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Thromb Haemost 1989; 61(03): 493-496
DOI: 10.1055/s-0038-1646621
Original Article
Schattauer GmbH Stuttgart

The Effect of Lipopolysaccharides (LPS) on Generation of Thromboxane A2 and Thromboplastin Activity in Whole Blood of Males, Females and Females on Oral Contraceptives

John-Bjarne Hansen
*   The Institute of Clinical Medicine, University of Tromsø, Tromsø, Norway
,
Line Wilsgård
Institute of Medical Biology, University of Tromsø, Tromsø, Norway
,
Jan Ole Olsen
Institute of Medical Biology, University of Tromsø, Tromsø, Norway
,
Birgit Svensson
*   The Institute of Clinical Medicine, University of Tromsø, Tromsø, Norway
,
Bjarne Østerud
Institute of Medical Biology, University of Tromsø, Tromsø, Norway
› Author Affiliations
Further Information

Publication History

Received 07 June 1988

Accepted after revision 09 February 1989

Publication Date:
24 July 2018 (online)

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Summary

Heparinized blood sample taken from 81 healthy persons, 40 men and 41 women of whom 17 were on combined oral contraceptives, were incubated with 2 ng lipopolysaccharides (LPS)/ml blood. Quantitation of thromboxane B2 (TxB2) in the resultant plasma revealed a significantly (p <0.001) higher generation of TxB2 in men than in women. No sexual differences were observed in thromboplastin activity in unstimulated and LPS stimulated monocytes. Addition of liposomes together with LPS enhanced the synthesis of thromboplastin 2-10 fold and unveiled a higher synthesis of thromboplastin among men. No differences were observed in TxB2 and thromboplastin synthesis in blood collected from females or females using oral contraceptives. A significant correlation between TxB2 and the thromboplastin activity exists within the male (r = 0.46, p = 0.003) and the oral contraceptive (r = 0.57, p = 0.016) group after exposure to LPS. This study suggests that males exposed to LPS may generate more thromboxane A2 than females and thus be at higher risk of developing thrombosis.

The production of thromboxane A2 (TxA2) in platelets upon stimulation may influence the tendency to thrombosis by its proaggregating and vasoconstrictor effect. Although lipopolysaccharides (LPS) lack the ability to induce platelet aggregation in humans (10), Schwartz et al. have reported that mononuclear cells preincubated with LPS induce platelet aggregation most probably through thrombin generation at the surface of monocytes as a result of thromboplastin expression (11).

The purpose of this study was to investigate possible differences in the reactivity of whole blood to a weak stimuli of LPS between males, females and females using oral contraceptives.

Keywords

Sex differences - Thromboplastin synthesis - TxB2 - Serum lipids
 

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