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Aktuelle Urol 2002; 33(3): 206-212
DOI: 10.1055/s-2002-32114
Experimentelle Originalarbeit
© Georg Thieme Verlag Stuttgart · New York

Untersuchungen zur unterschiedlichen Karzinominzidenz von Prostata und Samenblasen

Evaluation of the Different Cancer Incidence in Prostate and Seminal VesiclesJ.  Pannek1 , A.  W.  Partin2
  • 1Urologische Klinik der Ruhr-Universität Bochum, Marienhospital Herne
  • 2James Buchanan Brady Urological Institute, The Johns Hopkins Medical Institution, Baltimore, Maryland, USA
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Publication History

Publication Date:
07 June 2002 (online)

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Zusammenfassung

Fragestellung: Samenblasen und Prostata gehören zu den akzessorischen Geschlechtsdrüsen, werden durch ihre räumliche Nähe mit denselben potenziellen Karzinogenen konfrontiert und unterliegen beide einer androgenen Steuerung. Dennoch ist die Karzinominzidenz dieser Organe dramatisch unterschiedlich. In der vorliegenden Studie sollte der Einfluss verschiedener Faktoren auf die Karzinogenese dieser Organe evaluiert werden. Material und Methode: An je 20 Präparaten von Samenblasen und Prostatagewebe wurden immunhistochemisch die Expression von Metallothionein, Glutathion S-Transferase, Chromogranin-A, TGF-β1, bcl-2 und MIB-1 sowie die Apoptoserate semiquantitativ bestimmt. Bei jeweils 10 Patienten wurden Zellkernmorphometrie und zweidimensionale Kernmatrixprotein-Gelelektrophoresen durchgeführt. Ergebnisse: Prostata und Samenblasen stellen das bisher einzige Organpaar dar, die keine organspezifische Kernmatrixproteinstruktur aufweisen. Die Kernmorphometrie der Samenblasen ist variabler als die der Prostata. Der Zellumsatz ist in der Prostata vielfach höher als in Samenblasen. Die Steuerung der Apoptose erfolgt in Samenblasen hauptsächlich durch TGF-β1. Neuroendokrine Zellen waren in den Samenblasen nicht nachweisbar. Die Expression von Glutathion-S-Transferase war in beiden Gruppen gleich, die von Metallothionein in der Prostata stärker als in den Samenblasen. Schlussfolgerung: Die unterschiedliche Karzinominzidenz in den beiden Organen beruht weniger auf unterschiedlicher Expression tumorprotektiver Enzyme. Der geringere Zellumsatz der Samenblasen in Verbindung mit der Zellkernmorphologie wiesen auf einen unterschiedlichen Differenzierungsgrad dieser Organe hin.

Abstract

Purpose: The human prostate and seminal vesicles are both androgen-dependent accessory sex organs. They share the same blood supply, exposure to carcinogens and androgen stimulation. Despite these similarities, the incidence of cancer in these two organs is vastly different. We analysed the impact of various factors on the carcinogenesis of these tissues. Materials and Methods: In 20 specimens each of seminal vesicles and prostatic tissue, expression of metallothioneine, glutathione S-transferase (GST), chromogranin-A, TGF-β1, bcl-2 and MIB-1, as well as apoptotic rate were assessed by immunohistochemistry. In 10 specimens each, nuclear morphometry and two-dimensional gel electrophoresis of nuclear matrix proteins (NMP) were performed. Results: NMP composition analysis demonstrated that both tissues had a similar NMP composition. Tissue-specific NMPs consistently present in all specimens of each tissue could not be demonstrated. Nuclear morphometry showed a significantly greater heterogeneity in nuclear shape in the seminal vesicles than in the prostate. Cell turnover was higher in the prostate than in the seminal vesicles. TGF-β1 seems to be more important for the regulation of cell turnover in the seminal vesicles than bcl-2. Neuroendocrine cells were not detected in the seminal vesicles, while GST-expression was similar in both tissues. Metallothioneine expression is lower in the seminal vesicles than in the prostate. Conclusions: Expression of tumor-protective proteins cannot be regarded as the main reason for the vastly different cancer incidence in the seminal vesicles and prostate. The low cell turnover and heterogeneous nuclear morphology of the seminal vesicles may suggest that the seminal vesicle tissue is well-differentiated.

Schlüsselwörter

Prostata - Karzinogenese - Samenblasen - Zellumsatz - Prostatakarzinom

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Privatdozent Dr. med. J. Pannek

Oberarzt der Urologischen Klinik der Ruhr-Universität Bochum · Marienhospital Herne

Widumer Str. 8

44627 Herne

Phone: 02323/499-0

Fax: 02323/499-388

Email: juergen.pannek@cityweb.de

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