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Horm Metab Res 1974; 6(5): 357-364
DOI: 10.1055/s-0028-1093825
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© Georg Thieme Verlag KG Stuttgart · New York

The Role of Calcium in Insulin Action - II. Effects of Insulin and Procaine Hydrochloride on Lipolysis[*]

A. H. Kissebah , B. R. Tulloch , N.  Vydelingum , H.  Hope-Gill , P.  Clarke , T. R. Fraser
  • Endocrine Unit, Department of Medicine, Royal Postgraduate Medical School, London, England
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Publication Date:
07 January 2009 (online)

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Abstract

The effects of insulin and procaine-hydrochloride on the protein kinase activation ratio and the phosphatase(s) activity have been studied in rat adipose tissue. The relationship between the changes in the activities of these enzyme preparations and tissue cyclic-AMP has also been investigated.

Insulin decreased the protein kinase activation ratio and increased the phosphatase(s) activity prior to its effect on lipolysis. Such changes were not associated with comparable alterations in tissue cyclic-AMP. The changes in protein kinase and phosphatase activities correlated both in time and magnitude with the inhibition of lipolysis.

Procaine hydrochloride did not affect the tissue accumulation of cyclic-AMP in response to adrenalin, yet it produced similar changes to insulin in affecting the protein kinase activation ratio, phosphatase(s) activity and inhibition of lipolysis.

These results suggest a common mechanism for the anti-lipolytic effect of insulin and procaine which involves an alteration in cellular calcium distribution and is independent of their effects on tissue cyclic-AMP.

Key words

Cyclic-AMP - Adipose Tissue - Insulin - Procaine HCl - Glycerol Release - Adrenalin - Lipolysis

1 Presented in part to the British Diabetic Association, Spring meeting 13-14 April 1973.

1 Presented in part to the British Diabetic Association, Spring meeting 13-14 April 1973.

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