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Horm Metab Res 2011; 43(7): 452-457
DOI: 10.1055/s-0031-1277187
Original Basic

© Georg Thieme Verlag KG Stuttgart · New York

Supraphysiological Triiodothyronine Doses Diminish Leptin and Adiponectin Gene Expression, but do not Alter Resistin Expression in Calorie Restricted Obese Rats

R. A. M. Luvizotto1 , M. T. Síbio1 , R. M. C. Olímpio1 , A. F. Nascimento1 , A. P. Lima-Leopoldo2 , A. S. Leopoldo1 , 2 , C. R. Padovani3 , A. C. Cicogna1 , C. R. Nogueira1
  • 1Department of Clinical Medicine, Botucatu School of Medicine, UNESP – University of São Paulo State, Botucatu, São Paulo, Brazil
  • 2Departament of Sports, Center of Physical Education and Sports, UFES – Federal University of Espirito Santo, Vitória, Espírito Santo, Brazil
  • 3Department of Biostatistics, Institute of Biological Sciences, UNESP – University of São Paulo State, Botucatu, São Paulo, Brazil
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Publication History

received 08.12.2010

accepted 12.04.2011

Publication Date:
09 May 2011 (online)

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Abstract

Thyroid hormones regulate energy balance and act on adipokines. However, while it is unclear what the effects are of calorie restriction and high doses of triiodothyronine (T3) on adipokines in obesity, thyroid hormones are illicitly administered in isolation or in association with a hypocaloric diet as an obesity treatment. The present study determined the effect of T3 on serum concentrations and gene expression of the adipokines leptin, resistin, and adiponectin in calorie-restricted obese rats. Male Wistar rats received a hypercaloric diet for 20 weeks followed by calorie restriction for 8 weeks. The animals were then randomly divided into 3 groups: calorie restriction (OR), OR with 5 μg of T3/100 g BW (RS1), and OR with 25 μg of T3/100 g BW (RS2) for 2 weeks. Blood and adipose tissue samples were collected for biochemical, hormonal, and gene expression analyses. Serum concentrations of leptin (OR: 3.7±0.6, RS1: 3.8±1, RS2 0.2±0.07 ng/dl) and resistin (OR: 2.5±0.6, RS1: 2.5±0.5, RS2 1.6±0.3 ng/dl) were diminished at the higher dose, while serum adiponectin (OR: 31±7, RS1: 24±5, RS2 26±7 ng/dl) levels were lower in the low dose group. Administration of T3 reduced leptin gene expression (OR: 0.91±0.1, RS1: 0.95±0.1, RS2 0.22±0.1) only at the higher dose, resistin expression (OR: 1.06±0.2, RS1: 1.04±0.1, RS2 0.88±0.2) was not influenced by T3 treatment, and adiponectin expression (OR: 1.55±0.5, RS1: 0.95±0.15, RS2 0.97±0.13) was diminished independent of the T3 dose. These results indicate that T3, directly or indirectly, inhibits the expression of leptin and adiponectin in calorie restricted obese animals.

Key words

thyroid hormone - adipose tissue - high-fat diet - food deprivation

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Correspondence

R. A. M. Luvizotto

Botucatu School of Medicine

University of São Paulo State

– UNESP

Distrito de Rubião Jr s/n

Botucatu SP CEP: 18618-000

Brazil

Phone: +55/14/3881 6213

Fax: +55/14/3881 6424

Email: reluvizotto@yahoo.com

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