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Planta Med 2011; 77(13): 1489-1494
DOI: 10.1055/s-0030-1270886
Biological and Pharmacological Activity
Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

Antimutagenic Potential of Solanum lycocarpum against Induction of Chromosomal Aberrations in V79 Cells and Micronuclei in Mice by Doxorubicin

Denise Crispim Tavares1 , Carla Carolina Munari1 , Marcelo Gonzaga de Freitas Araújo1 , Mariana Correa Beltrame1 , Michelle Andrade Furtado1 , Camila Costa Gonçalves1 , Renata Fabiane Jorge Tiossi2 , Jairo Kenupp Bastos2 , Wilson Roberto Cunha1 , Rodrigo Cassio Sola Veneziani1
  • 1Núcleo de Pesquisas em Ciências Exatas e Tecnológicas, Universidade de Franca, Franca, SP, Brazil
  • 2Laboratório de Farmacognosia, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil
Further Information

Publication History

received October 6, 2010 revised February 3, 2011

accepted February 9, 2011

Publication Date:
07 March 2011 (online)

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Erratum for: Antimutagenic Potential of Solanum lycocarpum against Induction of Chromosomal Aberrations in V79 Cells and Micronuclei in Mice by DoxorubicinPlanta Med 2011; 77(13): E13-E13
DOI: 10.1055/s-0031-1280192

Abstract

Solanum lycocarpum A. St. Hil. (Solanaceae) is a hairy shrub or small much-branched tree of the Brazilian Cerrado. S. lycocarpum fruits are commonly used in traditional medicine in powder form or as folk preparations for the treatment of diabetes and obesity, as well as for controlling cholesterol levels. The aim of the present study was to chemically characterize the hydroalcoholic extract (SL) of S. lycocarpum by determination of total flavonoids and total poyphenols and quantification of steroidal alkaloids, as well as to evaluate its mutagenic and/or antimutagenic potential on V79 cells and Swiss mice using chromosomal aberrations and bone marrow micronucleus assays, respectively. Three concentrations of SL (16, 32, and 24 µg/mL) were used for the evaluation of its mutagenic potential in V79 cells and four doses (0.25, 0.50, 1.0, and 2.0 g/kg body weight) were used for Swiss mice. In the antimutagenicity assays, the different concentrations of SL were combined with the chemotherapeutic agent doxorubicin (DXR). HPLC analysis of SL gave contents of 6.57 % ± 0.41 of solasonine and 4.60 % ± 0.40 of solamargine. Total flavonoids and polyphenols contents in SL were 0.04 and 3.60 %, respectively. The results showed that not only SL exerted no mutagenic effect, but it also significantly reduced the frequency of chromosomal aberrations induced by DXR in both V79 cells and micronuclei in Swiss mice at the doses tested.

Key words

Solanum lycocarpum A. St. Hil. - Solanaceae - steroid alkaloids - V79 cells - bone marrow

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Rodrigo Cassio Sola Veneziani

Núcleo de Pesquisas em Ciências Exatas e Tecnológicas
Universidade de Franca

Av. Dr. Armando Salles de Oliveira 201

Franca 14404-600 SP

Brazil

Phone: +55 16 37 11 88 71

Fax: +55 16 37 11 88 78

Email: rcsvenez@unifran.br

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