The Effect of Fatty-acid Autoxidation Products on Blood Coagulation

Summary

Polyunsaturated fatty acids were allowed to autoxidise in air over 4 days. The water soluble oxidation products were extracted at daily intervals and tested for their effect on blood coagulation. After 1 day there was slight acceleration of the recalcification and RVV times, but from 2–4 days the extracts became increasingly inhibitory. The P.T. and P.T.T. were also inhibited. In the thrombin generation test the extracts delayed the appearance of thrombin, but the peak thrombin level was increased and its rate of decay was reduced. When added to phospholipid the extracts altered their coagulant activity. The presence of autoxidation products could account for some of the variable results obtained with different preparations of phospholipids.

• References

• 1 Biggs R, Macfarlane R. G. Human Blood Coagulation and Its Disorders. Blackwell Scientific Publications; Oxford: 1962: 74
• 2 Canoso R. T, Rodvien R, Scoon K, Levine P. H. Hydrogen peroxide and platelet function. Blood 1974; 43: 645
• 3 Dormandy T. L. Biological rancidification. Lancet II: 684 1969;
• 4 Dormandy T. L. The autoxidation of red cells (Annotation). British Journal of Haematology 1971; 20: 457
• 5 Fullerton H. W, Davie W. J, Anastasopoulos G. Relationship of alimentary lipaemia to blood coagulability. British Medical Journal 1953; II: 250
• 6 Gutteridge J. M. C. The consequences of lipid autoxidation in biological material. Ph. D Thesis.; London University.: 1974
• 7 Gutteridge J. M. C, Lamport P, Dormandy T. L. Autoxidation as a cause of antibacterial activity in unsaturated fatty acids. Journal of Medical Microbiology 1974; 7: 387
• 8 Kune P, Klesse P. Zur chemischen Constitution der Lipoide der Gewebethrombokinase. Hoppe-Seylers Zeitschrift für physiologische Chemie 1958; 312: 214
• 9 Mickel H. S, Horbar J. The effect of peroxidised arachidonic acid upon human platelet aggregation. Lipids 1974; 9: 68
• 10 O’Brien Relation of blood coagulation to lipaemia. Lancet 1955; II: 690
• 11 O’Brien The effect of some fatty acids and phospholipids on blood coagulation. British Journal of Experimental Pathology 1957; 38: 529
• 12 Okumo M, Steiner M, Baldini M. G. Studies on lipid peroxides in platelets. II. Effect of aggregating agents and platelet antibody.. Journal of Laboratory and Clinical Medicine 1971; 77: 729
• 13 Poole J.C.F. The effect of certain fatty acids on the coagulation of plasma in vitro. British Journal of Experimental Pathology 1955; 36: 248
• 14 Rouser G, White S. G, Schloredt D. Phospholipid structure and thromboplastin activity. I. The phosphatide fraction active in recalcified normal human plasma.. Biochimica et Biophysica Acta 1958; 28: 71
• 15 Schauenstein E. Autoxidation of polyunsaturated esters in water: chemical structure and biological activity of products. Journal of Lipid Research 1967; 8: 417
• 16 Slater T. F. Free Radical mechanisms in tissue injury. Pion; London: 1972
• 17 Stocks J, Offerman E. L, Modell C. B, Dormandy T. L. The susceptibility to autoxidation of human red cell lipids in health and disease. British Journal of Haematology 1972; 23: 713
• 18 Stocks J, Guttteridge J. M. C, Sharp R. J, Dormandy T. L. The inhibition of lipid autoxidation by human serum and its relation to serum proteins and α-tocopherol. Clinical Science and Molecular Medicine 1974; 47: 223