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Thromb Haemost 1998; 79(05): 1004-1007
DOI: 10.1055/s-0037-1615110
Review Article
Schattauer GmbH

Detection of Platelets Activated during Acetylcholine-Induced Coronary Vasospasm

Teruo Inoue
1   From the Department of Cardiology, Koshigaya Hospital, Dokkyo University School of Medicine, Saitama, Japan
,
Tsuneo Fujito
1   From the Department of Cardiology, Koshigaya Hospital, Dokkyo University School of Medicine, Saitama, Japan
,
Kazuhiro Hoshi
1   From the Department of Cardiology, Koshigaya Hospital, Dokkyo University School of Medicine, Saitama, Japan
,
Yoshihiko Sakai
1   From the Department of Cardiology, Koshigaya Hospital, Dokkyo University School of Medicine, Saitama, Japan
,
Shigenori Morooka
1   From the Department of Cardiology, Koshigaya Hospital, Dokkyo University School of Medicine, Saitama, Japan
,
Ryoichi Sohma
1   From the Department of Cardiology, Koshigaya Hospital, Dokkyo University School of Medicine, Saitama, Japan
› Author Affiliations
Further Information

Publication History

Received 19 July 1996

Accepted after revision 19 December 1997

Publication Date:
07 December 2017 (online)

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Summary

Although platelet activation may play a role in coronary artery spasm, platelets activated following coronary vasospasm have not been clinically detected. We performed flow cytometric analysis of activation-dependent granular proteins, CD62P (P-selectin), CD63, PAC-1 (activated glycoprotein [GP] IIb/IIIa) and thrombospondin on the platelet plasma membrane in patients who exhibited acetylcholine-induced coronary vasospasm and compared findings with those in control patients without vasospasm. We simultaneously investigated the plasma levels of thrombin anti-thrombin III complex (TAT), plasmin α2-plasmin inhibitor complex (PIC), and thrombomodulin. In patients with vasospasm, the expression of CD62P, CD63 and PAC-1 on the platelet membrane surface increased in coronary sinus blood samples following coronary vasospasm, although the expression in aortic samples did not change. The TAT level also increased in the coronary sinus after vasospasm. Platelets might be activated by coronary vasospasm within the coronary circulation. The platelet activation process may be modulated by thrombin generation.

 

  • References

  • 1 Cohen RA, Shepherd JT, Vanhoutte PM. Inhibitory role of the endothelium in the response of isolated coronary arteries to platelet. Science 1983; 221: 273-4.
    CrossrefPubMedGoogle Scholar
  • 2 Oshima S, Yasue H, Ogawa H, Okumura K, Matsuyama K. Fibrinopeptide A is released into the coronary circulation after coronary spasm. Circulation 1990; 82: 2222-5.
    CrossrefPubMedGoogle Scholar
  • 3 Stenberg PE, McEver RP, Shuman MA, Jaques YV, Bainton DF. A platelet alpha-granule membrane protein (GMP-140) is expressed on the plasma membrane after activation. J Cell Biol 1985; 101: 880-6.
    CrossrefPubMedGoogle Scholar
  • 4 Nieuwenhuis HK, van Oosterhout JJG, Rozenmuller E, van Iwaarden F, Sixma JJ. Studies with a monoclonal antibody against activated platelet: evidence that a secreted 53,000-molecular weight lysosome-like granule protein is exposed on the surface of activated platelet in the circulation. Blood 1987; 70: 838-45.
    PubMedGoogle Scholar
  • 5 Shattil SJ, Hoxie JA, Cunningham MC, Brass LF. Changes in the platelet membrane glycoprotein IIb-IIIa complex during platelet activation. J Biol Chem 1985; 260: 11107-14.
    PubMedGoogle Scholar
  • 6 Silverstein R, Asch AS, Nachmann RL. Glycoprotein IV mediates thrombospondin-dependent platelet-monocyte and platelet-U937 cell adhesion. J Clin Invest 1989; 84: 546-52.
    CrossrefPubMedGoogle Scholar
  • 7 Shattil SJ, Cunningham M, Hoxie JA. Detection of activated platelets in whole blood using activated-dependent monoclonal antibodies and flow cytometry. Blood 1987; 70: 307-15.
    PubMedGoogle Scholar
  • 8 Inoue T, Hoshi K, Fujito T, Sakai Y, Morooka S, Sohma R. Early detection of platelet activation after coronary angioplasty. Coronary Artery Dis 1996; 7: 529-34.
    CrossrefPubMedGoogle Scholar
  • 9 Pelzer H, Schwarz A, Heimburger N. Determination of human thrombin-anti thrombin III complex in plasma with an enzyme-linked immunosorbent assay. Thromb Haemost 1988; 59: 101-6.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 10 Holvoet P, de Boer A, Verstreken M, Collen D. An enzyme-linked immunosorbent assay (ELISA) for the measurement of plasmin-α2-antiplasmin complex in human plasma: application to the detection of in vivo activation of the fibrinolytic system. Thromb Haemost 1986; 56: 124-7.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 11 Esmon CT, Owen WG. Identification of an endothelial cell cofactor for thrombin-catalyzed activation of protein C. Proc Natl Acad Sci USA 1981; 78: 2249-52.
    CrossrefPubMedGoogle Scholar
  • 12 Hoshi K, Inoue T, Kakoi H, Satoh T, Yamaguchi H, Sakai Y, Morooka S, Takabatake Y. Plasma thrombomodulin levels in coronary sinus blood in patients with coronary artery disease. Blood Coagl Fibrinolysis 1995; 6: 129-32.
    PubMedGoogle Scholar
  • 13 Yasue H, Horio Y, Nakamura N, Fujii H, Imoto N, Sonoda R. et al. Induction of coronary artery spasm by acetylcholine in patients with variant angina: possible role of the parasympathetic nervous system in the pathogenesis of coronary artery spasm. Circulation 1986; 74: 955-63.
    CrossrefPubMedGoogle Scholar
  • 14 Bonfanti R, Furie BC, Furie B, Wagner DD. PADGEM (GMP-140) is a component of Weibel-Palade bodies of human endothelial cells. Blood 1989; 73: 1109-12.
    PubMedGoogle Scholar
  • 15 Merhi Y, Guidoin R, Provost P, Leung TK, Lam JYT. Increase of neutrophil adhesion and vasoconstriction with platelet deposition after deep arterial injury by angioplasty. Am Heart J 1995; 129: 445-51.
    CrossrefPubMedGoogle Scholar
  • 16 Matsuno K. Detection of platelet activation. Jpn J Clin Pathol 1995; 43: 432-7.
    PubMedGoogle Scholar
  • 17 Ruggeri ZM. Receptor-specific antiplatelet therapy. Circulation 1989; 1920-2.
    PubMedGoogle Scholar
  • 18 Spangenberg P, Redlich H, Bergmann I, Losche W, Gotzrath M, Kehrel B. The platelet glycoprotein IIb/IIIa complex is involved in the adhesion of activated platelets to leukocytes. Thromb Haemost 1993; 70: 514-21.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 19 Gold HK, Gimple LW, Yasuda T, Leinbach RC, Werner W, Holt R, Jordan R, Berger H, Collen D, Coller BS. Pharmacodynamic study of F(ab’)2 fragments of murine monoclonal antibody 7E3 directed against human platelet glycoprotein IIb/IIIa in patients with unstable angina pectoris. J Clin Invest 1990; 86: 651-9.
    CrossrefPubMedGoogle Scholar
  • 20 Scharf RE, Tomer A, Marzec UM, Teirstein PS, Ruggeri ZM, Harker LA. Activation of platelets in blood perfusing angioplasty-damaged coronary arteries: flow cytometric detection. Arteriosclerosis Thromb 1992; 12: 1475-87.
    CrossrefPubMedGoogle Scholar
  • 21 Grande P, Grauholt AM, Madsen JK. Unstable angina pectoris: platelet behavior and prognosis in progressive angina and intermediate coronary syndromes. Circulation 1990; 81 I I-16-9.
    PubMedGoogle Scholar
  • 22 Fitzgerald DJ, Roy L, Catella F, Fitzgerald GA. Platelet activation in unstable coronary artery disease. N Engl J Med 1986; 315: 983-9.
    CrossrefPubMedGoogle Scholar
  • 23 Ott I, Neumann F-J, Gawaz M, Schmitt M. Schö}mig A. Increased neutrophil-platelet adhesion in patients with unstable angina. Circulation 1996; 94: 1239-46.
    CrossrefPubMedGoogle Scholar
  • 24 Carry M, Korley V, Willerson JT, Weigelt L, Ford-Hutchinson AW, Tagari P. Increased urinary leukotriene excretion in patients with cardiac ischemia. Circulation 1002; 85: 230-6.
    PubMedGoogle Scholar
  • 25 Lefer AM, Weyrich AS, Buerke M. Role of selectins, a new family of adhesion molecules, in ischemia-reperfusion injury. Cardiovasc Res 1994; 28: 289-94.
    CrossrefPubMedGoogle Scholar
  • 26 Folts JD, Gallagher K, Rowe GG. Blood flow reductions in stenosed canine coronary arteries: vasospasm or platelet aggregation?. Circulation 1982; 65: 248-55.
    CrossrefPubMedGoogle Scholar
  • 27 Badimon L, Badimon JJ, Galvez A, Chesebro JH, Fuster V. Influence of arterial damage and wall shear rate on platelet deposition: ex vivo study in a swine model. Arteriosclerosis 1986; 6: 312-20.
    CrossrefPubMedGoogle Scholar
  • 28 Ogawa H, Yasue H, Oshima H, Okumura K, Matsuyama K, Obata K. Circadian variation of plasma fibrinopeptide A level in patients with variant angina. Circulation 1989; 80: 1617-26.
    CrossrefPubMedGoogle Scholar
  • 29 Harmon JJ, Jamieson GA. The glycocalicin portion affinity receptor sites for thrombin: a soluble radioreceptor assay for the interaction of thrombin with platelets. J Biol Chem 1986; 261: 13224-9.
    PubMedGoogle Scholar
  • 30 Mazilli M, Goldstein S, Trivella MG. et al. Some clinical considerations regarding the relation of coronary vasospasm to coronary atherosclerosis: A hypothetical pathogenesis. Am J Cardiol 1980; 45: 882-6.
    CrossrefPubMedGoogle Scholar
  • 31 Rentrop KP. Thrombolytic therapy in patients with acute myocardial infarction. Circulation 1985; 71: 627-31.
    CrossrefPubMedGoogle Scholar
  • 32 Fuster V, Chesebro JH. Mechanism of unstable angina. N Engl J Med 1986; 315: 1023-25.
    CrossrefPubMedGoogle Scholar
  • 33 Oliva PB, Broekinridge JC. Arteriographic evidence of coronary arterial spasm in acute myocardial infarction. Circulation 1977; 56: 366-74.
    CrossrefPubMedGoogle Scholar