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Int J Sports Med 2009; 30(7): 550-554
DOI: 10.1055/s-0029-1202342
Clinical Sciences

© Georg Thieme Verlag KG Stuttgart · New York

MMP-2 is not Altered By Stretching in Skeletal Muscle

S. M. Peviani 1 , A. R. S. Gomes 2 , H. S. Selistre de Araujo 3 , T. F. Salvini 1
  • 1Department of Physical Therapy, Federal University of São Carlos, São Carlos, Brazil
  • 2Department of Physical Therapy, Federal University of Paraná, Matinhos, Brazil
  • 3Department of Physiology, Federal University of São Carlos, São Carlos, Brazil
Further Information

Publication History

accepted after revision January 10, 2009

Publication Date:
19 March 2009 (online)

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Abstract

Considering that short bouts of stretching, as recommended in rehabilitation and sports activities, induce skeletal muscle and connective tissue adaptation, the hypothesis of this study was that MMP-2 activity is regulated by muscle stretch. The level of MMP-2 activity was, thereby, assessed after stretching in rat soleus muscle. Animals received a single session of stretching (10 stretches lasting 1 min each with 30 s of rest in between) and were evaluated immediately and after 8, 24, 48, 72 and 168 h. To evaluate the effect of repetitive sessions of stretching, three groups of animals were evaluated – one group after 2 sessions, another after 3, and a third after 7. MMP-2 activity was evaluated by zymography and MMP-2 mRNA was assessed by real-time polymerase chain reaction. None of the groups presented MMP-9 activity. MMP-2 activity and mRNA expression did not change after either single or repetitive sessions of stretching. In conclusion, the results of this study indicate that MMP-2 is not involved in the muscle stretch-induced remodeling.

Key words

stretching - MMP-2 - sports activities - rehabilitation - muscle adaptation - MMP-9

References

  • 1 Ahtikoski AM, Koskinen SO, Virtanen P, Kovanen V, Risteli J, Takala TE. Synthesis and degradation of type IV collagen in rat skeletal muscle during immobilization in shortened and lengthened positions.  Acta Physiol Scand. 2003;  177 473-481
    CrossrefPubMedGoogle Scholar
  • 2 Ansved T. Effects of immobilization on the rat soleus muscle in relation to age.  Acta Physiol Scand. 1995;  154 291-302
    CrossrefPubMedGoogle Scholar
  • 3 Bandy WD, Irion JM, Briggler M. The effect of time and frequency of static stretching on flexibility of the hamstring muscles.  Phys Ther. 1997;  77 1090-1096
    PubMedGoogle Scholar
  • 4 Bandy WD, Irion JM. The effects of time on static stretch on the flexibility of the hamstring muscle.  Phys Ther. 1994;  74 845-852
    PubMedGoogle Scholar
  • 5 Bloch RJ, Gonzalez-Serratos H. Lateral force transmission across costameres in skeletal muscle.  Exerc Sport Sci Rev. 2003;  31 73-78
    CrossrefPubMedGoogle Scholar
  • 6 Brown MD, Hudlicka O. Modulation of physiological angiogenesis in skeletal muscle by mechanical forces: involvement of VEGF and metalloproteinases.  Angiogenesis. 2003;  6 1-14
    CrossrefPubMedGoogle Scholar
  • 7 Carmeli E, Moas M, Lennon S, Powers SK. High intensity exercise increases expression of matrix metalloproteinases in fast skeletal muscle fibres.  Exp Physiol. 2005;  90 613-619
    CrossrefPubMedGoogle Scholar
  • 8 Carmeli E, Moas M, Reznick AZ, Coleman R. Matrix metalloproteinases and skeletal muscle: a brief review.  Muscle Nerve. 2004;  29 191-197
    CrossrefPubMedGoogle Scholar
  • 9 Carvalho RF, Dariolli R, Justulin  Jr  LA, Sugizaki MM, Okoshi MP, Cicogna AC, Felisbino SL, Silva MDP. Heart failure alters matrix metalloproteinase gene expression and activity in rat skeletal muscle.  Int J Exp Path. 2006;  87 437-443
    CrossrefPubMedGoogle Scholar
  • 10 Chang C, Werb Z. The many faces of metalloproteases: cell growth, invasion, angiogenesis and metastasis.  Trends Cell Biol. 2001;  11 37-43
    PubMedGoogle Scholar
  • 11 Chiquet M. Regulation of extracellular matrix gene expression by mechanical stress.  Matrix Biol. 1999;  18 417-426
    CrossrefPubMedGoogle Scholar
  • 12 Coutinho EL, Gomes AR, Franca CN, Oishi J, Salvini TF. Effect of passive stretching on the immobilized soleus muscle fiber morphology.  Braz J Med Biol Res. 2004;  37 1853-1861
    PubMedGoogle Scholar
  • 13 Coutinho EL, DeLuca C, Salvini TF, Vidal BC. Bouts of passive stretching after immobilization of the rat soleus muscle increase collagen macromolecular organization and muscle fiber area.  Connect Tissue Res. 2006;  47 278-286
    CrossrefPubMedGoogle Scholar
  • 14 De Deyne P. Application of passive stretch and its implications for muscle fibers.  Phys Ther. 2001;  81 819-826
    PubMedGoogle Scholar
  • 15 Demestre M, Orth M, Wells GM, Gearing AJ, Hughes RAC, Gregson NA. Characterization of matrix metalloproteinases in denervated muscle.  Neuropathol Appl Neurobiol. 2005;  31 545-555
    CrossrefPubMedGoogle Scholar
  • 16 Flück M, Hoppeler H. Molecular basis of skeletal muscle plasticity-from gene to form and function.  Rev Physiol Biochem Pharmacol. 2003;  146 159-216
    PubMedGoogle Scholar
  • 17 Gajdosik RL. Passive extensibility of skeletal muscle: review of the literature with clinical implications.  Clin Biomech. 2001;  16 87-101
    CrossrefPubMedGoogle Scholar
  • 18 Goldspink G, Scutt A, Loughna PT, Wells DJ, Jaencke T, Gerlach GF. Gene expression in skeletal muscle in response to strech and force generation.  Am J Physiol. 1992;  22 356-363
    PubMedGoogle Scholar
  • 19 Goldspink G, Williams P, Simpson H. Gene expression in response to muscle stretch.  Clin Orthop Relat Res. 2002;  1 146-152
    PubMedGoogle Scholar
  • 20 Gomes AR, Cornachione A, Salvini TF, Mattiello-Sverzut AC. Morphological effects of two protocols of passive stretch over the immobilized rat soleus muscle.  J Anat. 2007;  210 328-335
    CrossrefPubMedGoogle Scholar
  • 21 Gomes ARS, Coutinho EL, França CN, Polonio J, Salvini TF. The effect of one stretch a week applied to the immobilized soleus muscle on rat muscle fiber morphology.  Braz J Med Biol Res. 2004;  37 1473-1480
    PubMedGoogle Scholar
  • 22 Gomes ARS, Soares AG, Peviani SM, Nascimento RB, Moriscot AS, Salvini TF. The effect of 30 min of passive stretch of the rat soleus muscle on the myogenic differentiation, myostatin and atrogin-1 gene expressions.  Arch Phys Med Rehabil. 2006;  87 241-246
    CrossrefPubMedGoogle Scholar
  • 23 Harjola VP, Jankala H, Harkonen M. Myosin heavy chain mRNA and protein distribution in immobilized rat skeletal muscle are not affected by testosterone status.  Acta Physiol Scand. 2000;  169 277-282
    CrossrefPubMedGoogle Scholar
  • 24 Hwang JH, Ra YJ, Lee KM, Lee JY, Ghil SH. Therapeutic effect of passive mobilization exercise on improvement of muscle regeneration and prevention of fibrosis after laceration injury of rat.  Arch Phys Med Rehabil. 2006;  87 20-26
    PubMedGoogle Scholar
  • 25 Kherif S, Lafuma C, Dehaupas M, Lachkar S, Fournier JG, Verdiere-Sahuque M, Fardeau M, Alameddine HS. Expression of matrix metalloproteinases 2 and 9 in regenerating skeletal muscle: a study in experimentally injured and mdx muscles.  Dev Biol. 1999;  205 158-170
    CrossrefPubMedGoogle Scholar
  • 26 Koskinen SO, Ahtikoski AM, Komulainen J, Hesselink MK, Drost MR, Takala TE. Short-term effects of forced eccentric contractions on collagen synthesis and degradation in rat skeletal muscle.  Pflugers Arch. 2002;  444 59-72
    CrossrefPubMedGoogle Scholar
  • 27 Koskinen SO, Wang W, Ahtikoski AM, Kjaer M, Han XY, Komulainen J, Kovanen V, Takala TE. Acute exercise induced changes in rat skeletal muscle mRNAs and proteins regulating type IV collagen content.  Am J Physiol. 2001;  280 R1292-R1300
    PubMedGoogle Scholar
  • 28 Marqueti RC, Parizotto NA, Chriguer RS, Perez SEA, Selistre-de-Araujo HS. Androgenic-anabolic steroids associated with mechanical loading inhibit matrix metallopepetidase activity and affect the remodeling of the Achilles tendon in rats.  Am J Sports Med. 2006;  34 1274-1280
    CrossrefPubMedGoogle Scholar
  • 29 Menon B, Singh M, Singh K. Matrix metalloproteinases mediate beta-adrenergic receptor-stimulated apoptosis in adult rat ventricular myocytes.  Am J Physiol. 2005;  289 C168-C176
    CrossrefPubMedGoogle Scholar
  • 30 Nagase H, Woessner  Jr  JF. Matrix metalloproteinases.  J Biol Chem. 1999;  274 21491-21494
    CrossrefPubMedGoogle Scholar
  • 31 Page-McCaw A, Ewald AJ, Werb Z. Matrix metalloproteinases and the regulation of tissue remodeling.  Nat Rev Mol Cell Biol. 2007;  8 221-233
    CrossrefPubMedGoogle Scholar
  • 32 Peviani SM, Gomes ARS, Moreira RFC, Moriscot AS, Salvini TF. Short bouts of stretching increase Myo-D, Myostatin and Atrogin-1 in rat soleus muscle.  Muscle Nerve. 2007;  35 363-370
    CrossrefPubMedGoogle Scholar
  • 33 Stamenkovic I. Matrix metalloproteinases in tumor invasion and metastasis.  Semin Cancer Biol. 2000;  10 415-433
    CrossrefPubMedGoogle Scholar
  • 34 Stauber WT, Miller GR, Grimmett JG, Knack KK. Adaptation of rat soleus muscles to 4 wk of intermittent strain.  J Appl Physiol. 1994;  77 58-62
    PubMedGoogle Scholar
  • 35 Vu TH, Werb Z. Matrix metalloproteinases: effectors of development and normal physiology.  Genes Dev. 2000;  14 2123-2133
    CrossrefPubMedGoogle Scholar
  • 36 Williams PE. Effect of intermittent stretch on immobilized muscle.  Ann Rheum Dis. 1988;  47 1014-1016
    CrossrefPubMedGoogle Scholar

Correspondence

S. M. Peviani

Department of Physical Therapy

Federal University of São Carlos

Washington Luis

km 235

13565-905 São Carlos

Brazil

Phone: +55/16/3351 83 45

Fax: +55/16/3351 20 81

Email: sabrinapeviani@gmail.com

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