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Planta Med 2019; 85(07): 599-607
DOI: 10.1055/a-0824-1282
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Inhibitory Effects of Pterocarpus santalinus Extract against IgE/Antigen-Sensitized Mast Cells and Atopic Dermatitis-Like Skin Lesions

Baknoon Ham   *
1   Graduate School of Energy and Environment, Korea University, Seoul, South Korea
,
Myungsuk Kim   *
2   Natural Products Research Center, Korea Institute of Science and Technology, Gangneung, South Korea
3   Smart Farm Solution Convergence Research Center, Korea Institute of Science and Technology, Gangneung, South Korea
,
Yang-Ju Son
2   Natural Products Research Center, Korea Institute of Science and Technology, Gangneung, South Korea
3   Smart Farm Solution Convergence Research Center, Korea Institute of Science and Technology, Gangneung, South Korea
,
Sungyul Chang
3   Smart Farm Solution Convergence Research Center, Korea Institute of Science and Technology, Gangneung, South Korea
,
Sang Hoon Jung
2   Natural Products Research Center, Korea Institute of Science and Technology, Gangneung, South Korea
,
Chu Won Nho
3   Smart Farm Solution Convergence Research Center, Korea Institute of Science and Technology, Gangneung, South Korea
,
Man Jae Kwon
1   Graduate School of Energy and Environment, Korea University, Seoul, South Korea
4   Department of Earth and Environmental Sciences, Korea University, Seoul, South Korea
› Author Affiliations
Further Information

Publication History

received 03 August 2018
revised 10 November 2018

accepted 10 December 2018

Publication Date:
04 January 2019 (online)

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Abstract

Pterocarpus santalinus has been traditionally used as a medicinal plant owing to its anti-inflammatory, anthelmintic, tonic, antihyperglycemic, and diaphoretic properties. We hypothesized that P. santalinus might have therapeutic potential in alleviating allergy and atopic dermatitis. Thus, we investigated the inhibitory effects of P. santalinus extract against allergic responses and 2,4-dinitrochlorobenzene-induced atopic dermatitis-like dorsal skin lesions using immunoglobulin E-sensitized rat basophilic leukemia-2H3 mast cells and NC/Nga mice. Degranulation and enzyme-linked immunosorbent assays were conducted to measure degranulation, proinflammatory cytokine levels, and prostaglandin E2 concentrations in immunoglobulin E/antigen-sensitized RBL-2H3 mast cells. The therapeutic efficacy of P. santalinus extract in 2,4-dinitrochlorobenzene-induced atopic dermatitis was evaluated through morphological, physiological, and immunological analysis. P. santalinus extract inhibited β-hexosaminidase and histamine release and reduced tumor necrosis factor-α, interleukin-4, and prostaglandin E2 secretion. Furthermore, P. santalinus extract suppressed atopic dermatitis-like skin lesions by regulating the serum levels of immunoglobulin E and immunoglobulin G2a, and messenger ribonucleic acid expression of T helper cell 1- and T helper cell 2-related mediators in the skin lesions. Histopathological analyses showed a decrease in epidermal thickness and intradermal inflammatory cell infiltration. These results suggested that P. santalinus extract might have beneficial effects in treating allergic and atopic dermatitis-like skin disorders.

Key words

Pterocarpus santalinus - Leguminosae - RBL-2H3 mast cell - atopic dermatitis - IgE - Th1/Th2-related mediators

* These two authors contributed equally to the present study.


Supporting Information

    The HPLC chromatogram of P. santalinus 50% EtOH reflux extracts (PSE) and the experimental scheme are available in the Supporting Information.

  • Supporting Information
 

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