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Horm Metab Res 2011; 43(5): 331-336
DOI: 10.1055/s-0030-1269907
Original Basic

© Georg Thieme Verlag KG Stuttgart · New York

Effects of All-trans Retinoic Acid on Sodium/Iodide Symporter and CCAAT/Enhancer-binding Protein-Homologous Protein under Condition of Endoplasmic Reticulum Stress in FRTL5 Thyroid Cells

S. J. Lee1 , S. H. Kim1 , J. G. Kang1 , C. S. Kim1 , S.-H. Ihm1 , M. G. Choi1 , H. J. Yoo1
  • 1Division of Endocrinology and Metabolism, Department of Internal Medicine, College of Medicine, Hallym University, Chuncheon, Republic of Korea
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Publication History

received 11.08.2010

accepted 23.11.2010

Publication Date:
10 January 2011 (online)

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Abstract

In thyroid cells, the effects of all-trans retinoic acid (ATRA) on sodium/iodide symporter (NIS) and CCAAT/enhancer-binding protein-homologous protein (CHOP) under condition of endoplasmic reticulum (ER) stress have not been evaluated. In the present study, the relationships between NIS, CHOP, and p38 MAPK, and the effects of ATRA on NIS and CHOP expression as well as on p38 MAPK activation under condition of ER stress in thyroid cells were investigated. In FRTL5 thyroid cells, NIS mRNA and protein levels decreased following tunicamycin (TN) treatment, while CHOP mRNA and protein levels increased. In addition, while CHOP mRNA levels decreased after administration of tauro-UDCA and siCHOP, NIS mRNA levels were not altered. After pretreatment with SB203580, NIS mRNA levels decreased in non-TN-treated cells but increased in TN-treated cells. In contrast, CHOP mRNA levels decreased in both non-TN-treated and TN-treated cells. Exposure to ATRA decreased NIS mRNA levels in non-TN-treated cells but increased NIS mRNA levels in TN-treated cells. ATRA decreased CHOP mRNA levels in both non-TN-treated and TN-treated cells although the response was significant only in TN-treated cells. Phospho-p38 MAPK protein levels but not total p38 MAPK protein levels increased in TN-treated cells. ATRA attenuated this increase in phopho-p38 MAPK protein levels. In conclusion, our results demonstrate that ER stress may induce reciprocal changes in NIS and CHOP expression via p38 MAPK in FRTL5 thyroid cells, and that ATRA may attenuate ER stress-induced alterations in NIS and CHOP expression by modulating the phosphorylation of p38 MAPK in FRTL5 thyroid cells.

Key words

all-trans retinoic acid - sodium/iodide symporter - CCAAT/enhancer-binding protein-homologous protein - p38 MAPK - endoplasmic reticulum stress

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Correspondence

Prof. S. J. LeeMD, PhD 

Division of Endocrinology and

Metabolism

Department of Internal

Medicine

College of Medicine

Hallym University

200-704 Chuncheon

Republic of Korea

Phone: +82/31/380 3700

Fax: +82/31/383 3768

Email: leesj@hallym.ac.kr

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