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Z Gastroenterol 2014; 52(2): 180-186
DOI: 10.1055/s-0033-1336007
Originalarbeit
© Georg Thieme Verlag KG Stuttgart · New York

Alteration of Predominant Gastrointestinal Flora and Oxidative Damage of Large Intestine Under Simulated Hypobaric Hypoxia

Veränderung der vorherrschenden Mikroflora des Gastrointestinaltrakts und oxidative Schädigung des Dickdarms bei simulierter hypobarer Hypoxie
A. Adak
,
C. Maity
,
K. Ghosh
,
K. C. Mondal
Further Information

Publication History

04 January 2013

06 June 2013

Publication Date:
13 February 2014 (online)

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Abstract

Hypobaric hypoxia is an immediate and crucial starting mechanism of acute mountain sickness included with some non-specific gastrointestinal (GI) complications. To study the effect of hypoxia on GI microflora and its upshot to this system, male albino rats were exposed to 55 kPa (air pressure ~ 4872.9 m altitude) consecutively 30 days for 8 hours/day. The different indicator group of large intestinal microbial populations were enumerated and correlated with the levels of antioxidant indicators like catalase (CAT), superoxide dismutase (SOD), malondialdehyde (MDA), reduced glutathione (GSH) and oxidized glutathione (GSSG) of large intestinal epithelial cells. In addition, the histological study was performed by haematoxylin eosin (HE), periodic acid schiff staining (PAS) and scanning electron microscopy (SEM). It was observed that the density of total aerobes (104 folds) significantly (p < 0.05) decreased but the population of total anaerobes (209 folds) and Escherichia coli (125 folds) elevated after 30 days of hypoxic stress. The strict anaerobes like Bifidobacterium spp. (3 folds), Bacteroides spp. (134 folds), Lactobacillus spp. (7 folds) and other selected obligate anaerobes like Clostridium perfringens (40 folds), Peptostreptococcus spp. (21 folds) increased in respect to their control population. The growth direction index (GDI) of anaerobic populations was positive and correlated with gas formation aptitude. The activities of CAT and SOD in the large intestinal epithelia decreased significantly (p < 0.05) and GSH/GSSG pool turned into oxidized state with higher MDA (p < 0.05) formation. Histological study revealed the necrotized epithelial layer with higher lymphocytes infiltration in lamina propia accompanied by reduction of acidic mucins secreting goblet cells. From this experiment, it can be hypothesized that high altitude induced hypoxia manipulated the bacterial imprint and damaged the epithelial barrier of the large intestine which may cause systemic infection.

Zusammenfassung

Hypobare Hypoxie ist ein unmittelbar einsetzender und kritischer Startmechanismus der akuten Bergkrankheit, der mit einigen unspezifischen gastrointestinalen Komplikationen einhergeht. Um die Wirkung von Hypoxie auf die Mikroflora des Gastrointestinaltrakts und deren systemische Auswirkung zu untersuchen, wurden männliche Albinoratten an 30 aufeinanderfolgenden Tagen 8 Stunden pro Tag einem Druck von 55 kPa (Luftdruck entspricht 4872,9 Höhenmeter) ausgesetzt. Die unterschiedlichen populationsbezogenen Indikatoren der Dickdarmflora wurden quantifiziert und mit den Spiegeln der Antioxidant-Indikatoren wie Katalase (CAT), Superoxiddismutase (SOD), Malondialdehyd (MDA), reduziertem Glutathion (GSH) und oxidiertem Glutathion (GSSG) der Epithelzellen des Dickdarms korreliert. Zusätzlich wurde eine histologische Untersuchung mit Hämatoxylin-Eosin-Färbung (HE-Färbung), Perjodsäure-Schiff-Reaktion (PAS-Reaktion) und Rasterelektronen­mikroskopie durchgeführt. Die Untersuchungen ergaben, dass die Dichte aller Aeroben (104-fach) signifikant (p < 0,05) abnahm, während die Population aller Anaeroben (209-fach) und von Escherichia coli (125-fach) nach 30 Tagen hypoxischer Belastung zunahm. Strikte Anaerobe wie Bifidobacterium spp. (3-fach), Bacteroides spp. (134-fach), Lactobacillus spp. (7-fach) und andere ausgewählte obligate Anaerobe wie Clostridium perfringens (40 fach) und Peptostreptococcus spp.  (21-fach) vermehrten sich im Vergleich zu ihrer Kontrollpopulation. Der Wachstumsindex (growth direction index GDI) der anaeroben Population war positiv und korrelierte mit der Fähigkeit zur Gasbildung. Die Aktivität von CAT und SOD in den Epithelzellen des Dickdarms nahm signifikant ab (p < 0,05) und der GSH/GSSG Pool ging mit einer erhöhten MDA (p < 0,05) Bildung in den oxidierten Zustand über. Die histologische Untersuchung zeigte eine nekrotisierte Epithelschicht mit einer erhöhten Lymphozyten-Infiltration in der Lamina Propia, begleitet von einer Reduktion an saurem Mucin-bildenden Becherzellen. Dieser Versuch legt die These nahe, dass eine durch große Höhe verursachte Hypoxie die bakterielle Flora verändert und die Epithelbarriere des Dickdarms schädigt, was zu einer systemischen Infektion führen kann.

Key words

hypobaric hypoxia - acute mountain sickness - large intestine - histological study - systemic infection

Schlüsselwörter

hypobare Hypoxie - akute Bergkrankheit - Dickdarm - histologische Untersuchung - systemische Infektion
 

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