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Planta Med 2014; 80(15): 1340-1344
DOI: 10.1055/s-0034-1383047
Biological Screening
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

In Vitro Antiprotozoal and Cytotoxic Activity of Ethnopharmacologically Selected Guinean Plants

Mohammed Sahar Traore
1   Department of Pharmacy, University Gamal Abdel Nasser of Conakry, Guinea
2   Research and Valorization Center on Medicinal Plants, Dubreka, Guinea
3   Laboratoire Pharmaceutique AMB-Pharma, Guinea
,
Sere Diane
1   Department of Pharmacy, University Gamal Abdel Nasser of Conakry, Guinea
2   Research and Valorization Center on Medicinal Plants, Dubreka, Guinea
,
Mamadou Saliou Telly Diallo
1   Department of Pharmacy, University Gamal Abdel Nasser of Conakry, Guinea
2   Research and Valorization Center on Medicinal Plants, Dubreka, Guinea
,
Elhadj Saïdou Balde
1   Department of Pharmacy, University Gamal Abdel Nasser of Conakry, Guinea
2   Research and Valorization Center on Medicinal Plants, Dubreka, Guinea
,
Mamadou Aliou Balde
1   Department of Pharmacy, University Gamal Abdel Nasser of Conakry, Guinea
2   Research and Valorization Center on Medicinal Plants, Dubreka, Guinea
3   Laboratoire Pharmaceutique AMB-Pharma, Guinea
,
Aïssata Camara
1   Department of Pharmacy, University Gamal Abdel Nasser of Conakry, Guinea
2   Research and Valorization Center on Medicinal Plants, Dubreka, Guinea
3   Laboratoire Pharmaceutique AMB-Pharma, Guinea
,
Abdoulaye Diallo
1   Department of Pharmacy, University Gamal Abdel Nasser of Conakry, Guinea
,
Abdoulaye Keita
1   Department of Pharmacy, University Gamal Abdel Nasser of Conakry, Guinea
2   Research and Valorization Center on Medicinal Plants, Dubreka, Guinea
,
Paul Cos
4   Laboratory of Microbiology, Parasitology and Hygiene (LMPH), University of Antwerp, Antwerp, Belgium
,
Louis Maes
4   Laboratory of Microbiology, Parasitology and Hygiene (LMPH), University of Antwerp, Antwerp, Belgium
,
Luc Pieters
5   Natural Products & Food Research and Analysis (NatuRA), Department of Pharmaceutical Sciences, University of Antwerp, Antwerp, Belgium
,
Aliou Mamadou Balde
1   Department of Pharmacy, University Gamal Abdel Nasser of Conakry, Guinea
2   Research and Valorization Center on Medicinal Plants, Dubreka, Guinea
3   Laboratoire Pharmaceutique AMB-Pharma, Guinea
› Author Affiliations
Further Information

Publication History

received 06 April 2014
revised 08 July 2014

accepted 04 August 2014

Publication Date:
02 September 2014 (online)

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Abstract

Based on an ethnobotanical survey, 41 Guinean plant species widely used in the traditional treatment of fever and/or malaria were collected. From these, 74 polar and apolar extracts were prepared and tested for their in vitro antiprotozoal activity along with their cytotoxicity on MRC-5 cells. A potent activity (IC50 < 5 µg/mL) was observed for Terminalia albida, Vismia guineensis, Spondias mombin, and Pavetta crassipes against Plasmodium falciparum; for Pavetta crassipes, Vismia guineensis, Guiera senegalensis, Spondias mombin, Terminalia macroptera, and Combretum glutinosum against Trypanosoma brucei brucei; for Bridelia ferruginea, G. senegalensis, V. guineensis, P. crassipes, and C. glutinosum against Trypanosoma cruzi. Only the extract of Tetracera alnifolia showed a good activity (IC50 8.1 µg/mL) against Leishmania infantum. The selectivity index of the active samples varied from 0.08 to > 100. These results may validate at least in part the traditional use of some of the plant species.

Key words

Guinea - antiprotozoal activity - Plasmodium - Trypanosoma - Leishmania - cytotoxicity
 

  • References

  • 1 Bero J, Quetin-Leclercq J. Natural products and tropical diseases. Planta Med 2011; 77: 571
    Article in Thieme ConnectPubMedGoogle Scholar
  • 2 WHO. World malaria report 2012. Geneva: WHO; 2012
    Google Scholar
  • 3 WHO. WHO traditional medicine strategy 2014 – 2023. Geneva: WHO; 2013
    Google Scholar
  • 4 Traore MS, Baldé MA, Diallo MST, Baldé ES, Diané S, Camara A, Diallo A, Baldé A, Kéita A, Kéita SM, Oularé K, Magassouba FB, Diakité I, Diallo A, Pieters L, Baldé AM. Ethnobotanical survey on medicinal plants used by Guinean traditional healers in the treatment of malaria. J Ethnopharmacol 2013; 150: 1145-1153
    CrossrefPubMedGoogle Scholar
  • 5 Jansen O, Angenot L, Tits M, Nicolas JP, De Mol P, Nikiéma J, Frédérich M. Evaluation of 13 selected medicinal plants from Burkina Faso for their antiplasmodial properties. J Ethnopharmacol 2010; 130: 143-150
    CrossrefPubMedGoogle Scholar
  • 6 Sanon S, Ollivier E, Azasc N, Mahioub V, Gasquet M, Ouattara CT, Nebied I, Traore AS, Esposito F, Balansard G, Timon-David P, Fumoux F. Ethnobotanical survey and in vitro antiplasmodial activity of plants used in traditional medicine in Burkina Faso. J Ethnopharmacol 2003; 86: 143-147
    CrossrefPubMedGoogle Scholar
  • 7 Mustofa. Valentin A, Benoit-Vical F, Pelissier Y, Kone-Bamba D, Mallie M. Antiplasmodial activity of plant extracts used in West-African traditional medicine. J Ethnopharmacol 2000; 73: 145-151
    CrossrefPubMedGoogle Scholar
  • 8 Mariath IR, de S Falcão H, Barbosa-Filho JM, de Sousa LCF, de A Tomaz AC, Batista LM, de Fátima FM, Diniz M, Athayde-Filho PF, Tavares JF, Silva MS, da Cunha EVL. Plants of the American continent with antimalarial activity. Brazilian J Pharmacogn 2009; 19: 158-192
    PubMedGoogle Scholar
  • 9 Hoet S, Opperdoes F, Brun R, Adjakidje V, Quetin-Leclercq J. In vitro antitrypanosomal activity of ethnopharmacologically selected Beninese plants. J Ethnopharmacol 2004; 91: 37-42
    CrossrefPubMedGoogle Scholar
  • 10 Zofou D, Tene M, Ngemenya MN, Tane P, Titanji VPK. In vitro antiplasmodial activity and cytotoxicity of extracts of selected medicinal plants used by traditional healers of Western Cameroon. Malar Res Treat advance online publication 12.04.2011; DOI: 10.4061/2011/561342.
    PubMedGoogle Scholar
  • 11 Weenen H, Nkunya MHH, Bray DH, Mwasumbi LB, Kinabo LS. Antimalarial activity of Tanzanian medicinal plants. Planta Med 1990; 56: 368-370
    Article in Thieme ConnectPubMedGoogle Scholar
  • 12 Ruiz L, Maco M, Cobos M, Choquevilca ALG, Roumy V. Plants used by native Amazonian groups from the Nanay River (Peru) for the treatment of malaria. J Ethnopharmacol 2011; 133: 917-921
    CrossrefPubMedGoogle Scholar
  • 13 Zederkopff Ballin N, Traore M, Tinto H, Sittie A, Molgaard P, Olsen CE, Kharazmi A, Christensen SB. Antiplasmodial compounds from Cochlospermum tinctorium . J Nat Prod 2002; 65: 1325-1327
    CrossrefPubMedGoogle Scholar
  • 14 Ancolio C, Azas N, Mahiou V, Ollivier F, Di Giorgio C, Keita A, Timon-David P, Balansard G. Antimalarial activity of extracts and alkaloids isolated from six plants used in traditional medicine in Mali and São-Tomé. Phytotherapy Res 2002; 16: 646-649
    CrossrefPubMedGoogle Scholar
  • 15 Kamanzi Atindehou K, Schmid C, Brun R, Koné MW, Traoré D. Antitrypanosomal and antiplasmodial activity of medicinal plants from Côte dʼIvoire. J Ethnopharmacol 2004; 90: 221-227
    CrossrefPubMedGoogle Scholar
  • 16 Soh PN, Benoit-Vical F. Are West African plants a source of future antimalarial drugs?. J Ethnopharmacol 2007; 114: 130-140
    CrossrefPubMedGoogle Scholar
  • 17 Willcox M, Chamberlain J. Neem (Azadirachta indica). In: Willcox M, Bodeker G, Rasoanaivo P, editors Traditional herbal medicines for modern times. Boca Raton: CRC Press; 2004: 91-115
    Google Scholar
  • 18 Shuaibu MN, Wuyep PA, Yanagi T, Hirayama K, Tanaka T, Kouno I. The use of microfluorometric method for activity-guided isolation of antiplasmodial compound from plant extracts. Parasitol Res 2008; 102: 1119-1127
    CrossrefPubMedGoogle Scholar
  • 19 Muganga R, Angenot L, Tits M, Frédérich M. Antiplasmodial and cytotoxic activities of Rwandan medicinal plants used in the treatment of malaria. J Ethnopharmacol 2010; 128: 52-57
    CrossrefPubMedGoogle Scholar
  • 20 Omulokoli E, Khan B, Chhabra SC. Antiplasmodial activity of four Kenyan medicinal plants. J Ethnopharmacol 1997; 56: 133-137
    CrossrefPubMedGoogle Scholar
  • 21 Saidu K, Onah J, Orisadipe A, Olusola A, Wambebe C, Gamaniel K. Antiplasmodial, analgesic and anti-inflammatory activities of the aqueous extract of the stem bark of Erythrina senegalensis . J Ethnopharmacol 2000; 71: 275-280
    CrossrefPubMedGoogle Scholar
  • 22 Adebayo JO, Krettli AU. Potential antimalarials from Nigerian plants: a review. J Ethnopharmacol 2000; 133: 289-302
    PubMedGoogle Scholar
  • 23 Banzoui JT, Prado R, Menan H, Valentin A, Roumestan C, Mallie M, Pelissier Y, Blache Y. In vitro antiplasmodial activity of extracts of Alchornea cordifolia and identification of an active constituent: ellagic acid. J Ethnopharmacol 2002; 81: 399-401
    CrossrefPubMedGoogle Scholar
  • 24 Soh PN, Witkowski B, Olagnier D, Nicolau ML, Garcia-Alvarez MC, Berry A, Benoit-Vical F. In vitro and in vivo properties of ellagic acid in malaria treatment. Antimicrob Agents Chemother 2009; 53: 1100-1106
    CrossrefPubMedGoogle Scholar
  • 25 Dhooghe L, Meert H, Cimanga RK, Vlietinck AJ, Pieters L, Apers S. The quantification of ellagic acid in the crude extract of Phyllanthus amarus Schum. & Thonn. (Euphorbiaceae). Phytochem Anal 2011; 22: 361-366
    CrossrefPubMedGoogle Scholar
  • 26 Francois G, Steenackers T, Assi LA, Steglich W, Lamottke K, Holenz J, Bringmann G. Vismione H and structurally related anthranoid compounds of natural and synthetic origin as promising drugs against the human malaria parasite Plasmodium falciparum: structure-activity relationships. Parasitol Res 1999; 85: 582-588
    CrossrefPubMedGoogle Scholar
  • 27 Mbwambo ZH, Apers S, Moshi MJ, Kapingu MC, Van Miert S, Claeys M, Brun R, Cos P, Pieters L, Vlietinck AJ. Anthranoid compounds with antiprotozoal activity from Vismia orientalis . Planta Med 2004; 70: 706-710
    Article in Thieme ConnectPubMedGoogle Scholar
  • 28 Akubue PL, Mittal GC, Aguwa CN. Preliminary pharmacological study of some Nigerian medicinal plants. J Ethnopharmacol 1983; 8: 53-63
    CrossrefPubMedGoogle Scholar
  • 29 Offiah VN, Anyanwu LL. Abortifacient activity of an aqueous extract of Spondias mombin leaves. J Ethnopharmacol 1983; 26: 317-320
    PubMedGoogle Scholar
  • 30 Cos P, Vlietinck AJ, Vanden Berghe D, Maes L. Anti-infective potential of natural products: how to develop a stronger in vitro proof-of-concept. J Ethnopharmacol 2006; 106: 290-302
    CrossrefPubMedGoogle Scholar
  • 31 Mesia GK, Tona GL, Nanga TH, Cimanga RK, Apers S, Cos P, Maes L, Pieters L, Vlietinck AJ. Antiprotozoal and cytotoxic screening of 45 plant extracts from Democratic Republic of Congo. J Ethnopharmacol 2008; 115: 409-415
    CrossrefPubMedGoogle Scholar