Lupinifolin extracted from Derris reticulata inhibits growth of Staphylococcus aureus possibly by damaging bacterial cell membrane

K Yusook; O Weeranantanapan; S Riyajan; J Musika; N Chudapongse

doi:10.1055/s-0036-1596513

Planta Medica, Inhaltsverzeichnis

Planta Med 2016; 82(S 01): S1-S381
DOI: 10.1055/s-0036-1596513

Abstracts

Georg Thieme Verlag KG Stuttgart · New York

Lupinifolin extracted from Derris reticulata inhibits growth of Staphylococcus aureus possibly by damaging bacterial cell membrane

Authors

K Yusook

¹School of Pharmacology, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand
O Weeranantanapan

²School of Anatomy, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand
S Riyajan

¹School of Pharmacology, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand
J Musika

¹School of Pharmacology, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand
N Chudapongse

¹School of Pharmacology, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand

Abstract

Volltext

Lupinifolin is a prenylated flavanone that has been isolated from several medicinal plants, such as Myriopteron extensum [1], Eriosema chinense [2] and Albizia myriophylla [3]. It is also reported to be a major compound of Derris reticulata [4]. In the present study, lupinifolin was isolated from Derris reticulata stem and identified by NMR spectra. Because of its nonpolar structure, lupinifolin is very soluble in organic solvents, but sparingly soluble in water. Estimated from log Kow (Octanol-Water Partition Coefficient), water solubility of lupinifolin at 25 °C is 0.009 mg/L [5]. When dissolved in alcohol or dimethyl sulfoxide, it precipitates after diluted in aqueous buffer. In this study, to avoid the precipitation in aqueous media, lupinifolin was freshly prepared by solubilizing in 0.5 N NaOH and immediately diluted in Müller-Hinton broth (MHB) for antibacterial test. From two-fold microdilution method, it was found that lupinifolin possessed antimicrobial activity against Staphylococcus aureus with MIC and MBC of 8 and 16 µg/mL, respectively. The antibacterial activity of lupinifolin was confirmed by TEM. After incubation with MIC overnight, lupinifolin significantly decreased the number of bacteria compared to control and ruptured bacterial cell membrane and cell wall. We hypothesize that lupinifolin may alter bacterial membrane structure and function similar to plant flavonoids previously reported [6]. Confirmation of the mechanism underlying this activity of lupinifolin is in progress.

Acknowledgements: This investigation was supported by SUT Research and Development Fund. We thank Dr. Paul J. Grote for verification of plant botanical classification.

Keywords: Derris reticulata, lupinifolin, Staphylococcus aureus, antimicrobial, cell membrane.

References:

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