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Planta Med 1981; 43(10): 179-182
DOI: 10.1055/s-2007-971496
Research Articles

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HPCL Separation and Quantitative Determination of Valepotriates from Valeriana kilimandascharica

S. F. Dossaji1 , 2 , H. Becker2
  • 1Institut für Pharmazeutische Biologie der Universität Heidelberg, Heidelberg, Federal Republic of Germany
  • 2Dept. of Botany, University of Nairobi, Kenya.
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Publication History

Publication Date:
30 March 2007 (online)

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Abstract

Valepotriates, mainly isovaltrate and valtrate, have been separated and quantitatively estimated by reversed-phase HPLC in the leaves, flowers, stems and rhizomes of Valeriana kilimandascharica. The isovaltrate/valtrate concentration reaches a maximum of 5.89% in the leaves, 3.84% in the flowers, 3.17% in the stems and 5.15% in the rhizomes.

A µ Bondapak C18 column using MeOH-H2O mixtures as eluant is suitable for a baseline separation of isovaltrate, valtrate, acevaltrate and baldrinal at UV 254 nm in 15 min and didrovaltrate and IVHD-valtrat at UV 208 nm in 10 min. Relative standard deviation for quantitative determinations is approximately 1.5% for valepotriate contents of 1%. This method is adaptable for routine analysis of crude extracts.

Key Word Index

Valeriana - Valerianaceae - Valepotriates - HPLC

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