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Horm Metab Res 1977; 9(2): 128-131
DOI: 10.1055/s-0028-1093560
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© Georg Thieme Verlag KG Stuttgart · New York

Phosphofructokinase (Isozymes) Activation by Theophylline or by 3',5' Cyclic AMP Administration

M. F. Meldolesi , V.  Macchia , S.  Varrone
  • Centro di Endocrinologia ed Oncologia Sperimentale del C.N.R., Istituto di Patologia Generale, II Facoltà di Medicina, Università di Napoli, Naples, Italy
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Publication History

Publication Date:
23 December 2008 (online)

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Abstract

Phosphofructokinase activity of rat thyroid, kidney and muscle can be enhanced by in vivo administration of theophylline or cyclic AMP. This enhancement occurs in the different tissues to a different extent depending on the tissue-specific isozymes. Thyroid and muscle phosphofructokinase which is mainly A-type, is strongly stimulated after administering theophylline in the drinking water over a 8 days period. The kidney enzyme which encompasses about 50% of each A and B type is activated to a lesser extent. Liver phosphofructokinase which is pure B-type shows very little response if any. Comparable results have been obtained with either the muscle or liver enzyme after two to four hours treatment with a single injection of 10 mg/rat of either cyclic AMP or theophylline. This short-term activation could be eliminated by treating the animals with Actinomycin D, 30 min prior to theophylline or cyclic AMP. The possible mechanisms leading to the enhancement of phosphofructokinase activity are discussed.

Key words

Phosphofructokinase Activation - Phosphofructokinase Isozymes - Cyclic AMP

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