacea administration. However, the mean absolute oral bioavailability (F) of MDZ was increased
from 24 % to 36 % (i.e., by 50 %), consistent with the inhibition of intestinal CYP3A
by Echinacea.
The authors discussed that the differential effects of Echinacea on intestinal and hepatic CYP3A activity may be due to a variety of possible mechanisms
such as locally acting CYP3A inhibiting constituents of Echinacea that do not become systemically available, or rapid absorption of CYP3A4 inducing
components of Echinacea, thereby limiting intestinal exposure and intestinal CYP3A induction, or a systemically
formed metabolite of a constituent of Echinacea capable of inducing hepatic CYP3A but not intestinal CYP3A. However, these mechanistic
considerations remain speculative and deserve further investigation.
Taken together, the study showed that an 8-day treatment with relatively high doses
of E. purpurea root extract (i.e., 400 mg 4 times daily = 1600 mg/day) displayed differential effects
on the activity of various CYP enzymes. There were no alterations of CYP2D6 noted,
negligible to modest inhibition of CYP2C9, modest inhibition of CYP1A2, and differential
(i.e., inductive/inhibitory) effects on hepatic and intestinal CYP3A4.
Although the observed mean effects on CYP1A2 and CYP2C9 activities were generally
modest, they displayed some more pronounced effect sizes in individual subjects. Considering
CYP-based Echinacea-drug interactions of potential clinical significance, the study suggests that the
bioavailability of orally administered CYP3A4 substrates with low oral bioavailability
may be significantly increased by Echinacea coadministration, and that the exposure to CYP1A2 and 2C9 substrates (e.g., theophylline,
clozapin) may be modestly increased, at least in individual subjects. The observed
effects of high doses of E. purpurea root extract on CYP1A2 and CYP2C9, however, are overall small and therefore unlikely
to be of clinical significance in clinical practice, unless substrates with a narrow
therapeutic index are concerned [e.g., (S)-warfarin in case of CYP2C9 and theophylline
in case of CYP1A2].
In another study, 12 healthy subjects (6 m/6 f) were randomly assigned to receive
E. purpurea (800 mg b. i. d., no standardization claim) for 28 days [50]. Probe drug cocktails of MDZ (CYP3A4) and caffeine (CYP1A2), followed 24 hours later
by chlorzoxazone (CYP2E1) and debrisoquine (CYP2D6) were administered before (baseline)
and at the end of supplementation. Presupplementation and postsupplementation assessments
of CYP3A4, CYP1A2, CYP2E1, and CYP2D6 activities were made by use of 1-OH-MDZ/MDZ
serum ratios (1-hour sample), paraxanthine/caffeine serum ratios (6-hour sample),
6-hydroxychlorzoxazone/chlorzoxazone serum ratios (2-hour sample), and debrisoquine
urinary recovery ratios (8-hour collection), respectively.
In this study, high-dose E. purpurea given over 28 days did not significantly change the activities of CYP3A4, CYP2E1,
and CYP2D6 as estimated by single timepoint metabolic ratios. The only perceptible
change in mean phenotypic ratios occurred with 6-hour paraxanthine/caffeine values,
where an approximate 13 % decrease (from 0.514 ± 0.181 to 0.447 ± 0.190) pointed to
at a possible inhibitory effect of E. purpurea on CYP1A2. This minor difference, however, was not statistically significant (p = 0.07),
nor was it considered clinically relevant.
The effects of supplementation of a standardized E. purpurea extract (267 mg t. i. d. = 801 mg/day, each capsule standardized to contain 2.2 mg
isobutylamides; Gaia Herbs, Inc.) on the activity of CYP2D6 was investigated in 16
healthy subjects (8 m/8 f) after administration of the CYP2D6 substrate debrisoquine
(5 mg) before and at the end of supplementation, by using 8-h debrisoquine urinary
recovery ratios [53]. Respective results did not indicate significant inhibition of CYP2D6 by E. purpurea supplementation.
Two studies examined the effects of Echinacea treatment of the PK of two different protease inhibitor/ritonavir combination products.
The most recent study was conducted in 15 HIV-infected patients (14 m/1 f, median
age 49 yrs, range 43 to 67 yrs) receiving antiretroviral therapy including darunavir-ritonavir
(600/100 mg b. i. d.) for at least 4 weeks. The patients were concomitantly treated
with E. purpurea root extract capsules (500 mg every 6 h) for 14 days on top of their antiretroviral
treatment [101]. The HIV protease inhibitor darunavir undergoes extensive metabolism by CYP3A4,
while ritonavir is a potent inhibitor of CYP3A4 and P-gp, and is combined with darunavir
to boost (i.e., increase) its systemic exposure. Darunavir and ritonavir plasma concentrations
were determined up to 12 h after a morning dose of darunavir-ritonavir on days 0 (darunavir-ritonavir
alone) and 14 (darunavir-ritonavir plus Echinacea). Mean ritonavir PK parameters were essentially unchanged by coadministration of
E. purpurea, while small changes in average darunavir PK parameters were noted, with trough concentrations
(Cτ) and measures of total exposure over a complete dosing interval (AUCτ) decreasing
on average by 16 % and 10 %, respectively. However, individual patients displayed
declines in darunavir Cτ or AUCτ by as much as 40 % and 30 %, respectively, while
maximum observed darunavir concentrations (Cmax) were essentially unchanged by E. purpurea coadministration. Overall, the study indicates that high-dose E. purpurea coadministration does not alter the PK of low dose ritonavir and has on average little
effect on the exposure characteristics of darunavir. It is to be noted, however, that
studies with ritonavir containing anti-HIV combination products provide little assay
sensitivity to capture any Echinacea-mediated effects on CYP3A4 activity because ritonavir, as a potent CYP3A4 inhibitor,
is likely to mask any less potent influence of Echinacea on CYP3A4 activity. However, the finding that darunavir exposure was reduced in individual
patients by 30 to 40 % upon E. purpurea coadministration, would be consistent with the assumption of a modest induction of
hepatic CYP3A4 by Echinacea administration as previously reported by Gorski and colleagues [50]. This finding would be further compatible with the hypothesis, that low-dose ritonavir
(100 mg b. i. d.) may not effectively inhibit hepatic CYP3A4 activity in individual
patients.
In a comparable study in thirteen healthy subjects (8 m/5 f) receiving a combination
of lopinavir/ritonavir 400/100 mg b. i. d. for 29.5 days, subjects were treated concomitantly
with E. purpurea (500 mg t. i. d.) for 28 days: 14 days in combination with lopinavir/ritonavir and
14 days of E. purpurea alone [102]. Lopinavir and ritonavir plasma concentrations were determined at the end of each
treatment period up to 12 h postdose. In order to assess CYP3A and P-gp activity,
subjects also received single oral doses of the probe drugs MDZ 8 mg (CYP3A4) and
fexofenadine 120 mg (P-gp/OATP2B1), respectively, before and after the 28 days of
E. purpurea. In this study, neither lopinavir nor ritonavir PK were significantly altered by
14 days of E. purpurea coadministration. The post-Echinacea:pre-Echinacea geometric mean ratios (GMRs) for the lopinavir AUC0–12 h and for maximum concentrations (Cmax) were 0.96 (90 % CI 0.83–1.10, p = 0.82) and 1.00 (90 % CI 0.88–1.12, p = 0.72),
respectively. Conversely, GMRs for the total exposure of MDZ (AUC0-∞) and oral MDZ clearance were 0.73 (90 % CI 0.61–0.85, p = 0.008) and 1.37 (90 % CI
1.10–1.63, p = 0.02), respectively, thereby suggesting the induction of CYP3A4-mediated
MDZ clearance. Fexofenadine PK, in contrast, did not significantly differ before and
after E. purpurea administration (p > 0.05).
Overall, the study outcome regarding the observed changes in CYP3A4 activity is consistent
with the results reported by Moltó for the effects of E. purpurea supplementation on a darunavir-ritonavir combination [101]. Both studies consistently indicate that E. purpurea coadministration is unlikely to significantly alter the PK of ritonavir-boosted protease
inhibitors, most likely due to the presence of the potent CYP3A inhibitor ritonavir,
but still may be capable to modestly induce hepatic CYP3A activity. The lack of any
effects of E. purpurea treatment on the PK of fexofenadine – a substrate of several drug transporters including
P-gp, OATP1A2, OATP1B1, and OATP2B1 – in the presence of low-dose ritonavir administration
is difficult to interpret, because ritonavir is also a potent inhibitor of P-gp and
hence, may mask any modest Echinacea-mediated effects on P-gp (ABCB1).
Abdul et al. investigated the PK and PD interactions of Echinacea with warfarin in 12 healthy male subjects who received a single oral 25 mg dose of
warfarin alone and after 2 weeks of pretreatment with high-dose Echinacea (1275 mg four times daily = 5100 mg/day, containing a mixture of 600 mg of E. angustifolia roots and 675 mg of E. purpurea root; standardized to contain 5.75 mg of total alkylamides per tablet) [103]. In this study, the apparent clearance of (S)-warfarin was found slightly increased
by 9 % (geom. mean ratio 1.09; 90 % CI of ratio 1.01, 1.18) during concomitant Echinacea treatment, a negligible change that achieved, however, statistical significance.
Accordingly, the total exposure (i.e., AUC0-∞) to (S)-warfarin was increased by 9 % [geom. mean ratio 1.09 (1.01, 1.18)]. The exposure
characteristics of (R)-warfarin were not changed significantly by Echinacea. Similarly, the PD endpoints were not altered significantly by Echinacea with geometric mean ratios of 1.04 (0.95, 1.13) and 1.09 (0.91, 1.31) for INRmax and AUCINR, respectively. As (S)-warfarin is metabolized by CYP2C9 and CYP3A4, and (R)-warfarin
is metabolized by CYP3A4 and CYP1A2 [104], it can be concluded that a 2-week treatment with high doses of Echinacea (i.e., 5100 mg/day) does not substantially alter the activities of these CYP enzymes.
Overall, the small effect sizes observed are unlikely to be of clinical relevance.
The effects of Echinacea supplementation on P-gp function was studied in 18 healthy subjects, randomly assigned
to receive a standardized Echinacea supplement (267 mg t. i. d. = 801 mg/day) for 14 days [105]. Subjects were also randomized to receive rifampin (300 mg b. i. d., 7 days) and
clarithromycin (500 mg b. i. d., 7 days) as positive controls for P-gp induction and
inhibition, respectively. Digoxin (Lanoxin™ 0.25 mg), a well-acknowledged P-gp probe,
was administered orally before and after each supplementation and control period.
Serial digoxin plasma concentrations were obtained over 24 h. Comparisons of area
under the curve values (AUC0–3, AUC0–24), terminal disposition half-life (t1/2), and maximum serum concentrations (Cmax) were used to assess the effects of Echinacea, rifampin, and clarithromycin on digoxin disposition. As expected, rifampin produced
significant reductions (p < 0.05) in AUC0–3, AUC0–24, and Cmax, while clarithromycin, a known P-gp (ABCB1) inhibitor, increased these parameters
significantly (p < 0.05). Echinacea supplementation, in contrast, did not affect digoxin PK, indicating that clinically
significant P-gp-mediated herb-drug interactions with Echinacea are unlikely to occur.
In conclusion, the available clinical evidence indicates that Echinacea products at recommended doses do have little potential to cause clinically relevant
or worrisome metabolism- and transporter-based PK interactions that involve CYP1A2,
CYP2C9, CYP2D6, CYP2E1, and P-gp. In turn, there is consistent clinical evidence suggesting
that Echinacea products actually do have potential to modestly induce hepatic CYP3A4 activity, but
at the same time also may inhibit the presystemic metabolism of CYP3A4 substrates
in the intestine. As both mechanisms in principle counteract each other in terms of
the net effects on the systemic exposure of CYP3A4 substrates, may require different
times for their onset (i.e., onset of inhibition occurs faster than onset of induction),
and also will depend in their effect size on specific substrate characteristics (e.g.,
oral bioavailability of CYP3A4 substrates), general predictions on the clinical relevance
of Echinacea/CYP3A4 substrate interactions are difficult to make. However, caution is advised
when CYP3A4 substrates with low oral bioavailability due to pronounced intestinal
CYP3A4-mediated metabolism such as verapamil, cyclosporine, or tacrolimus or narrow
therapeutic index drugs predominantly cleared by CYP3A4 are coadministered with Echinacea supplementation.
Evidence from two phenotyping studies employing daily Echinacea doses of 1600 mg [50], [100] suggests the potential of a weak inhibitory effect of high-dose Echinacea supplementation on CYP1A2 and 2C9. However, reported effect sizes are overall small
and therefore unlikely to be of clinical significance in clinical practice, unless
substrates with a narrow therapeutic index are concerned, such as warfarin (CYP2C9)
or theophylline (CYP1A2).
No clinical studies are available addressing the potential impact of Echinacea products on other important metabolic pathways such as CYP2C19 or phase II metabolism
(e.g., glucuronidation), and no clinical studies on potential transporter interactions
other than P-gp (ABCB1) are available as yet.
