Introduction:
The Endocannbinoidsystem (ECS) has been recently identified in inflammatory reactions.
CB1-receptors are mainly present in central nervous system. CB2-Receptors has been
identified on inflammatory peripheral immune cells (e.g. T cells, B cells and mast
cells).
Methods:
15 samples of patient undergoing endoscopic sinus surgery (CRSwNP and CRSsNP) were
evaluated. Western blot was applied to detect CB1 and CB2 receptors on level of protein.
To overrun the results, the real-time PCR was performed on subgroups by endotyping
of patients with multiplex Elisa.
Results:
15 samples have been examined in human nasal mucosa. Two groups were set – group 1:
chronic rhinosinusitis with nasal polyps n = 11, group 2: chronic rhinosinusitis without
nasal polyps n = 4. Both receptors were detected in control tissue. CB1 receptors
were more present in the group of chronic rhinosinusitis without polyps and CB2 receptors
in the group of chronic rhinosinusitis with polyps.
Conclusion:
We were able to demonstrate CB1 and CB2 receptors in the human nasal mucosa in an
in vitro experimental model by application of a quantitative analysis. With the detection
of cannabinoid receptors, another interesting aspect opens up to explore the pathophysiology
of CRS in the coming years.
