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Int J Sports Med 2011; 32(4): 247-253
DOI: 10.1055/s-0030-1270513
Physiology & Biochemistry

© Georg Thieme Verlag KG Stuttgart · New York

Extracellular Matrix and Myofibrils During Unloading and Reloading of Skeletal Muscle

P. Kaasik1 , E.-M. Riso2 , T. Seene2
  • 1Department of Functional Morphology, University of Tartu, Estonia
  • 2Institute of Exercise Biology and Physiotherapy, University of Tartu, Estonia
Further Information

Publication History

accepted after revision December 13, 2010

Publication Date:
04 March 2011 (online)

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Abstract

The aim of the study was to elucidate the effect of unloading and reloading on the collagen expression and synthesis rate of myofibrillar proteins in fast-twitch (FT) muscle in relation to changes in muscle strength and motor activity. Northern blot analysis was used for testing the specificity of cDNA probes and protein synthesis rate was measured according to incorporation of radioactive leucine into different protein fractions. Unloading depresses collagen type I and III (p<0.001), type IV (p<0.05) and reloading enhances collagen expression in fast-twitch skeletal muscle in comparison with unloading. Enhanced expression of matrix metalloproteinase-2 continued during the first week of reloading (p<0.01) and tissue inhibitor of metalloproteinase-2 during reloading (p<0.05). Changes in collagen expression in FT muscle are in good agreement with changes in myofibrillar protein synthesis during unloading and reloading. In conclusion alterations in extracellular matrix and myofibrillar apparatus in FT skeletal muscle are related to changes in muscle strength and motor activity, are significant in exercise training and determination of recovery periods in the training process as well as in athletes’ rehabilitation.

Key words

collagens - myofibrillar proteins - unloading - reloading

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Correspondence

Prof. Teet SeenePhD, Dsci 

University of Tartu

Institute of Exercise Biology and

Physiotherapy

Ülikooli 18

50090 Tartu

Estonia

Phone: +372/7/375 364

Fax: +372/7/375 379

Email: teet.seene@ut.ee

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