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Neuropediatrics 2012; 43(06): 320-331
DOI: 10.1055/s-0032-1325121
Original Article
Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

The Effect of Recombinant Stromal Cell-Derived Factor-1 Treatment on Hypoxic-Ischemic Brain Injury in Neonatal Mice

WeiWei Zhu
1   Department of Pediatrics, Jinan Central Hospital affiliated to Shandong University, Jinan, China
,
XiaoLi Ma
2   Central Laboratory, Jinan Central Hospital affiliated to Shandong University, Jinan, China
,
Feng Li
1   Department of Pediatrics, Jinan Central Hospital affiliated to Shandong University, Jinan, China
,
Jun Wang
1   Department of Pediatrics, Jinan Central Hospital affiliated to Shandong University, Jinan, China
,
LiChun Yu
3   Department of Pediatrics, Provincial Hospital affiliated to Shandong University, Jinan, China
,
Mi Xu
1   Department of Pediatrics, Jinan Central Hospital affiliated to Shandong University, Jinan, China
,
AiHua Ma
3   Department of Pediatrics, Provincial Hospital affiliated to Shandong University, Jinan, China
,
AiLi Guo
1   Department of Pediatrics, Jinan Central Hospital affiliated to Shandong University, Jinan, China
,
Nan Zhang
1   Department of Pediatrics, Jinan Central Hospital affiliated to Shandong University, Jinan, China
› Author Affiliations
Further Information

Publication History

16 November 2011

20 July 2012

Publication Date:
08 October 2012 (online)

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Abstract

Background Stromal cell-derived factor-1 (SDF-1) plays a critical role in adult brain injury repair including neurogenesis and vasculogenesis. However, the effects of recombinant SDF-1(rSDF-1) treatment on hypoxic-ischemic (HI) brain injury in neonatal mice are not clear.

Materials and Methods Seven-day-old (P7) C57BL/6J mice were divided into sham group, control group, and rSDF-1 group. Mice brains were collected to determine histopathological damage and the expression level of SDF-1, caspase-3, and Ki67 on P8, P10, P14, and P21. Passive avoidance and elevated plus-maze tests were also assessed on P20 and P21.

Results Compared with control group, rSDF-1 treatment increased the weight ratio of left and right brain hemisphere (p < 0.05), reduced the number of electric foot shocks (p < 0.05) and the percentage of time spent in the open arms (p < 0.05), meanwhile, increased the retention latency (p < 0.05) and the percentage of time spent in the enclosed arms (p < 0.05) on P20 and P21. High expression of Ki67 and low expression of caspase-3 were also observed.

Discussion This study showed that rSDF-1 treatment effectively alleviated brain injury shown by reducing in caspase-3 expression and increasing in Ki67 expression. Moreover, rSDF-1 treatment significantly improved behavioral performances in juvenile mice after HI.

Keywords

hypoxia-ischemia brain injury - stromal cell-derived factor-1 - apoptosis - proliferation - functional recovery - neonatal mice
 

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