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Planta Med 2013; 79(08): 616-621
DOI: 10.1055/s-0032-1328364
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Effect of Topical Application of Chlorogenic Acid on Excision Wound Healing in Rats

Wei-Cheng Chen
1   Department of Bioengineering, Tatung University, Taipei City, Taiwan
,
Shorong-Shii Liou
2   Department of Pharmacy & Graduate Institute of Pharmaceutical Technology, Tajen University, Yanpu Shiang, Ping Tung Shien, Taiwan
,
Thing-Fong Tzeng
3   Department of Internal Medicine, Pao Chien Hospital, Ping Tung City, Pingtung County, Taiwan
,
Shiow-Ling Lee
1   Department of Bioengineering, Tatung University, Taipei City, Taiwan
,
I-Min Liu
2   Department of Pharmacy & Graduate Institute of Pharmaceutical Technology, Tajen University, Yanpu Shiang, Ping Tung Shien, Taiwan
› Author Affiliations
Further Information

Publication History

received 06 December 2012
revised 23 February 2013

accepted 27 February 2013

Publication Date:
08 April 2013 (online)

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Abstract

This study was undertaken to evaluate the therapeutic effects of topical chlorogenic acid on excision wounds in Wistar rats. A 1 % (w/w) chlorogenic acid or silver sulfadiazine ointment was applied topically once a day for 15 days on full-thickness excision wounds created on rats. The 1 % (w/w) chlorogenic acid ointment had potent wound healing capacity as evident from the wound contraction on the 15th post-surgery day, which was similar to that produced by 1 % (w/w) silver sulfadiazine ointment. Increased rates of epithelialization were observed in the treated rats. It also improved cellular proliferation, increased tumor necrosis factor-α levels during the inflammatory phase (12 h, 24 h, 48 h, and 72 h post-wounding) of wound healing, upregulated transforming growth factor-β1 and elevated collagen IV synthesis in the chlorogenic acid-treated group. The results also indicated that chlorogenic acid possesses potent antioxidant activity by increasing superoxide dismutase, catalase, and glutathione, and decreasing lipid peroxidation. In conclusion, these results demonstrate that topical application of chlorogenic acid can accelerate the process of excision wound healing by its ability to increase collagen synthesis through upregulation of key players such as tumor necrosis factor-α and transforming growth factor-β1 in different phases of wound healing as well as by its antioxidant potential.

Key words

chlorogenic acid - collagen - TGF-β1 - TNF-α - wound healing
 

  • References

  • 1 Gantwerker EA, Hom DB. Skin: histology and physiology of wound healing. Facial Plast Surg Clin North Am 2011; 19: 441-453
    CrossrefPubMedGoogle Scholar
  • 2 Clark RA. Biology of dermal wound repair. Dermatol Clin 1993; 11: 647-666
    PubMedGoogle Scholar
  • 3 Lamme EN, Van Leeuwen RTJ, Brandsma K, Van Marle J, Middelkoop E. Higher numbers of autologous fibroblasts in an artificial dermal substitute improve tissue regeneration and modulate scar tissue formation. J Pathol 2000; 190: 595-603
    CrossrefPubMedGoogle Scholar
  • 4 Gupta A, Singh RL, Raghubir R. Antioxidant status during cutaneous wound healing in immunocompromised rats. Mol Cell Biochem 2002; 241: 1-7
    CrossrefPubMedGoogle Scholar
  • 5 Russo A, Longo R, Vanella A. Antioxidant activity of propolis: role of caffeic acid phenethyl ester and galangin. Fitoterapia 2002; 73: 21-29
    CrossrefPubMedGoogle Scholar
  • 6 James TJ, Hughes MA, Hofman D, Cherry GW, Taylor RP. Antioxidant characteristics chronic wound fluid. Br J Dermatol 2001; 145: 185-186
    PubMedGoogle Scholar
  • 7 Clifford MN. Diet-derived phenols in plasma and tissues and their implications for health. Planta Med 2004; 70: 1103-1114
    Article in Thieme ConnectPubMedGoogle Scholar
  • 8 Sies H, Stahl W, Sevanian A. Nutritional, dietary and postprandial oxidative stress. J Nutr 2005; 135: 969-972
    PubMedGoogle Scholar
  • 9 Wang Y, Ho CT. Polyphenolic chemistry of tea and coffee: a century of progress. J Agric Food Chem 2009; 57: 8109-8114
    CrossrefPubMedGoogle Scholar
  • 10 Huang X. Current status and prospect of the study of Lonicera japonica Thunb. Zhongguo Zhong Yao Za Zhi 1997; 22: 247-249
    PubMedGoogle Scholar
  • 11 Xu JG, Hu QP, Liu Y. Antioxidant and DNA-protective activities of chlorogenic acid isomers. J Agric Food Chem 2012; 60: 11625-11630
    CrossrefPubMedGoogle Scholar
  • 12 Lee AR, Moon HK. Effect of topically applied silver sulphadiazine on fibroblast cell proliferation and biochemical properties of wound. Arch Pharm Res 2003; 26: 855-860
    CrossrefPubMedGoogle Scholar
  • 13 Romero-Cerecero O, Zamilpa-Álvarez A, Ramos-Mora A, Alonso-Cortés D, Jiménez-Ferrer JE, Huerta-Reyes ME, Tortoriello J. Effect on the wound healing process and in vitro cell proliferation by the medicinal Mexican plant Ageratina pichinchensis . Planta Med 2011; 77: 979-983
    Article in Thieme ConnectPubMedGoogle Scholar
  • 14 Niehaus WG, Samuelsson B. Formation of malondialdehyde from phospholipid arachidonate during microsomal lipid peroxidation. Eur J Biochem 1968; 6: 126-130
    CrossrefPubMedGoogle Scholar
  • 15 Ellman GL. Tissue sulphydryl groups. Arch Biochem Biophys 1959; 82: 70-77
    CrossrefPubMedGoogle Scholar
  • 16 Kuthan H, Haussmann HJ, Werringloer J. A spectrophotometric assay for superoxide dismutase activities in crude tissue fractions. Biochem J 1986; 237: 175-180
    PubMedGoogle Scholar
  • 17 Sinha KA. Colorimetric assay of catalase. Anal Biochem 1972; 47: 389-394
    CrossrefPubMedGoogle Scholar
  • 18 Adams PD. Healing and hurting: molecular mechanisms, functions, and pathologies of cellular senescence. Mol Cell 2009; 36: 2-14
    CrossrefPubMedGoogle Scholar
  • 19 Wong T, McGrath JA, Navsaria H. The role of fibroblasts in tissue engineering and regeneration. Br J Dermatol 2007; 156: 1149-1155
    CrossrefPubMedGoogle Scholar
  • 20 Schultz GS, Wysocki A. Interactions between extracellular matrix and growth factors in wound healing. Wound Repair Regen 2009; 17: 153-162
    CrossrefPubMedGoogle Scholar
  • 21 Abreu-Velez AM, Howard MS. Collagen IV in normal skin and in pathological processes. N Am J Med Sci 2012; 4: 1-8
    CrossrefPubMedGoogle Scholar
  • 22 Lim Y, Levya MA, Bray TM. Dietary supplementation of N-acetylcysteine enhances early inflammatory responses during cutaneous wound healing in protein malnourished mice B. J Nutr Biochem 2006; 17: 328-336
    CrossrefPubMedGoogle Scholar
  • 23 Lawrence WT, Diegelmann RF. Growth factors in wound healing. Clin Dermatol 1994; 12: 157-169
    CrossrefPubMedGoogle Scholar
  • 24 Caiado F, Carvalho T, Silva F, Castro C, Clode N, Dye JF, Dias S. The role of fibrin E on the modulation of endothelial progenitors adhesion, differentiation and angiogenic growth factor production and the promotion of wound healing. Biomaterials 2011; 32: 7096-7105
    CrossrefPubMedGoogle Scholar
  • 25 Atiba A, Nishimura M, Kakinuma S, Hiraoka T, Goryo M, Shimada Y, Ueno H, Uzuka Y. Aloe vera oral administration accelerates acute radiation-delayed wound healing by stimulating transforming growth factor-β and fibroblast growth factor production. Am J Surg 2011; 201: 809-818
    CrossrefPubMedGoogle Scholar
  • 26 Jiang F, Zhang Y, Dusting GJ. NADPH oxidase-mediated redox signaling: roles in cellular stress response, stress tolerance, and tissue repair. Pharmacol Rev 2011; 63: 218-242
    CrossrefPubMedGoogle Scholar
  • 27 Gouthamchandra K, Mahmood R, Manjunatha H. Free radical scavenging, antioxidant enzymes and wound healing activities of leaves extracts from Clerodendrum infortunatum L. Environ Toxicol Pharmacol 2010; 30: 11-18
    CrossrefPubMedGoogle Scholar