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Thromb Haemost 1989; 62(02): 752-755
DOI: 10.1055/s-0038-1646896
Original Article
Schattauer GmbH Stuttgart

Stimulation of Plasmin Catalyzed Conversion of SingleChain to Two-Chain Urokinase-Type Plasminogen Activator by Sulfated Polysaccharides

Andrzej Rydzewski
The Department of Physiology, Hamamatsu University, School of Medicine, Hamamatsu-shi, Shizuoka-ken, Japan
,
Yumiko Takada
The Department of Physiology, Hamamatsu University, School of Medicine, Hamamatsu-shi, Shizuoka-ken, Japan
,
Akikazu Takada
The Department of Physiology, Hamamatsu University, School of Medicine, Hamamatsu-shi, Shizuoka-ken, Japan
› Author Affiliations
Further Information

Publication History

Received 20 February 1989

Accepted after revision 03 March 1989

Publication Date:
30 June 2018 (online)

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Summary

Influence of heparin, chondroitin sulfate C and dextran sulfate (MW 3,500 and 7,000) on plasmin catalyzed conversion of singlechain (scu-PA) to two-chain (u-PA) urokinase-type plasminogen activator and generation of plasmin in mixtures of scu-PA and Glu-plasminogen (Glu-plg) was investigated. Conversion of scu-PA to u-PA catalyzed by plasmin was enhanced by chondroitin sulfate C and heparin, maximally by 10-fold and 3-fold, respectively. Sulfated polysaccharides potentiated generation of plasmin in mixtures of Glu-plg and scu-PA, as well as enhanced plasminogenolytic activity of u-PA. The possible role of these interactions is discussed.

Keywords

Single-chain urokinase-type plasminogen activator - Two-chain urokinase-type plasminogen activator - Sulfated polysaccharides - Potentiation
 

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