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Planta Med 2018; 84(05): 311-319
DOI: 10.1055/s-0043-121270
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Cyclopia Extracts Enhance Th1-, Th2-, and Th17-type T Cell Responses and Induce Foxp3+ Cells in Murine Cell Culture

Shiho Murakami
1   Department of Applied Biological Science, Tokyo University of Agriculture and Technology, Tokyo, Japan
,
Yutaka Miura
1   Department of Applied Biological Science, Tokyo University of Agriculture and Technology, Tokyo, Japan
,
Makoto Hattori
1   Department of Applied Biological Science, Tokyo University of Agriculture and Technology, Tokyo, Japan
,
Hiroshi Matsuda
2   Division of Animal Life Science, Tokyo University of Agriculture and Technology, Tokyo, Japan
,
Christiaan J. Malherbe
3   Plant Bioactives Group, Post-Harvest and Agro-Processing Technologies Division, Agricultural Research Council, Stellenbosch, South Africa
,
Christo J. F. Muller
4   Biomedical Research and Innovation Platform, South African Medical Research Council, Tygerberg, South Africa
,
Elizabeth Joubert
3   Plant Bioactives Group, Post-Harvest and Agro-Processing Technologies Division, Agricultural Research Council, Stellenbosch, South Africa
5   Department of Food Science, Stellenbosch University, Stellenbosch, South Africa
,
Tadashi Yoshida
1   Department of Applied Biological Science, Tokyo University of Agriculture and Technology, Tokyo, Japan
› Author Affiliations
Further Information

Publication History

received 02 June 2017
revised 03 October 2017

accepted 06 October 2017

Publication Date:
02 November 2017 (online)

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Abstract

Cyclopia genistoides, one of the traditional South African medicinal plants, and other species of the same genus offer noteworthy phenolic profiles, in particular high levels of the anti-allergic xanthone mangiferin. Hot water and 40% ethanol-water (v/v) extracts, prepared from C. genistoides, Cyclopia subternata, and Cyclopia maculata, were tested for immune-regulating activity in vitro using murine splenocytes and mesenteric lymph node cells. The 40% ethanol-water extracts of C. genistoides and C. subternata significantly enhanced production of several types of cytokines, including IL-4, IL-17, and IFN-γ, by antigen-stimulated splenocytes. A concentration-dependent response was observed, noticeably for IFN-γ production. The activity of the extracts did not correlate with the content of any of the major phenolic compounds, indicative that other extract constituents also play a role in immunomodulation. Additionally, the increased ratio of CD4+CD25+Foxp3+ Treg cells to total CD4+ cells indicated induction of Foxp3+ cells when mesenteric lymph node cells were cultured in the presence of these two extracts. This study is the first reporting immunostimulatory activity for Cyclopia, which are widely consumed as the herbal tea known as honeybush, underpinning further investigations into the potential use of its extracts as adjuvants for mucosal immunotherapy.

Key words

Cyclopia Vent. - cytokines - Foxp3+ - T cell proliferation - Th1 - Th2 - Th17
 

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