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Horm Metab Res 2011; 43(3): 165-170
DOI: 10.1055/s-0030-1270526
Original Basic

© Georg Thieme Verlag KG Stuttgart · New York

Phosphoproteomic Identification of a PDX-1/14-3-3ε Interaction in Pancreatic Beta Cells

P. Ziegler1 , 2 , [*] , S. Teller1 , [*] , N. H. Ha1 , B. Giese3 , M. Fraunholz3 , R. Walther1
  • 1Department of Medical Biochemistry and Molecular Biology, University of Greifswald, Greifswald, Germany
  • 2Medical Clinic IV, University Hospital of the RWTH Aachen, Aachen, Germany
  • 3Bioinformatics/Toponomics, Competence Center for Functional Genomics, Institute for Microbiology, University of Greifswald, Greifswald, Germany
Further Information

Publication History

received 09.09.2010

accepted 24.12.2010

Publication Date:
01 February 2011 (online)

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Abstract

Glucose-dependent activation of the homeodomain transcription factor PDX-1 leads to its phosphorylation, to an increase in DNA binding capacity, and to NLS dependent translocation into the nucleus. To uncover unknown mediators of PDX-1 activation, PDX-1 interacting proteins were analysed by pull-down from 32P-labelled, glucose-stimulated MIN6 cells. Recovered proteins were analysed by 2D gel electrophoresis and mass spectrometry. We identified 14-3-3ε as a novel PDX-1 binding protein and confirmed the interaction in vivo by Fluorescence Resonance Energy Transfer (FRET) analysis. We propose that 14-3-3ε interacts directly with PDX-1 to regulate its cellular distribution in pancreatic beta cells.

Key words

PDX-1 - 14-3-3ε - 2 dimensional gel electrophoresis - beta cell

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1 These authors contributed equally to the work.

Correspondence

Prof. Dr. R. Walther

Department of Medical

Biochemistry and Molecular

Biology

Sauerbruchstraße

17487 Greifswald

Germany

Phone: +49/3834/865 400

Fax: +49/3834/865 402

Email: rwalther@uni-greifswald.de

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