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Planta Med 1987; 53(2): 161-164
DOI: 10.1055/s-2006-962662
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© Georg Thieme Verlag Stuttgart · New York

Isolation of Vitamin D3 Metabolites from Solanum malacoxylon Leaf Extracts Incubated with Ruminal Fluid

R. L. Boland1 , M. I. Skliar1 , A. W. Norman2
  • 1Departamento de Biologia, Universidad Nacional del Sur, 8000 Bahia Blanca, Argentina.
  • 2Department of Biochemistry, University of California, Riverside, California 92521, U.S.A.
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Publication History

1986

Publication Date:
24 January 2007 (online)

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Abstract

Two fractions which elute similarly as 1,25(OH)2D3 and 1,24,25(OH)3D3 were isolated by TLC, Sephadex LH-20 chromatog-raphy and HPLC from Solanum malacoxylon leaf extracts preincubated in vitro with ruminal fluid. Both fractions effectively competed with [3 H]-1,25(OH)2D3 for binding to the chick intestinal 1,25(OH)2D3 receptor. Only significant amounts of the fraction which coelutes with 1,25(OH)2D3 were detected in leaf extracts incubated with purified β-glucosidase. Incubation of [3 H]-1,25(OH)2D3 with ruminai fluid resulted in the formation of a metabolite with similar mobility as 1,24,25(OH)3D3 in TLC plates. The results suggest that 1,25(OH)2D3-glycoside present in S. malacoxylon is converted to 1,25 (OH)2D3 and a more polar metabolite, possibly 1,24,25(OH)3D3, by the action of rumen microbes.

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