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Thromb Haemost 1989; 61(02): 178-182
DOI: 10.1055/s-0038-1646555
Original Article
Schattauer GmbH Stuttgart

Induction of Endothelial Cell/Macrophage Procoagulant Activity: Synergistic Stimulation by Gamma Interferon and Granulocyte-Macrophage Colony Stimulating Factor

Steven H Zuckerman
The Department of Immunology, Lilly Research Labs, Indianapolis, IN, USA
,
Yvonne M Surprenant
The Department of Immunology, Lilly Research Labs, Indianapolis, IN, USA
› Author Affiliations
Further Information

Publication History

Received 17 August 1988

Accepted after revision 10 November 1988

Publication Date:
30 June 2018 (online)

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Summary

Inflammatory mediators such as endotoxin can stimulate the expression of procoagulant activity on both endothelial cells and macrophages while the monokines Interleukin 1, IL-1, and Tumor Necrosis Factor, TNF induce procoagulant activity on endothelial cells. Incubation of murine peritoneal macrophages with suboptimal concentrations of endotoxin results in a two fold increase in procoagulant activity. Macrophages incubated with gamma interferon, IFN γ, or Granulocyte-Macrophage Colony Stimulating Factor, GM-CSF, for 16 hours prior to endotoxin stimulation demonstrated a synergistic increase in procoagulant activity. A synergistic increase in procoagulant activity was also observed with primary cultures of human umbilical cord endothelial cells incubated with recombinant human IFN γ for 16 hours prior to endotoxin, TNF, or IL-1 stimulation. Human GM-CSF had no stimulatory effect on endotoxin or monokine induced endothelial cell procoagulant activity. The augmentation of macrophage and endothelial cell procoagulant activity by IFN γ and GM-CSF may provide a novel explanation for the role of these cytokines in acute and chronic inflammation.

Keywords

IL-1 - TNF - Endotoxin - Inflammation - Tissue factor
 

  • References

  • 1 Nemerson Y, Bach R. Tissue factor revisited. Prog Haemostas Thromb 1982; 6: 237-261
    PubMedGoogle Scholar
  • 2 Guha A, Bach R, Konigsberg W, Nemerson Y. Affinity purification of human tissue factor: Interaction of factor VII and tissue factor in detergent micelles. Proc Natl Acad Sci USA 1986; 83: 299-302
    CrossrefPubMedGoogle Scholar
  • 3 Nemerson Y. The phospholipid requirement of tissue factor in blood coagulation. J Clin Invest 1968; 47: 72-80
    CrossrefPubMedGoogle Scholar
  • 4 Morrissey JH, Fakhrai H, Edgington TS. Molecular cloning of the cDNA for tissue factor, the cellular receptor for the initiation of the coagulation protease cascade. Cell 1987; 50: 129-135
    CrossrefPubMedGoogle Scholar
  • 5 Nemerson Y. An ordered addition, essential activation model of the tissue factor pathway of coagulation: Evidence for a conformational cage. Biochemistry 1986; 25: 4020-4033
    CrossrefPubMedGoogle Scholar
  • 6 Cole EH, Sweet J, Levy GA. Expression of macrophage procoagulant activity in murine systemic lupus erythematosus. J Clin Invest 1986; 78: 887-893
    CrossrefPubMedGoogle Scholar
  • 7 Cole EH, Schulman J, Urowitz M, Keystone E, Williams C, Levy G. Monocyte procoagulant activity in glomerulonephritis associated with systemic lupus erythematosus. J Clin Invest 1985; 75: 861-868
    CrossrefPubMedGoogle Scholar
  • 8 Rothberger H, Barringer M, Meredith J. Increased tissue factor activity of monocytes/macrophages isolated from canine renal allografts. Blood 1984; 63: 623-628
    PubMedGoogle Scholar
  • 9 Nawroth PP, Stem DM. Modulation of endothelial cell hemostatic properties by tumor necrosis factor. J Exp Med 1986; 163: 740-745
    CrossrefPubMedGoogle Scholar
  • 10 Brox JH, Osterud B, Bjorklid E, Fenton JW. Production and availability of thromboplastin in endothelial cells: The effects of thrombin, endotoxin and platelets. Br J Haematol 1984; 57: 239-246
    CrossrefPubMedGoogle Scholar
  • 11 Galdal KS. Thromboplastin synthesis in endothelial cells. Haemostasis 1984; 14: 378-385
    PubMedGoogle Scholar
  • 12 Colucci M, Balconi G, Lorenzet R, Pietra A, Locati D, Donati MB, Semeraro N. Cultured human endothelial cells generate tissue factor in response to endotoxin. J Clin Invest 1983; 71: 1893-1896
    CrossrefPubMedGoogle Scholar
  • 13 Johnsen UL H, Lyberg T, Galdal KS, Prydz H. Platelets stimulate thromboplastin synthesis in human endothelial cells. Thromb Haemostas 1983; 49: 69-72
    PubMedGoogle Scholar
  • 14 Bevilacqua MP, Pober JS, Majeau GR, Cotran RS, Gimbrone Jr MA. Interleukin 1 (IL-1) induced biosynthesis and cell surface expression of procoagulant activity in human vascular endothelial cells. J Exp Med 1984; 160: 618-623
    CrossrefPubMedGoogle Scholar
  • 15 Bevilacqua MP, Pober JS, Wheeler ME, Cotran RS, Gimbrone Jr MA. Interleukin-1 activation of vascular endothelium. Effects on procoagulant activity and leukocyte adhesion Am J Pathol 1985; 121: 393-403
    PubMedGoogle Scholar
  • 16 Nawroth PP, Handley DA, Esmon CT, Stern DM. Interleukin 1 induces endothelial cell procoagulant while suppressing cell-surface anticoagulant activity. Proc Natl Acad Sci USA 1986; 83: 3460-3464
    CrossrefPubMedGoogle Scholar
  • 17 Bevilacqua MP, Pober JS, Majeau GR, Fiers W, Cotran RS, Gimbrone Jr MA. Recombinant tumor necrosis factor induces procoagulant activity in cultured human vascular endothelium: Characterization and comparison with the actions of interleukin 1. Proc Natl Acad Sci USA 1986; 83: 4533-4537
    CrossrefPubMedGoogle Scholar
  • 18 Geczy CL, Hopper KE. A mechanism of migration inhibition in delayed-type hypersensitivity reactions. II. Lymphokines promote procoagulant activity of macrophages in vitro. J Immunol 1981; 126: 1059-1061
    PubMedGoogle Scholar
  • 19 Lyberg T, Stanley ER, Prydz H. Colony-Stimulating Factor-1 induces thromboplastin activity in murine macrophages and human monocytes. J Cell Physiol 1987; 132: 367-370
    CrossrefPubMedGoogle Scholar
  • 20 Helin H, Edgington TS. Allogeneic induction of the human T cell- instructed monocyte procoagulant response is rapid and is elicited by HLA-DR. J Exp Med 1983; 158: 962-975
    CrossrefPubMedGoogle Scholar
  • 21 Janco RL, Morris PJ. Regulation of monocyte procoagulant by chemoattractants. Blood 1985; 65: 545-552
    PubMedGoogle Scholar
  • 22 Gregory SA, Kornbluth RS, Helin H, Remold HG, Edgington TS. Monocyte procoagulant inducing factor: A lymphokine involved in the T cell-instructed monocyte procoagulant response to antigen. J Immunol 1986; 137: 3231-3239
    PubMedGoogle Scholar
  • 23 Ryan J, Geczy CL. Characterization and purification of mouse macrophage procoagulant-inducing factor. J Immunol 1986; 137: 2864-2870
    PubMedGoogle Scholar
  • 24 Muhlfelder TW, Niemetz J, Kang S. Glucocorticoids inhibit the generation of leukocyte procoagulant (tissue factor) activity. Blood 1982; 60: 1169-1172
    PubMedGoogle Scholar
  • 25 Galdal KS, Lyberg T, Evensen SA, Nilsen E, Prydz H. Inhibition of the thromboplastin response of endothelial cells in vitro. Biochem Pharmacol 1984; 33: 2723-2726
    CrossrefPubMedGoogle Scholar
  • 26 Zuckerman SH, Surprenant YM. Augmentation of procoagulant activity in monokine stimulated human endothelial cells by calmodu-lin/protein kinase C inhibitors. Thromb Res 1988; 49: 205-214
    CrossrefPubMedGoogle Scholar
  • 27 Levy GA, Edgington TS. Lymphocyte cooperation is required for amplification of macrophage procoagulant activity. J Exp Med 1980; 151: 1232-1244
    CrossrefPubMedGoogle Scholar
  • 28 Edwards RL, Rickies FR. The role of human T cells (and T cell products) for monocyte tissue factor generation. J Immunol 1980; 125: 606-609
    PubMedGoogle Scholar
  • 29 Jaffe EA, Nachman RL, Becker CG, Minick CR. Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria J Clin Invest 1973; 52: 2745-2756
    CrossrefPubMedGoogle Scholar
  • 30 Surprenant YM, Zuckerman SH. A novel microassay for the quantitation of procoagulant expression of adherent macrophage and endothelial cells. Thromb Res 1989; 53: 339-346
    CrossrefPubMedGoogle Scholar
  • 31 Zuckerman SH, Surprenant YM, Tang J. Synergistic effect of Granulocyte-Macrophage Colony-Stimulating Factor and 1,25-Dihydroxyvitamin D3on the differentiation of the human monocytic cell line U937. Blood 1988; 71: 619-624
    PubMedGoogle Scholar
  • 32 Adams DO, Hamilton TA. The cell biology of macrophage activation. Annu Rev Immunol 1984; 2: 283-318
    CrossrefPubMedGoogle Scholar
  • 33 Collart MA, Belin D, Vassalli JD, de Kossodo S, Vassalli P. Gamma interferon enhances macrophage transcription of the tumor necrosis factor/cachectin, interleukin 1, and urokinase genes, which are controlled by short-lived repressors. J Exp Med 1986; 164: 2113-2118
    CrossrefPubMedGoogle Scholar
  • 34 Collins T, Korman AJ, Wake CT, Boss JM, Kappes DJ, Fiers W, Ault KA, Gimbrone Jr MA, Strominger JL, Pober JS. Immune interferon activates multiple class II major histocompatibility complex genes and the associated invariant chain gene in human endothelial cells and dermal fibroblasts. Proc Natl Acad Sci USA 1984; 81: 4917-4921
    CrossrefPubMedGoogle Scholar
  • 35 Collins T, Lapierre LA, Fiers W, Strominger JL, Pober JS. Recombinant human tumor necrosis factor increases mRNA levels and surface expression of HLA-A,B antigens in vascular endothelial cells and dermal fibroblasts in vitro. Proc Natl Acad Sci USA 1986; 83: 446-450
    CrossrefPubMedGoogle Scholar
  • 36 Pober JS, Collins T, Gimbrone Jr MA, Libby P, Reiss CS. Inducible expression of class II major histocompatibility complex antigens and the immunogenicity of vascular endothelium. Transplant 1986; 41: 141-146
    CrossrefPubMedGoogle Scholar
  • 37 Miossec P, Ziff M. Immune interferon enhances the production of interleukin 1 by human endothelial cells stimulated with lipopolysaccharide. J Immunol 1986; 137: 2848-2852
    PubMedGoogle Scholar
  • 38 Kurt-Jones EA, Fiers W, Pober JS. Membrane interleukin 1 induction on human endothelial cells and dermal fibroblasts. J Immunol 1987; 139: 2317-2324
    PubMedGoogle Scholar
  • 39 Lapierre LA, Fiers W, Pober JS. Three distinct classes of regulatory cytokines control endothelial cell MHC antigen expression. Interactions with immune γ interferon differentiate the effects of tumor necrosis factor and lymphotoxin from those of leukocyte α and fibroblast β interferons J Exp Med 1988; 167: 794-804
    CrossrefPubMedGoogle Scholar
  • 40 Pober JS, Lapierre LA, Stolpen AH, Brock TA, Springer TA, Fiers W, Bevilacqua MP, Mendrick DL, Gimbrone Jr MA. Activation of cultured human endothelial cells by recombinant lymphotoxin: Comparison with tumor necrosis factor and interleukin 1 species. J Immunol 1987; 138: 3319-3324
    PubMedGoogle Scholar
  • 41 Montesano R, Orci L, Vassalli P. Human endothelial cell cultures: Phenotypic modulation by leukocyte interleukins. J Cell Physiol 1985; 122: 424-434
    CrossrefPubMedGoogle Scholar
  • 42 Metcalf D. The granulocyte-macrophage colony-stimulating factors. Science 1985; 229: 16-22
    CrossrefPubMedGoogle Scholar
  • 43 Handman E, Burgess AW. Stimulation by granulocyte-macrophage colony stimulating factor of Leishmania tropica killing by macrophages. Immunology 1979; 122: 1134-1137
    PubMedGoogle Scholar
  • 44 Weiser WY, Van Niel A, Clark SC, David JR, Remold HG. Human biosynthetic granulocyte macrophage colony stimulating factor (rt GM-CSF) activates human macrophages to kill Leishmania donovani. Fed Am Soc Exp Biol 1987; 45: 1387 a
    PubMedGoogle Scholar
  • 45 Hamilton JA, Stanley ER, Burgess AW, Shadduck RK. Stimulation of macrophage plasminogen activator activity by colony-stimulating factors. J Cell Physiol 1980; 103: 435-445
    CrossrefPubMedGoogle Scholar
  • 46 Grabstein KH, Urdal DL, Tushinski RJ, Mochizuki DY, Price VL, Cantrell MA, Gillis S, Conlon PJ. Induction of macrophage tumoricidal activity by granulocyte-macrophage colony stimulating factor. Science 1986; 232: 506-508
    CrossrefPubMedGoogle Scholar
  • 47 Zuckerman SH, Schreiber RD. Up-regulation of gamma interferon receptors on the human monocytic cell line U937 by 1,25 Dihydroxy- vitamin D3and Granulocyte-Macrophage colony stimulating factor. J Leuk Biol 1988; 44: 187-191
    CrossrefPubMedGoogle Scholar
  • 48 Bagby Jr GC, Dinarello CA, Wallace P, Wagner C, Hefeneider S, McCall E. Interleukin 1 stimulates Granulocyte Macrophage Colony- stimulating activity release by vascular endothelial cells. J Clin Invest 1986; 78: 1316-1323
    CrossrefPubMedGoogle Scholar
  • 49 Sieff CA, Schickwann T, Faller DV. Interleukin 1 induces cultured human endothelial cell production of Granulocye-Macrophage colony-stimulating factor. J Clin Invest 1987; 79: 48-51
    CrossrefPubMedGoogle Scholar
  • 50 Ruggiero V, Tavernier J, Fiers W, Baglioni C. Induction of the synthesis of tumor necrosis factor receptors by interferon-γ. J Immunol 1986; 136: 2445-2450
    PubMedGoogle Scholar
  • 51 Tsujimoto M, Yip YK, Vilcek J. Interferon-γ enhances expression of cellular receptors for tumor necrosis factor. J Immunol 1986; 136: 2441-2444
    PubMedGoogle Scholar