Einleitung The most successful strategy to treat obesity and associated comorbidities is bariatric
surgery. This intervention is known to decrease body weight and to improve whole-body
insulin sensitivity, but the underlying molecular mechanisms remain unclear. The aim
of our study was to evaluate if bariatric surgery can correct the expression and DNA
methylation profiles in the skeletal muscle.
Methoden Genome-wide gene expression and DNA methylation were analyzed in skeletal muscle
biopsies of 6 lean controls CON and 16 obese humans OB (OB; 38 ± 10 ys, BMI 44 ± 10 kg/m2), at baseline (OB 0 w) and 52 weeks after bariatric surgery (OB 52 w).
Ergebnisse The comparison of the muscle transcriptome of OB 0 w and CON revealed 1,037 differentially
expressed genes which were enriched in lipid and reactive oxygen species pathways.
At 52 w, the expression of the majority genes (765) was normalized to levels of the
control group. Linking the DNA methylation changes induced by the bariatric surgery
to gene expression demonstrated that 349 of 765 identified genes were normalized to
the CON levels 52 w after the surgery.
Schlussfolgerung The transcriptome of skeletal muscle seems to benefit from the bariatric surgery
and some changes appear to be mediated by DNA methylation.
