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Planta Med 2020; 86(01): 32-44
DOI: 10.1055/a-1031-7328
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Protective Role of Casuarinin from Melaleuca leucadendra against Ethanol-Induced Gastric Ulcer in Rats

Eman Al-Sayed
1   Department of Pharmacognosy, Faculty of Pharmacy, Ain-Shams University, Cairo, Egypt
,
Haidy E. Michel
2   Department of Pharmacology and Toxicology, Faculty of Pharmacy, Ain-Shams University, Cairo, Egypt
,
Mohamed Abdelrazik Khattab
3   Department of Cytology and Histology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt
,
Mohamed El-Shazly
1   Department of Pharmacognosy, Faculty of Pharmacy, Ain-Shams University, Cairo, Egypt
4   Department of Pharmaceutical Biology, Faculty of Pharmacy and Biotechnology, German University in Cairo, Cairo, Egypt
,
Abdel Nasser Singab
1   Department of Pharmacognosy, Faculty of Pharmacy, Ain-Shams University, Cairo, Egypt
› Author Affiliations
Further Information

Publication History

received 20 July 2019
revised 10 October 2019

accepted 18 October 2019

Publication Date:
05 November 2019 (online)

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Abstract

Gastric ulcer is a major health problem. Current treatment options of gastric ulcer, including antagonists of histamine H2 receptor and inhibitors of the proton pump, do not cure gastric ulcers, but only provide temporary relief of symptoms and can be associated with severe side effects. The lack of effective and safe medications for this global health problem urges for the discovery of novel classes of compounds with potent activity and an acceptable safety profile. Ethanol-induced ulceration in rats was used to evaluate the gastroprotective activity of casuarinin, an ellagitannin isolated from Melaleuca leucadendra. Casuarinin (25, 50, and 100 mg/kg) reduced the ulcer area by 45, 78, and 99%, respectively, compared with the ulcer group. Casuarinin (100 mg/kg) increased mucin content by 1.8-fold and reduced acidity by 42%. At the same dose, it also increased the levels of reduced glutathione by 194%, catalase by 586%, and prostaglandin E2 to its normal level. In contrast, it attenuated the ethanol-increased levels of malondialdehyde by 56%, TNF-α by 58%, and caspase-3 by 87%. Histological findings demonstrated that casuarinin exhibited a protective effect against tissue alterations in response to the ethanol-induced ulcer. Casuarinin suppressed the immunoexpression of nuclear factor-kappa B, cyclooxygenase-2, and inducible nitric oxide synthase to their normal values. It also induced the expression of heat shock protein-70, reaching up to 4.9-fold in comparison with the ulcer group. The potent gastroprotective effect of casuarinin was thus attributed to its anti-inflammatory, antioxidant, and antiapoptotic effects. Our results suggest the potential application of casuarinin as an antiulcer agent from natural sources.

Key words

Melaleuca leucadendra - myrtaceae - caspase-3 - COX-2 - ellagitannins - casuarinin

Supporting Information

    1H NMR, 13C NMR and UV spectra of casuarinin are available as Supporting Information.

  • Supporting Information
 

  • References

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