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Hamostaseologie 2010; 30(04): 194-201
DOI: 10.1055/s-0037-1619055
Review
Schattauer GmbH

Immunoassays for diagnosis of coagulation disorders

Immunoassays für die Diagnose von Koagulationsstörungen
A. Kappel
1   Pre-Development, Siemens Healthcare Diagnostics Products GmbH, Marburg, Germany
,
M. Ehm
1   Pre-Development, Siemens Healthcare Diagnostics Products GmbH, Marburg, Germany
› Author Affiliations
Further Information

Publication History

Publication Date:
28 December 2017 (online)

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Summary

Immunoassays play a pivotal role in the clinical laboratory. In the coagulation section of the laboratory, they are used as an aid for diagnosis of deep vein thrombosis or pulmonary embolism, thrombophilia screening, or detection of coagulation factor deficiencies, respectively. Enzyme-linked immunosorbent assay (ELISA) and latex agglutination immunoassay technologies are currently most widely used, while Luminescent Oxygen Channeling Immuno - assay (LOCI®) and other chemiluminescencebased immunoassays are emerging technologies for the coagulation laboratory. However, not all immunoassay technologies employed are compatible with the workflow requirements of the coagulation laboratory, and, not all technologies are suitable for detection or quantification of every marker.

This review focuses on technical and performance aspects of those immunoassay technologies that are most widely used in the coagulation laboratory, and provides a description of markers that are typically tested by immunoassays.

Zusammenfassung

Immunoassays spielen im klinischen Labor eine herausragende Rolle. Im Gerinnungslabor werden sie unterstützend bei der Diagnose der tiefen Beinvenenthrombose und der Lungenembolie eingesetzt und beim Thrombophilie-Screening sowie der Ermittlung von Gerinnungsfaktorenmängeln verwendet. ELISA (enzyme-linked immunosorbent assay) und latexverstärkte Agglutinationstests werden gegenwärtig am häufigsten im Gerinnungslabor verwendet, während die Luminescent- Oxygen-Channeling-Immunoassay(LOCI®)Technologie und andere Chemilumineszenzbasierte Immunoassays aufkommende Technologien darstellen.

In diesem Übersichtsartikel werden technische und Leistungsaspekte der verschiedenen im Gerinnungslabor verwendeten Immunoassay-Technologien sowie die typischerweise mittels Immunoassays getesteten Marker vorgestellt.

Keywords

ELISA - immunoassay - luminescent oxygen channeling - latex agglutination

Schlüsselwörter

ELISA - Immunoassay - LOCI - Agglutinationstest
 

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