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Thromb Haemost 1998; 79(04): 784-789
DOI: 10.1055/s-0037-1615065
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Schattauer GmbH

Weight-Loss Induced Changes in Plasma Factor VII Coagulant Activity and Relation to the Factor VII Arg/Gln353 Polymorphism in Moderately Obese Adults

James S. Pankow
1   From the Department of Epidemiology, School of Public Health, University of North Carolina at Chapel Hill, Chapel Hill, NC
,
Aaron R. Folsom
2   From the Division of Epidemiology, School of Public Health, University of Minnesota, Minneapolis, MN
,
Eyal Shahar
2   From the Division of Epidemiology, School of Public Health, University of Minnesota, Minneapolis, MN
,
Michael Y. Tsai
3   From the Department of Laboratory Medicine and Pathology, University of Minnesota Medical School, Minneapolis, MN
,
Robert W. Jeffery
2   From the Division of Epidemiology, School of Public Health, University of Minnesota, Minneapolis, MN
,
Rena R. Wing
4   From the Western Psychiatric Institute and Clinic, University of Pittsburgh Medical Center, Pittsburgh, PA, USA
› Author AffiliationsThis work was supported by National Institutes of Health grants HL-41332 (R.W.J.) and HL-41330 (R.R.W.). Dr. Pankow was partially supported by an Institutional National Research Service Award (T32 HL-07036) from the National Heart, Lung, and Blood Institute.
Further Information

Publication History

Received 26 May 1997

Accepted after resubmission 10 December 1997

Publication Date:
07 December 2017 (online)

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Summary

Elevated plasma factor VII coagulant activity (factor VIIc) may be an independent risk factor for coronary heart disease. Several cross-sectional studies suggest that a polymorphism of the factor VII gene (ArgGln353) interacts with plasma triglyceride level in determining factor VIIc, but prospective data are lacking. Factor VII genotype, factor VIIc, and triglyceride level were measured in moderately obese adults aged 25 to 45 who underwent a six-month clinical trial to evaluate strategies for weight loss. A total of 48 men and 50 women who experienced substantial weight loss (mean: 10 kg) provided samples for genetic analysis. Overall, 78% of participants were homozygous for the Arg353 allele, while the remaining 22% were heterozygous (Arg/Gln353). At the baseline examination, heterozygotes had lower mean factor VIIc than Arg353 homozygotes (92% vs. 112%; p<0.001), and genotype explained 18% of the variance of factor VIIc. Average six-month weight loss was similar in both genotypes; mean reductions in factor VIIc following weight loss were greatest among Arg353 homozygotes with high initial values (>120%). Cross-sectional and longitudinal associations between plasma factor VIIc and triglyceride level were not dependent on genotype. These data confirm that the Gln353 allele is associated with lower factor VII coagulant activity in moderately obese adults, but they do not support the hypothesis that the Arg-Gln353 polymorphism interacts with plasma triglyceride level in determining factor VIIc.

 

  • References

  • 1 Morrissey JH. Tissue factor interactions with factor VII: measurement and clinical significance of factor VIIa in plasma. Blood Coagul Fibrinolysis 1995; 6 (Suppl. 01) S14-S9.
    CrossrefPubMedGoogle Scholar
  • 2 Meade TW. Haemostatic function, arterial disease and the prevention of arterial thrombosis. Ballieres Clin Haematol 1994; 7: 733-55.
    CrossrefPubMedGoogle Scholar
  • 3 Meade TW, Mellows S, Brozovic M, Miller GJ, Chakrabarti RR, North WRS, Haines AP, Stirling Y, Imeson JD, Thompson SG. Haemostatic function and ischaemic heart disease: principal results of the Northwick Park Heart Study. Lancet 1986; 2: 533-7.
    PubMedGoogle Scholar
  • 4 Assmann G, Cullen P, Heinrich J, Schulte H. Hemostatic variables in the prediction of coronary risk: results of the 8 year follow-up of healthy men in the Münster Heart Study (PROCAM). Isr J Med Sci 1996; 32: 364-70.
    PubMedGoogle Scholar
  • 5 Folsom AR, Wu KK, Rosamond WD, Sharett AR, Chambless LE. Prospective study of hemostatic factors and incidence of coronary heart disease. The Atherosclerosis Risk in Communities (ARIC) Study. Circulation 1997; 96: 1102-8.
    CrossrefPubMedGoogle Scholar
  • 6 Green F, Kelleher C, Wilkes H, Temple A, Meade T, Humphries S. A common genetic polymorphism associated with lower coagulation factor VII levels in healthy individuals. Arterioscler Thromb 1991; 11: 540-6.
    CrossrefPubMedGoogle Scholar
  • 7 Lane A, Cruickshank JK, Mitchell J, Henderson A, Humphries S, Green F. Genetic and environmental determinants of factor VII coagulant activity in ethnic groups at differing risk of coronary heart disease. Atherosclerosis 1992; 94: 43-50.
    CrossrefPubMedGoogle Scholar
  • 8 Humphries SE, Lane A, Green FR, Cooper J, Miller GJ. Factor VII coagulant activity and antigen levels in healthy men are determined by interaction between factor VII genotype and plasma triglyceride concentration. Arterioscler Thromb 1994; 14: 193-8.
    CrossrefPubMedGoogle Scholar
  • 9 Koster T, Rosendaal FR, Reitsma PH, van der Velden PA, Briet E, Vandenbroucke JP. Factor VII and fibrinogen as risk factors for venous thrombosis. Thromb Haemost 1994; 71: 719-22.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 10 Saha N, Liu Y, Heng CK, Hong S, Low PS, Tay JSH. Association of factor VII genotype with plasma factor VII activity and antigen levels in healthy Indian adults and interaction with triglyceride. Arterioscler Thromb 1994; 14: 1923-7.
    CrossrefPubMedGoogle Scholar
  • 11 Kario K, Narita N, Matsuo T, Kayaba K, Tsutsumi A, Matsuo M, Miyata T, Shimada K. Genetic determinants of plasma factor VII activity in the Japanese. Thromb Haemost 1995; 73: 617-22.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 12 Silveira A, Green F, Karpe F, Blombäck M, Humphries S, Hamsten A. Elevated levels of factor VII activity in the postprandial state: effect of the factor VII Arg-Gln polymorphism. Thromb Haemost 1994; 72: 734-9.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 13 Meilahn E, Ferrell R, Kiss J, Temple A, Green F, Humphries S, Kuller L. Genetic determination of coagulation factor VIIc levels among healthy middle-aged women. Thromb Haemost 1995; 73: 623-5.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 14 Moor E, Silveira A, van’t Hooft F, Suontaka AM, Eriksson P, Blombäck M, Hamsten A. Coagulation factor VII mass and activity in young men with MI at a young age. Role of plasma lipoproteins and factor VII genotype. Arterioscler Thromb Vasc Biol 1995; 15: 655-64.
    CrossrefPubMedGoogle Scholar
  • 15 Bernardi F, Marchetti G, Pinotti M, Arcieri P, Baroncini C, Papacchini M, Zepponi E, Ursicino N, Chiarotti F, Mariani G. Factor VII gene polymorphisms contribute about one third of the factor VII level variation in plasma. Arterioscler Thromb Vasc Biol 1996; 16: 72-6.
    PubMedGoogle Scholar
  • 16 Lane A, Green F, Scarabin PY, Nicaud V, Bara L, Humphries S, Evans A, Luc G, Cambou JP, Arveiler D, Cambien F. Factor VII Arg/Gln353 polymorphism determines factor VII coagulant activity in patients with myocar-dial infarction (MI) and control subjects in Belfast and in France but is not a strong indicator of MI risk in the ECTIM study. Atherosclerosis 1996; 119: 119-27.
    CrossrefPubMedGoogle Scholar
  • 17 Humphries S, Temple A, Lane A, Green F, Cooper J, Miller G. Low plasma levels of factor VIIc and antigen are more strongly associated with the 10 base pair promoter (-323) insertion than the glutamine 353 variant. Thromb Haemost 1996; 75: 567-72.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 18 Mansfield MW, Heywood DM, Grant PJ. Circulating levels of factor VII, fibrinogen, and von Willebrand factor and features of insulin resistance in first-degree relatives of patients with NIDDM. Circulation 1996; 94: 2171-6.
    CrossrefPubMedGoogle Scholar
  • 19 Heywood DM, Mansfield MW, Grant PJ. Factor VII gene polymorphisms, factor VII:c levels and features of insulin resistance in non-insulin-dependent diabetes mellitus. Thromb Haemost 1996; 75: 401-6.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 20 Heywood DM, Carter AM, Catto AJ, Bamford JM, Grant PJ. Polymorphisms of the factor VII gene and circulating FVII:c levels in relation to acute cerebrovascular disease and poststroke mortality. Stroke 1997; 28: 816-21.
    CrossrefPubMedGoogle Scholar
  • 21 Wing RR, Jeffery RW, Burton LR, Thorson C, Kuller LH, Folsom AR. Change in waist-hip ratio with weight loss and its association with change in cardiovascular risk factors. Am J Clin Nutr 1992; 55: 1086-92.
    CrossrefPubMedGoogle Scholar
  • 22 Folsom AR, Qamhieh HT, Wing RR, Jeffery RW, Stinson VL, Kuller LH, Wu KK. Impact of weight loss on plasminogen activator inhibitor (PAI-1), factor VII, and other hemostatic factors in moderately overweight adults. Arterioscler Thromb 1993; 13: 162-9.
    CrossrefPubMedGoogle Scholar
  • 23 Paffenbarger Jr RS, Wing AL, Hyde RT. Physical activity as an index of heart attack risk in college alumni. Am J Epidemiol 1978; 108: 161-75.
    CrossrefPubMedGoogle Scholar
  • 24 Allain CC, Poon LS, Chan CSG, Richmond W, Fu PC. Enzymatic determination of total serum cholesterol. Clin Chem 1974; 20: 470-5.
    PubMedGoogle Scholar
  • 25 Bucolo G, David H. Quantitative determination of serum triglycerides by the use of enzymes. Clin Chem 1973; 19: 476-82.
    PubMedGoogle Scholar
  • 26 Warnick GR, Albers JJ. A comprehensive evaluation of the heparinmanganese precipitation procedure for estimating high density lipoprotein cholesterol. J Lipid Res 1978; 19: 65-76.
    CrossrefPubMedGoogle Scholar
  • 27 Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without the use of the preparative ultracentrifuge. Clin Chem 1972; 18: 499-502.
    PubMedGoogle Scholar
  • 28 Herbert V, Lau KS, Gottlieb CW. Coated charcoal immunoassay of insulin. J Clin Endocrinol 1965; 25: 1375-4.
    CrossrefPubMedGoogle Scholar
  • 29 Newton CR, Graham A, Heptinstall LE, Powell SJ, Summers C, Kalsheker N, Smith JC, Markham AF. Analysis of any point mutation in DNA. The amplification refractory mutation system (ARMS). Nucleic Acids Res 1989; 17: 2503-16.
    CrossrefPubMedGoogle Scholar
  • 30 Kleinbaum DG, Kupper LL, Muller KE. Applied regression analysis and other multivariable methods. 2nd ed. PWS-Kent; Boston, MA: 1988. pp 271-2.
    Google Scholar
  • 31 Despres JP, Moorjani S, Lupien PJ, Tremblay A, Nadeau A, Bouchard C. Genetic aspects of susceptibility to obesity and related dyslipidemias. Mol Cell Biochem 1992; 113: 151-69.
    PubMedGoogle Scholar
  • 32 Silveira A, Karpe F, Johnsson H, Bauer KA, Hamsten A. In vivo demonstration in humans that large postprandial triglyceride-rich lipoproteins activate coagulation factor VII through the intrinsic coagulation pathway. Arterioscler Thromb Vasc Biol 1996; 16: 1333-9.
    CrossrefPubMedGoogle Scholar
  • 33 Miller GJ. Lipoproteins and the haemostatic system in atherothrombotic disorders. Ballieres Clin Haematol 1994; 7: 713-1.
    CrossrefPubMedGoogle Scholar
  • 34 Pollak ES, Hung H-L, Godin W, Overton GC, High KA. Functional characterization of the human factor VII 5’ flanking region. J Biol Chem 1996; 271: 1738-47.
    CrossrefPubMedGoogle Scholar
  • 35 Sacchi E, Tagliabue L, Scoglio R, Baroncini C, Coppola R, Bernardi F, Mannucci PM. Plasma factor VII levels are influenced by a polymorphism in the promoter region of the FVII gene. Blood Coag Fibrinol 1996; 7: 114-7.
    CrossrefPubMedGoogle Scholar
  • 36 Niskanen L, Schwab US, Sarkkinen ES, Krusius T, Vahtera E, Uusitupa MIJ. Effects of dietary fat modification on fibrinogen, factor VII, and plasminogen activator inhibitor-1 activity in subjects with impaired glucose tolerance. Metabolism 1997; 46: 666-72.
    CrossrefPubMedGoogle Scholar
  • 37 Miller GJ, Stirling Y, Esnouf MP, Heinrich J, van de Loo J, Kienast J, Wu KK, Morrissey JH, Meade TW, Martin JC, Imeson JD, Cooper JA, Finch A. Factor VII-deficient substrate plasmas depleted of protein C raise the sensitivity of the factor VII bio-assay to activated factor VII: an international study. Thromb Haemost 1994; 71: 38-48.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 38 Kario K, Matsuo T, Asada R, Sakata T, Kato H, Miyata T. The strong positive correlation between factor VII clotting activity using bovine thromboplastin and the activated factor VII level. Thromb Haemost 1995; 73: 429-34.
    Article in Thieme ConnectPubMedGoogle Scholar