Dynamic Change of Myogenin in Denervated Rat Mimetic Muscle

Abstract

We previously reported double innervation of rat mimetic muscles with labeling of facial nuclei. However, whether denervated mimetic muscles are affected after such nerve repair is not known. Rats were divided into five groups: Group A, controls; Group B, complete facial palsy; Group C, complete facial palsy with repair using end-to-end neurorrhaphy; Group D, incomplete facial palsy; and Group E, incomplete facial palsy with repair using end-to-side neurorrhaphy. Preoperatively and postoperatively, facial palsy and myogenin (Myog) expression in mimetic muscles were evaluated. Expression peaked on day 7 in Group B but was lower in Groups C and D. Expression in Groups D and E was comparable on day 28, and each model's score showed characteristic changes. Myog expression in facial mimetic muscles increases with denervation and decreases with nerve repair. Determining Myog expression levels in mimetic muscles just after nerve repair may help surgeons predict postoperative prognosis in facial palsy.

Keywords

myogenin - facial palsy - reinnervation

Disclosure

This work was supported by a grant-in-aid for exploratory research from the Japanese Ministry of Education, Culture, Sports, Science and Technology (10001881).

• References

• 1 May M, Sobol SM, Mester SJ. Hypoglossal-facial nerve interpositional-jump graft for facial reanimation without tongue atrophy. Otolaryngol Head Neck Surg 1991; 104 (6) 818-825
• 2 Yoleri L, Songür E, Yoleri O, Vural T, Cağdaş A. Reanimation of early facial paralysis with hypoglossal/facial end-to-side neurorrhaphy: a new approach. J Reconstr Microsurg 2000; 16 (5) 347-355 , discussion 355–356
• 3 Yamamoto Y, Sekido M, Furukawa H, Oyama A, Tsutsumida A, Sasaki S. Surgical rehabilitation of reversible facial palsy: facial-hypoglossal network system based on neural signal augmentation/neural supercharge concept. J Plast Reconstr Aesthet Surg 2007; 60: 223-231
• 4 Furukawa H, Saito A, Mol W, Sekido M, Sasaki S, Yamamoto Y. Double innervation occurs in the facial mimetic muscles after facial-hypoglossal end-to-side neural repair: rat model for neural supercharge concept. J Plast Reconstr Aesthet Surg 2008; 61 (3) 257-264
• 5 Shichinohe R, Furukawa H, Sekido M, Saito A, Hayashi T, Funayama E. Direction of innervation after interpositional nerve graft between facial and hypoglossal nerves in individuals with or without facial palsy: a rat model for treating incomplete facial palsy. J Plast Reconstr Aesthet Surg 2012; 65 (6) 763-770
• 6 Edmondson DG, Olson EN. A gene with homology to the myc similarity region of MyoD1 is expressed during myogenesis and is sufficient to activate the muscle differentiation program. Genes Dev 1989; 3 (5) 628-640
• 7 Wright WE, Sassoon DA, Lin VK. Myogenin, a factor regulating myogenesis, has a domain homologous to MyoD. Cell 1989; 56 (4) 607-617
• 8 Buckingham ME. Muscle: the regulation of myogenesis. Curr Opin Genet Dev 1994; 4 (5) 745-751
• 9 Sabourin LA, Rudnicki MA. The molecular regulation of myogenesis. Clin Genet 2000; 57 (1) 16-25
• 10 Eftimie R, Brenner HR, Buonanno A. Myogenin and MyoD join a family of skeletal muscle genes regulated by electrical activity. Proc Natl Acad Sci U S A 1991; 88 (4) 1349-1353
• 11 Davis RL, Weintraub H, Lassar AB. Expression of a single transfected cDNA converts fibroblasts to myoblasts. Cell 1987; 51 (6) 987-1000
• 12 Braun T, Bober E, Buschhausen-Denker G, Kohtz S, Grzeschik KH, Arnold HH. Differential expression of myogenic determination genes in muscle cells: possible autoactivation by the Myf gene products. EMBO J 1989; 8 (12) 3617-3625
• 13 Rhodes SJ, Konieczny SF. Identification of MRF4: a new member of the muscle regulatory factor gene family. Genes Dev 1989; 3 (12B): 2050-2061
• 14 Miner JH, Wold B. Herculin, a fourth member of the MyoD family of myogenic regulatory genes. Proc Natl Acad Sci U S A 1990; 87 (3) 1089-1093
• 15 Donghui C, Shicai C, Wei W , et al. Functional modulation of satellite cells in long-term denervated human laryngeal muscle. Laryngoscope 2010; 120 (2) 353-358
• 16 Chen L, Huang HW, Gu SH, Xu L, Gu YD, Xu JG. The study of myogenin expression in denervated human skeletal muscles. J Int Med Res 2011; 39 (2) 378-387
• 17 Chen D, Chen S, Wang W, Liu F, Zhang C, Zheng H. Modulation of satellite cells in rat facial muscle following denervation and delayed reinnervation. Acta Otolaryngol 2010; 130 (12) 1411-1420
• 18 Macpherson PCD, Wang X, Goldman D. Myogenin regulates denervation-dependent muscle atrophy in mouse soleus muscle. J Cell Biochem 2011; 112 (8) 2149-2159
• 19 Teraoka M, Hato N, Takahashi H , et al. Myogenin expression in facial muscle following damage to the facial nerve. Acta Otolaryngol 2012; 132 (7) 783-787
• 20 Patodia S, Raivich G. Downstream effector molecules in successful peripheral nerve regeneration. Cell Tissue Res 2012; 349 (1) 15-26
• 21 Giger RJ, Hollis II ER, Tuszynski MH. Guidance molecules in axon regeneration. Cold Spring Harb Perspect Biol 2010; 2 (7) a001867
• 22 Ide C. Peripheral nerve regeneration. Neurosci Res 1996; 25 (2) 101-121