Genetic association of five plasminogen activator inhibitor-1 (PAI-1) polymorphisms and idiopathic recurrent pregnancy loss in Korean women

Young Joo Jeon; Young Ran Kim; Bo Eun Lee; Yi Seul Choi; Ji Hyang Kim; Ji Eun Shin; HyungChul Rah; Sun Hee Cha; Woo Sik Lee; Nam Keun Kim

doi:10.1160/TH13-03-0242

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2013; 110(04): 742-750
DOI: 10.1160/TH13-03-0242

Blood Coagulation, Fibrinolysis and Cellular Haemostasis

Schattauer GmbH

Genetic association of five plasminogen activator inhibitor-1 (PAI-1) polymorphisms and idiopathic recurrent pregnancy loss in Korean women

Young Joo Jeon^*

¹Institute for Clinical Research, CHA Bundang Medical Center, School of Medicine, CHA University, Seongnam, South Korea

,

Young Ran Kim^*

²Department of Obstetrics and Gynecology, CHA Bundang Medical Center, School of Medicine, CHA University, Seongnam, South Korea

,

Bo Eun Lee

¹Institute for Clinical Research, CHA Bundang Medical Center, School of Medicine, CHA University, Seongnam, South Korea

,

Yi Seul Choi

¹Institute for Clinical Research, CHA Bundang Medical Center, School of Medicine, CHA University, Seongnam, South Korea

,

Ji Hyang Kim

²Department of Obstetrics and Gynecology, CHA Bundang Medical Center, School of Medicine, CHA University, Seongnam, South Korea

,

Ji Eun Shin

²Department of Obstetrics and Gynecology, CHA Bundang Medical Center, School of Medicine, CHA University, Seongnam, South Korea

,

HyungChul Rah

¹Institute for Clinical Research, CHA Bundang Medical Center, School of Medicine, CHA University, Seongnam, South Korea

,

Sun Hee Cha

²Department of Obstetrics and Gynecology, CHA Bundang Medical Center, School of Medicine, CHA University, Seongnam, South Korea

,

Woo Sik Lee

³Fertility Center of CHA Gangnam Medical Center, CHA University, Seoul, South Korea

,

Nam Keun Kim

¹Institute for Clinical Research, CHA Bundang Medical Center, School of Medicine, CHA University, Seongnam, South Korea

› Author Affiliations

Abstract

Summary

Plasminogen activator inhibitor-1 (PAI-1) is important for maintaining pregnancy. Aberrantly increased PAI-1 levels may contribute to thrombosis and inflammation, leading to pregnancy loss. This study investigated the association of PAI-1 polymorphisms (PAI-1 rs2227631 [-844G>A], rs1799889 [-675 4G/5G], rs6092 [43G>A], rs2227694 [9785G>A], and rs7242 [11053T>G]) with idiopathic recurrent pregnancy loss (RPL) in Korean women. We screened 308 RPL patients and 227 control participants for five PAI-1 polymorphisms. Genotyping of PAI-1 was performed by polymerase chain reaction-restriction fragment length polymorphism assay. PAI-1 4G4G and -844AA/ 4G4G/11053GG genotypes were associated with RPL. PAI-1 -844A/4G/43G/9785G/11053G haplotype was connected to hypofibrinolytic status (i.e. increased levels of plasma PAI-1, increased numbers of platelets, reduced prothrombin time, and reduced activated partial thromboplastin time). Moreover, PAI-1 11053TG+GG frequency was positively related to plasma homocysteine and urate levels, whereas -844AA frequency was associated with plasma folate concentrations according to ordinal logistic regression analysis. Based on these results, we propose that PAI-1 -844G>A, 4G/5G, and 11053T>G polymorphisms are markers of RPL.

Keywords

Plasminogen activator inhibitor-1 - polymorphism - recurrent pregnancy loss

Full Text

References

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