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Planta Med 2017; 83(18): 1412-1419
DOI: 10.1055/s-0043-112344
Natural Product Chemistry and Analytical Studies
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Rapid Transient Production of a Monoclonal Antibody Neutralizing the Porcine Epidemic Diarrhea Virus (PEDV) in Nicotiana benthamiana and Lactuca sativa

Kaewta Rattanapisit
1   Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand
2   Herbs and Natural Products Research Unit, CU Drug and Health Products Innovation Promotion, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand
,
Anchalee Srijangwad
3   Veterinary Microbiology, Faculty of Veterinary Science, Bangkok, Thailand
,
Taksina Chuanasa
1   Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand
2   Herbs and Natural Products Research Unit, CU Drug and Health Products Innovation Promotion, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand
,
Suchada Sukrong
1   Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand
2   Herbs and Natural Products Research Unit, CU Drug and Health Products Innovation Promotion, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand
,
Angkana Tantituvanont
4   Pharmaceutics and Industrial Pharmacy, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand
,
Hugh S. Mason
5   The Biodesign Institute and School of Life Sciences, Arizona State University, Tempe, AZ, USA
,
Dachrit Nilubol
3   Veterinary Microbiology, Faculty of Veterinary Science, Bangkok, Thailand
,
Waranyoo Phoolcharoen
1   Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand
2   Herbs and Natural Products Research Unit, CU Drug and Health Products Innovation Promotion, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand
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Weitere Informationen

Publikationsverlauf

received 13. März 2017
revised 10. Mai 2017

accepted 22. Mai 2017

Publikationsdatum:
02. Juni 2017 (online)

    Lizenzen und Reprints

Abstract

Porcine epidemic diarrhea virus (PEDV) causes acute diarrhea, vomiting, dehydration, weight loss, and high mortality rate in neonatal piglets. Porcine epidemic diarrhea (PED) has been reported in Europe, America, and Asia including Thailand. The disease causes substantial losses to the swine industry in many countries. Presently, there is no effective PEDV vaccine available. In this study, we developed a plant-produced monoclonal antibody (mAb) 2C10 as a prophylactic candidate to prevent the PEDV infection. Recently, plant expression systems have gained interest as an alternative for the production of antibodies because of many advantages, such as low production cost, lack of human and animal pathogen, large scalability, etc. The 2C10 mAb was transiently expressed in Nicotiana benthamiana and lettuce using geminiviral vector. After purification by protein A affinity chromatography, the antibody was tested for the binding and neutralizing activity against PEDV. Our result showed that the plant produced 2C10 mAb can bind to the virus and also inhibit PEDV infection in vitro. These results show excellent potential for a plant-expressed 2C10 as a PEDV prophylaxis and a diagnostic for PEDV infection.

Key words

porcine epidemic diarrhea virus (PEDV) - 2C10 monoclonal antibody (mAb) - molecular farming - Nicotiana benthamiana - Solanaceae - Lactuca sativa - Asteraceae
 

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