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Drug Res (Stuttg) 2015; 65(05): 238-243
DOI: 10.1055/s-0034-1370966
Original Article
© Georg Thieme Verlag KG Stuttgart · New York

Effects of Teratogenic Drugs on CYP1A1 Activity in Differentiating Rat Embryo Cells

Gh. S. Tayeboon
1   Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
2   Department of Biology, Payame noor University, Tehran, Iran
,
S. N. Ostad   *
1   Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
3   Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran
,
S. Nasri
2   Department of Biology, Payame noor University, Tehran, Iran
,
A. Nili-Ahmadabadi
4   Department of Toxicology and Pharmacology, Faculty of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran
,
F. Tavakoli
1   Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
,
O. Sabzevari   *
1   Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
5   Toxicology and Poisoning Research Centre, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
› Author Affiliations
Further Information

Publication History

received 22 January 2014

accepted 16 February 2014

Publication Date:
25 March 2014 (online)

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Abstract

CYP1A1, a P450 isoenzyme, is involved in the phase I xenobiotic metabolism including teratogen drugs. In the present study, the ability of teratogens to elevate the embryonic expression of CYP1A1 was examined. Micromass cell cultures prepared from day 13 rat embryo limb buds (LB). LB cells were cultivated and exposed for 5 days to retinoic acid (RA), hydrocortisone (HC), caffeine (CA) and quinine (QN). CYP1A1 protein expression and activity were measured using immunofluorescence staining and ethoxyresorufin O-deethylation (EROD) assay, respectively. The EROD activity increased significantly following LB cells exposure to RA and HC (p<0.05) but the expression of CYP1A1 protein was reduced by these drugs, whereas the expression of CYP1A1 protein and EROD activity decreased significantly following the addition of CA and QN (p<0.05, p<0.01). Our findings show that studied teratogens have potency to increase CYP1A1 activity.

Key words

hydrocortisone - limb bud - micromass culture - retinoic acid

* Correspondence to Prof. Seyed Nasser Ostad for teratogenicity micromass culture assay and immunofluorescence microscopy & to Prof. Omid Sabzevari for enzyme activity assay.


 

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