Pancreatic β-cell function and notably glucose-induced insulin secretion play a pivotal
role in glucose homeostasis. We have previously shown that natural molecules like
flavonoids or ellagitannin metabolites potentiate glucose-induced insulin secretion
through a mechanism implicating the activation of L-type Ca2+(CaV) channels and ERK 1/2 [1]
[2]
[3]. According to some studies, extracts from Angelica L. species showed similar effects on insulin secretion [4]
[5]. Thus, this study aimed at isolating and studying active constituents of three pharmacopeial
Angelica roots.
Twenty-two coumarins were isolated from the roots of Angelica archangelica L., Angelica dahurica (Hoffm.) Benth. & Hook.f. ex Franch. & Sav., and Angelica pubescens Maxim through Soxhlet extraction in n-hexane, and column and preparative chromatography
methods. The structures of the compounds were analysed and confirmed using UHPLC-DAD-MS
and NMR methods. Pharmacological experiments were performed on the INS-1 β-cell line.
Insulin release was quantified by the homogeneous time- resolved fluorescence method.
The mechanism of action was studied using patch clamp and FACS techniques.
Some of the studied compounds were able to modulate glucose-induced insulin secretion
by acting on the membrane potential, as well as by activating voltage-gated calcium
channels and increasing intracellular calcium concentrations. Overall, the results
demonstrate a new pharmacological activity of coumarins present in traditionally used
Angelica roots.
Please note: article SL-YRW-07 was changed according to the
following erratum: The authors name Soufi yan Omhmmed was
misspelled, which is now corrected.
