The Thrombogram: Monitoring Thrombin Generation in Platelet Rich Plasma

Summary

A method is described in which thrombin activity in clotting plasma can be monitored through the continuous measurement of the fluorescent split-product of the substrate Z-Gly-Gly-Arg-AMC. The signal is not impaired by turbidity; therefore proper measurement is not disturbed by the occurrence of a clot or the presence of platelets and direct measurement in platelet rich plasma is possible.

Keywords

Thrombin generation - thrombin potential - thrombogram - platelet rich plasma

• References

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