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Horm Metab Res 2005; 37(1): 40-44
DOI: 10.1055/s-2005-861035
Original Clinical
© Georg Thieme Verlag KG Stuttgart · New York

Acute Insulin Responses to Intravenous Glucose and GLP-1 are Independent of Preceding High-frequency Insulin Pulse-defects Induced by Glucose Entrainment in Healthy Humans

C.  B.  Juhl1, 2 , J.  Sturis3 , M.  Hollingdal1 , O.  Schmitz1, 2
  • 1Medical Department M (Endocrinology and Diabetes), University Hospital of Århus, Denmark
  • 2Institute of Clinical Pharmacology, University of Århus, Denmark
  • 3Pharmacology, Novo Nordisk A/S, Bagsværd, Denmark
Further Information

Publication History

Received 23 March 2004

Accepted after Revision 15 September 2004

Publication Date:
09 February 2005 (online)

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Abstract

The objective of this study was to test the hypothesis that high-frequency oscillations in insulin release is a part of the mechanistic basis of a prompt and adequate insulin response to iv-glucose and GLP-1 exposure. In ten healthy subjects, five different insulin release patterns were induced for 360 min using computer-based glucose infusion (glucose delivered in a constant, a regular pulsatile, an irregular pulse frequency, an irregular pulse amplitude or a regular but very fast-pulsatile manner) in healthy subjects. The amount of glucose infused was identical in all five protocols (24 mg/kg/h). After 360 min, insulin secretion was assessed by means of a first-phase insulin secretion test (25 g glucose) and injection of GLP-1 (9 μg). By frequent blood sampling and analysis of insulin concentration, glucose-induced entrainment was evident in all protocols except in the constant infusion and the very fast-pulse protocol. The first-phase insulin release to glucose and GLP-1-induced insulin release were, however, comparable in the protocols. We therefore conclude from this short-term experimental setting in healthy subjects that beta-cell response to either iv-glucose or GLP-1 is independent of the preceding regularity of oscillations in insulin release.

Key words

Insulin secretion - Entrainment - Pulsatility - Human

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C. B. Juhl

Medical Department M (Endocrinology and Diabetes), University Hospital of Århus ·

Noerrebrogade 44 · 8000 Århus C · Denmark

Email: claus.juhl@dadlnet.dk

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