Download PDF
Eur J Pediatr Surg 2014; 24(05): 403-409
DOI: 10.1055/s-0033-1352525
Original Article
Georg Thieme Verlag KG Stuttgart · New York

Protective Effect of Dimethyl Sulfoxide on Stricture Formation in Corrosive Esophageal Burns in Rats

Huseyin Kilincaslan
1   Department of Pediatric Surgery, Bezmialem Vakif University, Faculty of Medicine, Istanbul, Turkey
,
Hande Ozgun Karatepe
2   Department of Pediatrics, Zeynep Kamil Research and Education Hospital, Istanbul, Turkey
,
Fatma Sarac
3   Department of Pediatric Surgery, Haseki Research and Education Hospital, Istanbul, Turkey
,
Vakur Olgac
4   Department of Pathology, Istanbul University, Institute of Oncology, Istanbul, Turkey
,
Ahu Sarbay Kemik
5   Department of Biochemistry, Istanbul University, Cerrahpasa Faculty of Medicine, Istanbul, Turkey
,
Ahmet Hakan Gedik
6   Department of Pediatrics, Bezmialem Vakif University, Faculty of Medicine, Istanbul, Turkey
,
Omer Uysal
7   Department of Biostatistics, Bezmialem Vakif University, Faculty of Medicine, Istanbul, Turkey
› Author Affiliations
Further Information

Publication History

15 March 2013

26 June 2013

Publication Date:
05 August 2013 (online)

    Permissions and Reprints

Abstract

Introduction The aim of this study was to investigate the effects of dimethyl sulfoxide (DMSO) on stricture formation in corrosive esophageal burns.

Materials and Methods A total of 21 male rats were divided equally into three groups. In Group 1 (burn) and Group 2 (burn + DMSO) burns were induced in the distal esophagi with a 30% NaOH solution. In Group 3 (control), a saline solution was applied to the esophageal lumen. In Group 2, DMSO was administered intraperitoneally (3 mg/kg) 15 minutes after the burn was induced and then every 24 hours for 7 days. All rats were humanely killed at the end of Day 22. Distal esophagi were harvested for analysis. The stenosis index (SI) and histopathologic damage score were evaluated in addition to malondialdehyde (MDA), myeloperoxidase (MPO), nitric oxide (NO), tumor necrosis factor alpha (TNF-α), and interleukin-6 (IL-6) levels.

Results DMSO significantly decreased the levels of MDA, NO, TNF-α, and IL-6 in the rats with burned esophagi. Furthermore, the SI and histopathologic scores decreased significantly in the burn + DMSO group relative to the burn group (p < 0.05).

Conclusions Our results suggest that DMSO can decrease the occurrence of stricture formation and could represent a beneficial alternative therapy for the treatment of corrosive esophagitis.

Keywords

corrosive esophageal burn - dimethyl sulfoxide - esophageal stricture - oxidative stress
 

  • References

  • 1 Bingöl-Kologlu M, Tanyel FC, Müftüoğlu S , et al. The preventive effect of heparin on stricture formation after caustic esophageal burns. J Pediatr Surg 1999; 34 (2) 291-294
    CrossrefPubMedGoogle Scholar
  • 2 Günel E, Cağlayan F, Cağlayan O, Canbilen A, Tosun M. Effect of antioxidant therapy on collagen synthesis in corrosive esophageal burns. Pediatr Surg Int 2002; 18 (1) 24-27
    CrossrefPubMedGoogle Scholar
  • 3 Riffat F, Cheng A. Pediatric caustic ingestion: 50 consecutive cases and a review of the literature. Dis Esophagus 2009; 22 (1) 89-94
    CrossrefPubMedGoogle Scholar
  • 4 Kay M, Wyllie R. Caustic ingestions in children. Curr Opin Pediatr 2009; 21 (5) 651-654
    CrossrefPubMedGoogle Scholar
  • 5 Elshabrawi M, A-Kader HH. Caustic ingestion in children. Expert Rev Gastroenterol Hepatol 2011; 5 (5) 637-645
    CrossrefPubMedGoogle Scholar
  • 6 Contini S, Swarray-Deen A, Scarpignato C. Oesophageal corrosive injuries in children: a forgotten social and health challenge in developing countries. Bull World Health Organ 2009; 87 (12) 950-954
    CrossrefPubMedGoogle Scholar
  • 7 Corduk N, Koltuksuz U, Calli-Demirkan N, Rota S, Abban G, Sarioglu-Buke A. Effects of retinoic acid and zinc on the treatment of caustic esophageal burns. Pediatr Surg Int 2010; 26 (6) 619-624
    CrossrefPubMedGoogle Scholar
  • 8 Essani NA, Fisher MA, Jaeschke H. Inhibition of NF-kappa B activation by dimethyl sulfoxide correlates with suppression of TNF-alpha formation, reduced ICAM-1 gene transcription, and protection against endotoxin-induced liver injury. Shock 1997; 7 (2) 90-96
    CrossrefPubMedGoogle Scholar
  • 9 Kloesch B, Liszt M, Broell J, Steiner G. Dimethyl sulphoxide and dimethyl sulphone are potent inhibitors of IL-6 and IL-8 expression in the human chondrocyte cell line C-28/I2. Life Sci 2011; 89 (13-14) 473-478
    CrossrefPubMedGoogle Scholar
  • 10 Santos NC, Figueira-Coelho J, Martins-Silva J, Saldanha C. Multidisciplinary utilization of dimethyl sulfoxide: pharmacological, cellular, and molecular aspects. Biochem Pharmacol 2003; 65 (7) 1035-1041
    CrossrefPubMedGoogle Scholar
  • 11 Bini R, Olivero G, Trombetta A, Castagna E, Cotogni P. Effects of dimethyl sulfoxide, pyrrolidine dithiocarbamate, and methylprednisolone on nuclear factor-kappaB and heat shock protein 70 in a rat model of hemorrhagic shock. J Trauma 2008; 64 (4) 1048-1054
    CrossrefPubMedGoogle Scholar
  • 12 Kilincaslan H, Ozbey H, Olgac V. The effects of dimethyl sulfoxide on the acute phase of experimental acid and alkali corrosive esophageal burns. Eur Rev Med Pharmacol Sci 2013; (Ahead of print ID: ERMPS-4404).
    PubMedGoogle Scholar
  • 13 Koksal C, Bozkurt AK, Cangel U , et al. Attenuation of ischemia/reperfusion injury by N-acetylcysteine in a rat hind limb model. J Surg Res 2003; 111 (2) 236-239
    CrossrefPubMedGoogle Scholar
  • 14 Ergun Y, Koc A, Dolapcioglu K , et al. The protective effect of erythropoietin and dimethylsulfoxide on ischemia-reperfusion injury in rat ovary. Eur J Obstet Gynecol Reprod Biol 2010; 152 (2) 186-190
    CrossrefPubMedGoogle Scholar
  • 15 Guimarães SB, Kimura O, Vasconcelos PR. Dimethylsulfoxide attenuates ischemia-reperfusion injury in rat testis. Acta Cir Bras 2010; 25 (4) 357-361
    CrossrefPubMedGoogle Scholar
  • 16 Gehanno P, Guedon C. Inhibition of experimental esophageal lye strictures by penicillamine. Arch Otolaryngol 1981; 107 (3) 145-147
    CrossrefPubMedGoogle Scholar
  • 17 Liu AJ, Richardson MA. Effects of N-acetylcysteine on experimentally induced esophageal lye injury. Ann Otol Rhinol Laryngol 1985; 94 (5 Pt 1) 477-482
    PubMedGoogle Scholar
  • 18 Berthet B, di Costanzo J, Arnaud C, Choux R, Assadourian R. Influence of epidermal growth factor and interferon gamma on healing of oesophageal corrosive burns in the rat. Br J Surg 1994; 81 (3) 395-398
    CrossrefPubMedGoogle Scholar
  • 19 Kruidenier L, Kuiper I, Van Duijn W , et al. Imbalanced secondary mucosal antioxidant response in inflammatory bowel disease. J Pathol 2003; 201 (1) 17-27
    CrossrefPubMedGoogle Scholar
  • 20 Moshage H. Simple and reliable measurement of nitric oxide metabolites in plasma. Clin Chem 2009; 55 (10) 1881-1882
    CrossrefPubMedGoogle Scholar
  • 21 Yukselen V, Karaoglu AO, Ozutemiz O, Yenisey C, Tuncyurek M. Ketotifen ameliorates development of fibrosis in alkali burns of the esophagus. Pediatr Surg Int 2004; 20 (6) 429-433
    PubMedGoogle Scholar
  • 22 Ku SK, Seo BI, Park JH , et al. Effect of Lonicerae Flos extracts on reflux esophagitis with antioxidant activity. World J Gastroenterol 2009; 15 (38) 4799-4805
    CrossrefPubMedGoogle Scholar
  • 23 Günel E, Cağlayan F, Cağlayan O, Akillioğlu I. Reactive oxygen radical levels in caustic esophageal burns. J Pediatr Surg 1999; 34 (3) 405-407
    CrossrefPubMedGoogle Scholar
  • 24 Ocakci A, Coskun O, Tumkaya L , et al. Beneficial effects of Ebselen on corrosive esophageal burns of rats. Int J Pediatr Otorhinolaryngol 2006; 70 (1) 45-52
    CrossrefPubMedGoogle Scholar
  • 25 Bakan V, Garipardic M, Okumuş M , et al. The protective effect of erythropoietin on the acute phase of corrosive esophageal burns in a rat model. Pediatr Surg Int 2010; 26 (2) 195-201
    CrossrefPubMedGoogle Scholar
  • 26 Guven A, Gundogdu G, Sadir S , et al. The efficacy of ozone therapy in experimental caustic esophageal burn. J Pediatr Surg 2008; 43 (9) 1679-1684
    CrossrefPubMedGoogle Scholar
  • 27 Ceylan H, Yapici S, Tutar E, Ceylan NO, Tarakçıoğlu M, Demiryurek AT. Protective effects of dexpanthenol and y-27632 on stricture formation in a rat model of caustic esophageal injury. J Surg Res 2011; 171 (2) 517-523
    CrossrefPubMedGoogle Scholar
  • 28 Pascual C, Karzai W, Meier-Hellmann A , et al. Total plasma antioxidant capacity is not always decreased in sepsis. Crit Care Med 1998; 26 (4) 705-709
    CrossrefPubMedGoogle Scholar
  • 29 Cuzzocrea S, Riley DP, Caputi AP, Salvemini D. Antioxidant therapy: a new pharmacological approach in shock, inflammation, and ischemia/reperfusion injury. Pharmacol Rev 2001; 53 (1) 135-159
    PubMedGoogle Scholar
  • 30 Groeneveld AB, Sipkema P. Interaction of oxyradicals, antioxidants, and nitric oxide during sepsis. Crit Care Med 2000; 28 (6) 2161-2162
    CrossrefPubMedGoogle Scholar