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J Reconstr Microsurg 2013; 29(09): 571-578
DOI: 10.1055/s-0033-1348037
Original Article
Thieme Medical Publishers 333 Seventh Avenue, New York, NY 10001, USA.

Remote Postconditioning Attenuates Ischemia/Reperfusion Injury in Rat Skeletal Muscle through Mitochondrial ATP-Sensitive K+ Channel–Dependent Mechanism

Jung Il Lee
1   Department of Orthopedic Surgery, Inje University Ilsan Paik Hospital, Goyang, Korea
,
Kyung Wook Nha
1   Department of Orthopedic Surgery, Inje University Ilsan Paik Hospital, Goyang, Korea
,
Jin Soo Suh
1   Department of Orthopedic Surgery, Inje University Ilsan Paik Hospital, Goyang, Korea
,
Suk Kyu Choo
1   Department of Orthopedic Surgery, Inje University Ilsan Paik Hospital, Goyang, Korea
,
Jung Ho Park
2   Department of Orthopedic Surgery, Korea University Ansan Hospital, Ansan, Korea
,
Jong Woong Park
2   Department of Orthopedic Surgery, Korea University Ansan Hospital, Ansan, Korea
› Author Affiliations
Further Information

Publication History

28 September 2012

29 March 2013

Publication Date:
11 June 2013 (online)

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Abstract

The authors investigated whether a remote postconditioning (remote post-con) procedure attenuated skeletal muscle ischemia/reperfusion (I/R) injury. We determined the optimal protocol of remote post-con and investigated its mechanism. Ischemia was induced for 3 hours in rat left hindlimb and three protocols of remote post-con were applied in right hindlimb just before the end of ischemia. The first (10-second group) involved two cycles of 10 seconds of occlusion followed by 10 seconds of reperfusion. The second (5-minute group) involved two cycles of 5 minutes of occlusion/reperfusion. The third (10-minute group) involved two cycles of 10 minutes of occlusion/reperfusion. In 5- and 10-minute groups, wet/dry ratio and muscle fiber edema were significantly lower than control group. Muscle contractility was preserved in 5- and 10-minute groups. An injection of 5-hydroxydecanoate (a specific blocker of mitochondrial ATP-sensitive K+ [mKATP] channels) impaired this effect. This study demonstrates that remote post-con preserves muscle contractility and reduces tissue edema and necrosis, possibly through the activation of mKATP channels. We suggest that two cycles of 5 minutes of occlusion followed by 5 minutes of reperfusion are optimal protocols of remote post-con in skeletal muscle I/R injury.

Keywords

Ischemia/reperfusion injury - postconditioning - remote postconditioning - ATP-sensitive K+ channel

Approval

The study protocol was approved by the Institutional Animal Care and Use Committee (Approval No. KUIACUC-20110914–1).


 

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