Surface proteome of plasma extracellular vesicles as biomarkers for pneumonia and acute exacerbation of COPD

A Jung; M Møller Jørgensen; R Bæk; K Griss; M Han; K auf dem Brinke; N Timmesfeld; W Bertrams; T Greulich; AR Koczulla; S Hippenstiel; N Suttorp; B Schmeck

doi:10.1055/s-0039-3403103

Pneumologie, Inhaltsverzeichnis

Pneumologie 2020; 74(S 01): 20
DOI: 10.1055/s-0039-3403103

Posterbegehung (PO02) – Sektion Zellbiologie

Neue Innovationen in der Zellbiologie

Georg Thieme Verlag KG Stuttgart · New York

Surface proteome of plasma extracellular vesicles as biomarkers for pneumonia and acute exacerbation of COPD

Authors

A Jung

¹Institute for Lung Research, Universities of Giessen and Marburg Lung Center, Philipps-University Marburg, Member of the German Center for Lung Research (Dzl)
M Møller Jørgensen

²Department of Clinical Immunology, Aalborg University Hospital
R Bæk

²Department of Clinical Immunology, Aalborg University Hospital
K Griss

³Institute for Lung Research, Universities of Giessen and Marburg Lung Center, Philipps-University Marburg, Member of the German Center for Lung Research (Dzl); Medizinische Klinik M. S. Infektiologie und Pneumologie, Charité – Universitätsmedizin Berlin
M Han

⁴Institute for Lung Research, Universities of Giessen and Marburg Lung Center, Philipps-University Marburg, Member of the German Center for Lung Research (Dzl); Medizinische Klinik M. S. Hämatologie und Onkologie, Charité – Universitätsmedizin Berlin
K auf dem Brinke

¹Institute for Lung Research, Universities of Giessen and Marburg Lung Center, Philipps-University Marburg, Member of the German Center for Lung Research (Dzl)
N Timmesfeld

⁵Abteilung für Medizinische Informatik, Biometrie und Epidemiologie, Ruhr-Universität Bochum
W Bertrams

¹Institute for Lung Research, Universities of Giessen and Marburg Lung Center, Philipps-University Marburg, Member of the German Center for Lung Research (Dzl)
T Greulich

⁶Department of Medicine, Pulmonary and Critical Care Medicine, University Medical Center Giessen and Marburg, Philipps-University, Member of the German Center for Lung Research (Dzl)
AR Koczulla

⁶Department of Medicine, Pulmonary and Critical Care Medicine, University Medical Center Giessen and Marburg, Philipps-University, Member of the German Center for Lung Research (Dzl)
S Hippenstiel

⁷Medizinische Klinik M. S. Infektiologie und Pneumologie, Charité – Universitätsmedizin Berlin
N Suttorp

⁷Medizinische Klinik M. S. Infektiologie und Pneumologie, Charité – Universitätsmedizin Berlin
B Schmeck

⁸Institute for Lung Research, Universities of Giessen and Marburg Lung Center, Philipps-University Marburg, Member of the German Center for Lung Research (Dzl); Department of Medicine, Pulmonary and Critical Care Medicine, University Medical Center Giessen and Marburg, Philipps-University, Member of the German Center for Lung Research (Dzl)

Abstract

Volltext

Community-acquired pneumonia (CAP) and acute exacerbations of chronic obstructive pulmonary disease (AECOPD) represent a major burden of morbidity and mortality and their differential diagnosis is critical. A potential source of relevant accessible biomarkers are blood-borne small extracellular vesicles (sEVs).

We performed an EV Array to find proteins on plasma sEVs that are differentially expressed and possibly allow the differential diagnosis between CAP and AECOPD. Plasma samples from 21 healthy subjects, 24 CAP and 10 AECOPD patients were analyzed. The EV Array contained 40 antibodies to capture sEVs, which were then visualized with a cocktail of biotin-conjugated CD9, CD63 and CD81 antibodies.

We detected significant differences in the protein decoration of sEVs between healthy subjects and CAP or AECOPD patients. We found CD45 and CD28 to be the best discrimination markers between CAP and AECOPD in ROC analyses with an area under the curve of > 0.92. Additional ensemble feature selection revealed the possibility to distinguish between CAP and AECOPD even if the CAP patient had COPD, with a panel of CD45, CD28, CTLA4, TNF-R-II.

The discrimination of sEV-associated proteins is a minimally-invasive method with potential to discriminate between CAP and AECOPD patients.