During pomace oil production several by-products are produced in mills and specifically
wet olive pomace, dried olive pomace, exhausted olive cake, olive pomace skin and
olive stones. According to the type of by-product, they are characterized by the presence
of different classes of olive bioactive compounds, such as simple phenols and phenolic
acids, secoiridoids, flavonoids, lignans and triterpenic acids [1]. However, despite their high commercial interest there is only a limited number
of studies regarding their detail chemical composition and the targeted recovery of
bioactives. Thus, the initial aim of this work is the detailed chemical investigation
of these by-products using HPLC-DAD, LC-HRMS & HRMS/MS and HPTLC analytical methods.
Moreover, selective extraction and separation protocols were developed and applied
to produce chemical class-specific enriched extracts, employing Ultrasound Assisted
Extraction (UAE) and Fast Centrifugal Partition Chromatography (FCPC) techniques among
others. Overall, in the context of the present study, it was possible to produce extracts
enriched in secoiridoids or triterpenes or simple phenols from different types of
olive pomace by-products which could have potential applications in the food, cosmetic
and pharmaceutical industry.