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Planta Med 2017; 83(06): 534-544
DOI: 10.1055/s-0042-119652
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Antidiabetic and Antihyperalgesic Effects of a Decoction and Compounds from Acourtia thurberi

Ana Laura Martínez
1   Departamento de Farmacia, Facultad de Química, Universidad Nacional Autónoma de México, Ciudad de México, México
,
Abraham Madariaga-Mazón
1   Departamento de Farmacia, Facultad de Química, Universidad Nacional Autónoma de México, Ciudad de México, México
,
Isabel Rivero-Cruz
1   Departamento de Farmacia, Facultad de Química, Universidad Nacional Autónoma de México, Ciudad de México, México
,
Robert Bye
2   Jardín Botánico, Instituto de Biología, Universidad Nacional Autónoma de México, Ciudad de México, México
,
Rachel Mata
1   Departamento de Farmacia, Facultad de Química, Universidad Nacional Autónoma de México, Ciudad de México, México
› Author Affiliations
Further Information

Publication History

received 12 August 2016
revised 11 October 2016

accepted 17 October 2016

Publication Date:
04 November 2016 (online)

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Abstract

The purpose of this research was to examine the preclinical efficacy of a decoction from the roots of Acourtia thurberi as a hypoglycemic, antihyperglycemic, and antihyperalgesic agent using well-known experimental models in mice. Acute oral administration of A. thurberi decoction did not produce toxic effects in mice, according to the Lorke procedure. A. thurberi decoction (31.6–316.2 mg/kg, p. o.) decreased blood glucose levels during acute hypoglycemic and the oral glucose tolerance and oral sucrose tolerance tests, both in normoglycemic and hyperglycemic animals. Phytochemical analysis of A. thurberi roots led to the isolation of perezone (1), a mixture of α-pipitzol (2) and β-pipitzol (3), and 8-β-D-glucopyranosyloxy-4-methoxy-5-methyl-coumarin (4). A pharmacological evaluation of compounds 1–4 (3.2–31.6 mg/kg) using the same assays revealed their hypoglycemic and antihyperglycemic actions. Finally, local administration of A. thurberi decoction (31.6–316.2 µg/paw) and compounds 1–4 (3.2–31.6 µg/paw) produced significant inhibition on the licking time during the formalin test in healthy and hyperglycemic mice, demonstrating their antinociceptive and antihyperalgesic potential, respectively. Altogether, these results could be related to the use of A. thurberi for treating diabetes and painful complaints in contemporary Mexican folk medicine. A suitable UPLC-ESI/MS method was developed and successfully applied to quantify simultaneously compounds 1 and 4 in A. thurberi decoction.

Key words

Acourtia thurberi - Asteraceae - hypoglycemic activity - antihyperalgesic action - perezone - α-pipitzol - β-pipitzol

Supporting Information

Spectroscopic data of compounds 1–4 are available as Supporting Information.

 

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