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Horm Metab Res 1976; 8(1): 16-24
DOI: 10.1055/s-0028-1093686
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© Georg Thieme Verlag KG Stuttgart · New York

Binding of Somatomedins and Insulin to Plasma Membranes Prepared from Rat and Monkey Tissue

K.  Takano , K.  Hall , L.  Fryklund , H.  Sievertsson
  • Department of Endocrinology and Metabolism, Karolinska sjukhuset, and AB KABI, the Recip Polypeptide Laboratory, Stockholm, Sweden
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Publication History

Publication Date:
23 December 2008 (online)

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Abstract

Particulate membranes prepared from a variety of monkey and rat tissues were shown to have specific binding sites for somatomedin A. Binding to talc on the other hand showed no specificity, and the sensitivity was less. The membrane bound somatomedin A was displaced by somatomedin A in concentrations between 0.01 and 5 U/ml. Of the other hormones tested only insulin in high concentrations could interfere with the binding of labeled somatomedin A to tissue membranes. Membranes prepared from a number of rat and monkey tissues also contained binding sites for insulin. Somatomedin A could interfere with the binding of labeled insulin. No binding was observed for somatomedin B in the tissues studied.

Key words

Somatomedin - Insulin - Binding Sites

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