Efficacy of micronized flavonoid fraction in healing of clean and infected wounds

Abstract

Purified micronized flavonoid fraction, comprising 90% diosmin and 10% hesperidin, is basically used as a phelebotonic and vasculoprotector agent. It also has anti-inflammatory and anti-edematous actions. This study was designed to evaluate the efficacy of micronized flavonoid fraction in clean and infectious wounds. Sixty Guinea pigs were separated into 2 main groups and 6 subgroups. The first three groups had clean wounds and the other three groups had infected wounds contaminated with S. aureus. Then these main groups were separated into 3 subgroups. The first subgroups was treated with 60 mg/kg/day micronized flavonoid fraction per oral, the second ones was treated with topical 60 mg/kg/day micronized flavonoid fraction, and the thrid ones had no treatment. Infected wounds were obtained by contamining the wounds with S. aureus. There were no significant differences in wound healing between the groups that have clean wounds (p > 0.05). In the groups with infected wounds, orally and topically treated guina pigs had accelerated wound healing compared to the untreated control group (p < 0.05), confirmed with surface area measurements and histopathological evaluation. Healing showed no difference between the groups treated orally and topically. This study showed that oral or topical administration of micronized flavonoid fraction in the infected wounds is beneficial, while it has no significant effect in clean wounds.