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Planta Med 2009; 75(13): 1405-1409
DOI: 10.1055/s-0029-1185718
Pharmacology
Original Paper
© Georg Thieme Verlag KG Stuttgart · New York

An Extract from Berries of Aronia melanocarpa Modulates the Generation of Superoxide Anion Radicals in Blood Platelets from Breast Cancer Patients

Magdalena Kedzierska1 , Beata Olas1 , Barbara Wachowicz1 , Anna Stochmal2 , Wieslaw Oleszek2 , Arkadiusz Jeziorski3 , Janusz Piekarski3 , Rafal Glowacki4
  • 1Department of General Biochemistry, University of Lodz, Lodz, Poland
  • 2Department of Biochemistry, Institute of Soil Science and Plant Cultivation, State Research Institute, Pulawy, Poland
  • 3Department of Oncological Surgery, Medical University of Lodz, Lodz, Poland
  • 4Department of Environmental Chemistry, University of Lodz, Lodz, Poland
Further Information

Publication History

received January 20, 2009 revised April 1, 2009

accepted April 4, 2009

Publication Date:
14 May 2009 (online)

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Abstract

Plant antioxidants protect cells against oxidative stress. Because oxidative stress (measured by different biomarkers) is observed in breast cancer patients, the aim of this study was to establish the effects of a polyphenol-rich extract of Aronia melanocarpa (final concentration of 50 µg/mL, 5 min, 37 °C) on superoxide anion radicals (O2 − •) and glutathione (GSH) in platelets from patients with breast cancer and in a healthy group in vitro. Generation of O2 − • in platelets before and after incubation with the extract was measured by cytochrome c reduction. Using HPLC, we determined the level of glutathione in blood platelets. We observed a statistically significant increase of biomarkers of oxidative stress such as O2 − • and a decrease in GSH in platelets from patients with breast cancer compared with the healthy group. We showed that the extract from A. melanocarpa added to blood platelets significantly reduced the production of O2 − • in platelets not only from the healthy group but also from patients with breast cancer. Considering the data presented in this study, we have demonstrated the protective role of the extract from A. melanocarpa in patients with breast cancer in vitro.

Key words

Aronia melanocarpa (Rosaceae) - blood platelets - breast cancer - free radicals

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Beata Olas

Department of General Biochemistry
Institute of Biochemistry
University of Lodz

Banacha 12/16

90-237 Lodz

Poland

Phone: + 48 4 26 35 44 84

Fax: + 48 4 26 35 44 84

Email: olasb@biol.uni.lodz.pl

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