Download PDF
Planta Med 2013; 79(17): 1653-1655
DOI: 10.1055/s-0033-1351022
Biological and Pharmacological Activity
Letters
Georg Thieme Verlag KG Stuttgart · New York

In Vitro Antiparasitic Activity and Chemical Composition of the Essential Oil Obtained from the Fruits of Piper cubeba

Viviane Rodrigues Esperandim
1   University of Franca, Franca, São Paulo, Brazil
,
Daniele da Silva Ferreira
1   University of Franca, Franca, São Paulo, Brazil
,
Karen Cristina Sousa Rezende
1   University of Franca, Franca, São Paulo, Brazil
,
Lizandra Guidi Magalhães
1   University of Franca, Franca, São Paulo, Brazil
,
Julia Medeiros Souza
1   University of Franca, Franca, São Paulo, Brazil
,
Patrícia Mendonça Pauletti
1   University of Franca, Franca, São Paulo, Brazil
,
Ana Helena Januário
1   University of Franca, Franca, São Paulo, Brazil
,
Rosangela da Silva de Laurentz
2   College of Engineer of Ilha Solteira (UNESP), Ilha Solteira, São Paulo, Brazil
,
Jairo Kenupp Bastos
3   School of Pharmaceutical Sciences of Ribeirão Preto (USP), Ribeirão Preto, São Paulo, Brazil
,
Guilherme Venâncio Símaro
1   University of Franca, Franca, São Paulo, Brazil
,
Wilson Roberto Cunha
1   University of Franca, Franca, São Paulo, Brazil
,
Márcio Luis Andrade e Silva
1   University of Franca, Franca, São Paulo, Brazil
› Author Affiliations
Further Information

Publication History

received 27 June 2013
revised 02 October 2013

accepted 04 October 2013

Publication Date:
28 November 2013 (online)

    Permissions and Reprints

Abstract

Protozoans of the trypanosomatid family cause the neglected tropical diseases leishmaniasis and trypanosomiasis, for which few drugs are available. In this context our group has recently reported that the essential oil obtained by steam distillation of the fruits of Piper cubeba is active against Schistosoma mansoni. Therefore, we have investigated the in vitro effects of the essential oil against the trypomastigote and amastigote forms of Trypanosoma cruzi isolated from an LLCMK2 cell line culture and the promastigote forms of Leishmania amazonensis. The in vitro activity of the essential oil against trypomastigotes of T. cruzi increased upon rising concentrations, giving IC50 values of 45.5 and 87.9 µg · mL−1 against trypomastigotes and amastigotes, respectively. The essential oil was not active against L. amazonensis, since it displayed lyses of only 24 % at 400 µg · mL−1, and an IC50 of 326.5 µg · mL−1. Therefore, the essential oil should be further investigated to determine the compounds responsible for the observed activities, as well as its mechanism of action.

Key words

Piper cubeba - Piperaceae - essential oil - trypanocidal activity - leishmanicidal activity

Supporting Information

 

  • References

  • 1 Stuart K, Brun R, Croft S, Fairlamb A, Gürtler RE, McKerrow J, Reed S, Tarleton R. Kinetoplastids: related protozoan pathogens, different diseases. J Clin Invest 2008; 118: 1301-1310
    CrossrefPubMedGoogle Scholar
  • 2 Coura JR. Chagas disease: what is known and what is needed – a background article. Mem Inst Oswaldo Cruz 2007; 102: 113-122
    PubMedGoogle Scholar
  • 3 Schmidt TJ, Khalid SA, Romanha AJ, Alves TMA, Biavatti MW, Brun R, Da Costa FB, de Castro SL, Ferreira VF, de Lacerda MVG, Lago JHG, Leon LL, Lopes NP, das Neves Amorim RC, Niehues M, Ogungbe IV, Pohlit AM, Scotti MT, Setzer WN, Soeiro NC, Steinde M, Tempone AG. The potential of secondary metabolites from plants as drugs or leads against protozoan neglected diseases – part I. Cur Med Chem 2012; 19: 2176-2228
    PubMedGoogle Scholar
  • 4 Rocha MO, Teixeira MM, Ribeiro AL. An update on the management of Chagas cardiomyopathy. Exp Rev Antiinfect Ther 2007; 5: 727-743
    PubMedGoogle Scholar
  • 5 Rassi AJR, Rassi A, Marin-Neto JÁ. Chagas disease. Lancet 2010; 375: 1388-1402
    CrossrefPubMedGoogle Scholar
  • 6 Parker ER, Sethi A. Chagas disease: coming to a place near you. Dermatol Clin 2011; 29: 53-62
    CrossrefPubMedGoogle Scholar
  • 7 Raman VS, Duthie MS, Fox CB, Matlashewski G, Reed SG. Adjuvants for Leishmania vaccines: from models to clinical application. Front Immunol 2012; 3: 144
    PubMedGoogle Scholar
  • 8 WHO. Chagas disease (American trypanosomiasis) (2011). Available at http://www.who.int/mediacentre/factsheets/fs340/en/index.html Accessed January 12, 2013
    PubMedGoogle Scholar
  • 9 WHO. Leishmaniasis: the vector (2011). Available at http://www.who.int/leishmaniasis/en/index.html Accessed November 24, 2012
    PubMedGoogle Scholar
  • 10 Rezende KCS, Lucarini R, Símaro GV, Esperandim VR, Ferreira DS, Magalhães LG, Cunha WR, Bastos JK, Silva MLA. Leishmanicidal effect of derivatives of dibenzylbutirolactonic lignans from Piper cubeba . Planta Med 2012; 11: 78
    PubMedGoogle Scholar
  • 11 Hannaert V. Sleeping sickness pathogen (Trypanosoma brucei) and natural products: therapeutic targets and screening systems. Planta Med 2011; 77: 586-597
    Article in Thieme ConnectPubMedGoogle Scholar
  • 12 Magalhães LG, Souza JM, Wakabayashi KAL, Laurentiz R, Vinhólis AHC, Rezende KCS, Simaro GV, Bastos JK, Rodrigues V, Esperandim VR, Ferreira DS, Crotti AEM, Cunha WR, Silva MLA. In vitro efficacy of the essential oil of Piper cubeba L. (Piperaceae) against Schistosoma mansoni . Parasitol Res 2012; 110: 1747-1754
    CrossrefPubMedGoogle Scholar
  • 13 Pérez-Rebolledo A. 4-Nitroacetophenone-derived thiosemicarbazones and their copper (II) complexes with significant in vitro anti-trypanosomal activity. Eur J Med Chem 2008; 43: 939-948
    CrossrefPubMedGoogle Scholar
  • 14 Natera S, Machuca SC, Padroʼn-Nieves M, Romero A, Dıaz E, Ponte-Sucre A. Leishmania spp: proficiency of drug-resistant parasites. Int J Antimicrob Agents 2007; 29: 637-642
    CrossrefPubMedGoogle Scholar
  • 15 Khare CP. Indian herbal remedies: rational Western therapy, Ayurvedic, and other traditional usage, botany. Berlin: Springer; 2004
    CrossrefGoogle Scholar
  • 16 Tóro RM, Gessner AAF, Ceccarelli PS, Furtado NAJC, Albuquerque S, Bastos JK. Activity of the Pinus elliottii resin compounds against Lernaea cyprinacea in vitro . Vet Parasitol 2003; 118: 143-149
    CrossrefPubMedGoogle Scholar
  • 17 Yadav AK, Tangpu V. Therapeutic efficacy of Zanthoxylum rhetsa DC extract against experimental Hymenolepis diminuta (Cestoda) infections in rats. J Parasitol Dis 2009; 33: 42-47
    PubMedGoogle Scholar
  • 18 Chen W, Vermaak I, Viljoen A. Camphor – a fumigant during the Black Death and a coveted fragrant wood in ancient Egypt and Babylon – a review. Molecules 2013; 18: 5434-5454
    CrossrefPubMedGoogle Scholar
  • 19 Antony JP, Fyfe L, Smith H. Plant active components – a resource for antiparasitic agents?. Trends Parasitol 2005; 21: 462-468
    CrossrefPubMedGoogle Scholar
  • 20 Melo NI, Magalhães LG, Carvalho CE, Wakabayashi KAL, Aguiar GP, Ramos RC, Mantovani ALL, Turatti ICC, Rodrigues V, Groppo M, Cunha WR, Veneziani RCS, Crotti AEM. Schistosomicidal activity of the essential oil of Ageratum conyzoides L. (Asteraceae) against Schistosoma mansoni adult worms. Molecules 2011; 16: 762-773
    CrossrefPubMedGoogle Scholar
  • 21 Ambrósio SR, Arakawa NS, Esperandim VR, de Albuquerque S, Da Costa FB. Trypanocidal activity of pimarane diterpenes from Viguiera arenaria (Asteraceae). Phytother Res 2008; 22: 1413-1415
    CrossrefPubMedGoogle Scholar
  • 22 Muelas-Serrano S, Nogal-Ruiz JJ, Gómez-Barrio A. Setting of a colorimetric method to determine the viability of Trypanosoma cruzi epimastigotes. Parasitol Res 2000; 86: 999-1002
    CrossrefPubMedGoogle Scholar