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Arzneimittelforschung 2001; 51(8): 633-637
DOI: 10.1055/s-0031-1300093
Antioxidants
Editio Cantor Verlag Aulendorf (Germany)

Assessment of Antioxidant Activity of Some Antileprotic Drugs

Talluri Kalpana
Drug Metabolism and Clinical Pharmacokinetics Division, University College of Pharmaceutical Sciences, Kakatiya University, Warangal A.P., India
,
Neelam Karunakar
Drug Metabolism and Clinical Pharmacokinetics Division, University College of Pharmaceutical Sciences, Kakatiya University, Warangal A.P., India
,
Mada S. Reddy
Drug Metabolism and Clinical Pharmacokinetics Division, University College of Pharmaceutical Sciences, Kakatiya University, Warangal A.P., India
,
Marsanapalle C. Prabhakar
Drug Metabolism and Clinical Pharmacokinetics Division, University College of Pharmaceutical Sciences, Kakatiya University, Warangal A.P., India
,
Devarakonda R. Krishna
Drug Metabolism and Clinical Pharmacokinetics Division, University College of Pharmaceutical Sciences, Kakatiya University, Warangal A.P., India
› Author Affiliations
Further Information

Publication History

Publication Date:
26 December 2011 (online)

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Summary

DPPH (α,α-diphenyl-β-picryl hydrazyl) (CAS 217-591-8), a stable free radical can be used to determine the antioxidant activity (AOA) of some drugs. In the present study the DPPH method was used for the first time to test AOA of dapsone (CAS 80-08-0), clofazimine (CAS 2030-63-9) and rifampicin (CAS 13282-42-1) in vitro and deproteinated blood method. Ascorbic acid (CAS 50-81-7) was used as a control in the study, which showed concentration-dependent antioxidant activity. Rifampicin showed a per se effect but it showed concentration dependent decrease in the DPPH absorbance. Ascorbic acid, dapsone and rifampicin showed DPPH scavenging activity both in vitro and deproteinated blood method. Clofazimine did not have any influence on DPPH. This method may be extended to different drugs for testing their AOA in biological fluids.

Zusammenfassung

Bewertung der Aktivität von Antileprotika als Antioxidantien

DPPH (α,α-Diphenyl-β-picryl-hydrazyl) (CAS 217-591-8), ein stabiles freies Radikal, wird zur Bestimmung des antioxidativen Aktivität (AOA) einiger Arzneimittel benutzt. In der vorliegenden Studie wurde die DPPH-Methode erstmals zur Bewertung der AOA von Dapson (CAS 80-08-0), Clofazimin (CAS 2030-63-9) und Rifampicin (CAS 13282-46-1) in proteinfreiem Blut eingesetzt. Ascorbinsäure (CAS 50-81-7) diente als Kontrolle. Rifampicin hatte einen per se-Effekt, aber auch eine konzentrationsabhängige Wirksamkeit. Ascorbinsäure, Dapson und Rifampicin waren in vitro wie auch im proteinfreien Blut wirksam. Clofazimin hatte keinen Einfluß auf die DPPH-Aktivität. Die Methode ist auch für die Analyse anderer Medikamente geeignet.

Key words

Antileprotic s, antioxidant activity, DPPH method - Ascorbic acid - Clofazimine - Dapsone - Rifampicin