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Planta Med 2011; 77(13): 1495-1503
DOI: 10.1055/s-0030-1270783
Biological and Pharmacological Activity
Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

Differential Inhibitory Effects of Salvianolic Acids on Activation of Rat Hepatic Stellate Cells by Platelet-Derived Growth Factor

Ming-Kuei Tsai1 , Yun-Lian Lin2 , Yi-Tsau Huang1 , 2
  • 1Institute of Traditional Medicine, National Yang-Ming University, Taipei, Taiwan
  • 2National Research Institute of Chinese Medicine, Taipei, Taiwan
Further Information

Publication History

received October 22, 2010 revised Dec. 27, 2010

accepted January 21, 2011

Publication Date:
21 February 2011 (online)

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Abstract

Platelet-derived growth factor (PDGF) induces cell proliferation together with oxidative stress. The present study investigated the effects of salvianolic acid A (Sal A) and B (Sal B) on the PDGF-induced signaling cascades in hepatic stellate cells (HSCs). HSC-T6, a rat hepatic stellate cell line, was stimulated with PDGF (10 ng/mL). The inhibitory effects of Sal A and B on oxidative stress-related signaling pathways were assessed in vitro. The protein levels were measured by Western blotting. FACS analysis was applied to detect the thioredoxin (Trx) level. Sal A and B showed different inhibitory abilities on the PDGF-related pathway. Sal A inhibited 70-kDa ribosomal S6 kinase (p70s6k) and associated proteins. Sal B attenuated PDGF-induced c-jun-N-terminal kinase (JNK), p38, and PKC-δ phosphorylations. Both Sal A and B diminished the activation of PKD, Trx, heme-oxygenase (HO)-1, and Nrf2. Taken together, our results showed that Sal A and B attenuated PDGF-induced ROS formation in HSCs, possibly through different signaling pathways.

Key words

hepatic stellate cells - salvianolic acid - platelet-derived growth factor - oxidative stress - hepatic fibrosis - Salvia miltiorrhiza - Lamiaceae

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Dr. Yi-Tsau Huang, MD, PhD

Institute of Traditional Medicine
National Yang-Ming University

155, Li-Nong Street, Section 2

Taipei 112

Taiwan

Phone: +88 62 28 26 71 79

Fax: +88 62 28 22 50 44

Email: huangyt@ym.edu.tw

Dr. Yun-Lian Lin

Division of Medicinal Chemistry
National Research Institute of Chinese Medicine

155-1, Li-Nong Street, Section 2

Taipei 112

Taiwan

Phone: +88 62 28 22 55 92

Fax: +88 62 28 23 04 88

Email: yllin@nricm.edu.tw

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